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Article

Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice

  • Authors:
    • Wei-Dong Xie
    • Hua Wang
    • Jin-Fang Zhang
    • Jian-Na Li
    • Yi Can
    • Lv Qing
    • Hsiang-Fu Kung
    • Ya-Ou Zhang
  • View Affiliations / Copyright

    Affiliations: Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, P.R. China, Stanley Ho Centre for Emerging Infections, The Chinese University of Hong Kong, Hong Kong, SAR, P.R. China
  • Pages: 309-315
    |
    Published online on: January 20, 2011
       https://doi.org/10.3892/etm.2011.208
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Abstract

This study aimed to investigate the potential mechanisms of natural resistance to high-fat diet-induced obesity. Four-week-old C57BL/6 mice were fed a high-fat diet for 6 weeks and were then designated as high-fat diet-fed obesity-prone (HOP) and obesity-resistant (HOR) animals. Their blood biochemistry was evaluated, and visceral adipose tissue samples were subjected to proteomic, Western blot and quantitative real-time PCR (q-PCR) analyses. The HOR mice showed reduced visceral fat weight and size, as well as lowered serum lipid and leptin levels. Proteomic analysis showed that enoyl coenzyme A hydratase 1, peroxisomal (Ech1) expression was significantly increased in their visceral adipose tissues. Moreover, other proteins, such as α-tropomyosin, myosin light chain, urine-nucleoside phosphorylase and transgelin, were also significantly increased. Furthermore, q-PCR analysis showed that the expression of acyl-CoA oxidase 1 palmitoyl, enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase and 3-oxoacyl-CoA thiolase responsible for peroxisomal β-oxidation was also up-regulated in the visceral adipose tissues of the HOR mice. The expression of peroxisome proliferator-activated receptor α (PPARα) was increased in the HOR mice as shown by Western blot analysis. Obesity-resistant animals show enhanced peroxisomal β-oxidation metabolism and reduced fat accumulation in visceral adipose tissues by up-regulating the expression of Ech1, peroxisomal or other related peroxisomal β-oxidation marker genes, which may be driven or enhanced by the up-regulation of the expression of PPARα. However, further validation in future studies is required.
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1. 

Nguyen DM and El-Serag HB: The epidemiology of obesity. Gastroenterol Clin North Am. 39:1–7. 2010. View Article : Google Scholar

2. 

Jia WP, Wang C, Jiang S and Pan JM: Characteristics of obesity and its related disorders in China. Biomed Environ Sci. 23:4–11. 2010. View Article : Google Scholar : PubMed/NCBI

3. 

Schrauwen P and Westerterp KR: The role of high-fat diets and physical activity in the regulation of body weight. Br J Nutr. 84:417–427. 2000. View Article : Google Scholar : PubMed/NCBI

4. 

Pagliassotti MJ, Gayles EC and Hill JO: Fat and energy balance. Ann NY Acad Sci. 827:431–448. 1997. View Article : Google Scholar : PubMed/NCBI

5. 

Nair RP and Ren J: Pharmacotherapy of obesity – benefit, bias and hyperbole. Curr Med Chem. 16:1888–1897. 2009.

6. 

Jackman MR, MacLean PS and Bessesen DH: Energy expenditure in obesity-prone and obesity-resistant rats before and after the introduction of a high-fat diet. Am J Physiol Regul Integr Comp Physiol. 299:R1097–R1105. 2010. View Article : Google Scholar : PubMed/NCBI

7. 

Takahashi N, Patel HR, Qi Y, et al: Divergent effects of leptin in mice susceptible or resistant to obesity. Horm Metab Res. 34:691–697. 2002. View Article : Google Scholar : PubMed/NCBI

8. 

Primeaux SD, Barnes MJ and Bray GA: Olfactory bulbectomy increases food intake and hypothalamic neuropeptide Y in obesity-prone but not obesity-resistant rats. Behav Brain Res. 180:190–196. 2007. View Article : Google Scholar : PubMed/NCBI

9. 

Ji H and Friedman MI: Reduced hepatocyte fatty acid oxidation in outbred rats prescreened for susceptibility to diet-induced obesity. Int J Obesity. 32:1331–1334. 2008. View Article : Google Scholar : PubMed/NCBI

10. 

Trinder P: Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann Clin Biochem. 6:24–27. 1969. View Article : Google Scholar : PubMed/NCBI

11. 

Allain CC, Poon LC, Chan CS, et al: Enzymatic determination of total cholesterol. Clin Chem. 20:470–475. 1974.PubMed/NCBI

12. 

Fossati P and Prencipe L: Serum triglycerides determined colorimetrically with an enzyme that produce hydrogen peroxide. Clin Chem. 28:2077–2080. 1982.PubMed/NCBI

13. 

Izzo C, Grillo F and Murador E: Improved method for determination of high-density-lipoprotein cholesterol I. Isolation of high-density lipoproteins by use of polyethylene glycol 6000. Clin Chem. 27:371–374. 1981.PubMed/NCBI

14. 

Kerscher L, Schiefer S, Draeger B, et al: Precipitation methods for the determination of LDL-cholesterol. Clin Biochem. 18:118–125. 1985. View Article : Google Scholar : PubMed/NCBI

15. 

Xie WD, Wang H, Zhang JF, et al: Proteomic profile of visceral adipose tissues between low-fat diet-fed obesity-resistant and obesity-prone C57BL/6 mice. Mol Med Rep. 3:1047–1052. 2010.PubMed/NCBI

16. 

Guo Y, Jolly RA, Halstead BW, et al: Underlying mechanisms of pharmacology and toxicity of a novel PPAR agonist revealed using rodent and canine hepatocytes. Toxicol Sci. 96:294–309. 2007. View Article : Google Scholar : PubMed/NCBI

17. 

Pagliassotti MJ, Knobel SM, Shahrokhi KA, et al: Time course of adaptation to a high-fat diet in obesity-resistant and obesity-prone rats. Am J Physiol. 267:R659–R664. 1994.PubMed/NCBI

18. 

Xie WD, Zhang YO, Wang NL, et al: Novel effects of macrostemonoside A, a compound from Allium macrostemon Bung, on hyperglycemia, hyperlipidemia, and visceral obesity in high-fat diet-fed C57BL/6 mice. Eur J Pharmacol. 599:159–165. 2008.PubMed/NCBI

19. 

Trayhurn P and Beattie JH: Physiological role of adipose tissue: white adipose tissue as an endocrine and secretory organ. Proc Nutr Soc. 60:329–339. 2001. View Article : Google Scholar : PubMed/NCBI

20. 

Rosicka M, Krsek M, Matoulek M, et al: Serum ghrelin levels in obese patients: the relationship to serum leptin levels and soluble leptin receptor levels. Physiol Res. 52:61–66. 2003.PubMed/NCBI

21. 

Luo JD, Zhang GS and Chen MS: Leptin and cardiovascular diseases. Timely Top Med Cardiovasc Dis. 9:E342005.PubMed/NCBI

22. 

Kannel WB and Vasan RS: Triglycerides as vascular risk factors: new epidemiologic insights. Curr Opin Cardiol. 24:345–350. 2009. View Article : Google Scholar : PubMed/NCBI

23. 

Swinburn B, Sacks G and Ravussin E: Increased food energy supply is more than sufficient to explain the US epidemic of obesity. Am J Clin Nutr. 90:1453–1456. 2009. View Article : Google Scholar : PubMed/NCBI

24. 

Kaplan LM: Pharmacologic therapies for obesity. Gastroenterol Clin North Am. 39:69–79. 2010. View Article : Google Scholar

25. 

Huzarewich RL, Siemens CG and Booth SA: Application of “omics” to prion biomarker discovery. J Biomed Biotechnol. 2010:6135042010.

26. 

Halvorsen YD, Wilkison WO and Briggs MR: Human adipocyte proteomics – a complementary way of looking at fat. Pharmacogenomics. 1:179–185. 2000.

27. 

FitzPatrick DR, Germain-Lee E and Valle D: Isolation and characterization of rat and human cDNAs encoding a novel putative peroxisomal enoyl-CoA hydratase. Genomics. 27:457–466. 1995. View Article : Google Scholar : PubMed/NCBI

28. 

Aoyama T, Peters JM, Iritani N, et al: Altered constitutive expression of fatty acid-metabolizing enzymes in mice lacking the peroxisome proliferator-activated receptor alpha (PPARalpha). J Biol Chem. 273:5678–5684. 1998. View Article : Google Scholar

29. 

Xie WD, Nie Y, Du LJ, et al: Preventive effects of fenofibrate on insulin resistance, hyperglycaemia, visceral fat accumulation in NIH mice induced by small-dose streptozotocin and lard. Pharmacol Res. 55:392–399. 2007. View Article : Google Scholar : PubMed/NCBI

30. 

Abbott BD: Review of the expression of peroxisome proliferator-activated receptors alpha (PPAR alpha), beta (PPAR beta), and gamma (PPAR gamma) in rodent and human development. Reprod Toxicol. 27:246–257. 2009. View Article : Google Scholar : PubMed/NCBI

31. 

Miyazaki M, Nakagawa I, Koga S, et al: Proteomics analysis of cardiac muscle from rats with peroxisomal proliferator-activated receptor alpha (PPARalpha) stimulation. J Toxicol Sci. 35:131–135. 2010. View Article : Google Scholar

32. 

Tsai YS and Maeda N: PPARgamma: a critical determinant of body fat distribution in humans and mice. Trends Cardiovasc Med. 15:81–85. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xie W, Wang H, Zhang J, Li J, Can Y, Qing L, Kung H and Zhang Y: Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice. Exp Ther Med 2: 309-315, 2011.
APA
Xie, W., Wang, H., Zhang, J., Li, J., Can, Y., Qing, L. ... Zhang, Y. (2011). Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice. Experimental and Therapeutic Medicine, 2, 309-315. https://doi.org/10.3892/etm.2011.208
MLA
Xie, W., Wang, H., Zhang, J., Li, J., Can, Y., Qing, L., Kung, H., Zhang, Y."Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice". Experimental and Therapeutic Medicine 2.2 (2011): 309-315.
Chicago
Xie, W., Wang, H., Zhang, J., Li, J., Can, Y., Qing, L., Kung, H., Zhang, Y."Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice". Experimental and Therapeutic Medicine 2, no. 2 (2011): 309-315. https://doi.org/10.3892/etm.2011.208
Copy and paste a formatted citation
x
Spandidos Publications style
Xie W, Wang H, Zhang J, Li J, Can Y, Qing L, Kung H and Zhang Y: Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice. Exp Ther Med 2: 309-315, 2011.
APA
Xie, W., Wang, H., Zhang, J., Li, J., Can, Y., Qing, L. ... Zhang, Y. (2011). Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice. Experimental and Therapeutic Medicine, 2, 309-315. https://doi.org/10.3892/etm.2011.208
MLA
Xie, W., Wang, H., Zhang, J., Li, J., Can, Y., Qing, L., Kung, H., Zhang, Y."Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice". Experimental and Therapeutic Medicine 2.2 (2011): 309-315.
Chicago
Xie, W., Wang, H., Zhang, J., Li, J., Can, Y., Qing, L., Kung, H., Zhang, Y."Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice". Experimental and Therapeutic Medicine 2, no. 2 (2011): 309-315. https://doi.org/10.3892/etm.2011.208
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