Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice

Xie,Wei-Dong; Wang,Hua; Zhang,Jin-Fang; Li,Jian-Na; Can,Yi; Qing,Lv; Kung,Hsiang-Fu; Zhang,Ya-Ou

doi:10.3892/etm.2011.208

Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice

Authors:
- Wei-Dong Xie
- Hua Wang
- Jin-Fang Zhang
- Jian-Na Li
- Yi Can
- Lv Qing
- Hsiang-Fu Kung
- Ya-Ou Zhang
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Affiliations: Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, P.R. China, Stanley Ho Centre for Emerging Infections, The Chinese University of Hong Kong, Hong Kong, SAR, P.R. China
Published online on: January 20, 2011 https://doi.org/10.3892/etm.2011.208
Pages: 309-315

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Abstract

This study aimed to investigate the potential mechanisms of natural resistance to high-fat diet-induced obesity. Four-week-old C57BL/6 mice were fed a high-fat diet for 6 weeks and were then designated as high-fat diet-fed obesity-prone (HOP) and obesity-resistant (HOR) animals. Their blood biochemistry was evaluated, and visceral adipose tissue samples were subjected to proteomic, Western blot and quantitative real-time PCR (q-PCR) analyses. The HOR mice showed reduced visceral fat weight and size, as well as lowered serum lipid and leptin levels. Proteomic analysis showed that enoyl coenzyme A hydratase 1, peroxisomal (Ech1) expression was significantly increased in their visceral adipose tissues. Moreover, other proteins, such as α-tropomyosin, myosin light chain, urine-nucleoside phosphorylase and transgelin, were also significantly increased. Furthermore, q-PCR analysis showed that the expression of acyl-CoA oxidase 1 palmitoyl, enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase and 3-oxoacyl-CoA thiolase responsible for peroxisomal β-oxidation was also up-regulated in the visceral adipose tissues of the HOR mice. The expression of peroxisome proliferator-activated receptor α (PPARα) was increased in the HOR mice as shown by Western blot analysis. Obesity-resistant animals show enhanced peroxisomal β-oxidation metabolism and reduced fat accumulation in visceral adipose tissues by up-regulating the expression of Ech1, peroxisomal or other related peroxisomal β-oxidation marker genes, which may be driven or enhanced by the up-regulation of the expression of PPARα. However, further validation in future studies is required.

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1.	Nguyen DM and El-Serag HB: The epidemiology of obesity. Gastroenterol Clin North Am. 39:1–7. 2010. View Article : Google Scholar
2.	Jia WP, Wang C, Jiang S and Pan JM: Characteristics of obesity and its related disorders in China. Biomed Environ Sci. 23:4–11. 2010. View Article : Google Scholar : PubMed/NCBI
3.	Schrauwen P and Westerterp KR: The role of high-fat diets and physical activity in the regulation of body weight. Br J Nutr. 84:417–427. 2000. View Article : Google Scholar : PubMed/NCBI
4.	Pagliassotti MJ, Gayles EC and Hill JO: Fat and energy balance. Ann NY Acad Sci. 827:431–448. 1997. View Article : Google Scholar : PubMed/NCBI
5.	Nair RP and Ren J: Pharmacotherapy of obesity – benefit, bias and hyperbole. Curr Med Chem. 16:1888–1897. 2009.
6.	Jackman MR, MacLean PS and Bessesen DH: Energy expenditure in obesity-prone and obesity-resistant rats before and after the introduction of a high-fat diet. Am J Physiol Regul Integr Comp Physiol. 299:R1097–R1105. 2010. View Article : Google Scholar : PubMed/NCBI
7.	Takahashi N, Patel HR, Qi Y, et al: Divergent effects of leptin in mice susceptible or resistant to obesity. Horm Metab Res. 34:691–697. 2002. View Article : Google Scholar : PubMed/NCBI
8.	Primeaux SD, Barnes MJ and Bray GA: Olfactory bulbectomy increases food intake and hypothalamic neuropeptide Y in obesity-prone but not obesity-resistant rats. Behav Brain Res. 180:190–196. 2007. View Article : Google Scholar : PubMed/NCBI
9.	Ji H and Friedman MI: Reduced hepatocyte fatty acid oxidation in outbred rats prescreened for susceptibility to diet-induced obesity. Int J Obesity. 32:1331–1334. 2008. View Article : Google Scholar : PubMed/NCBI
10.	Trinder P: Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann Clin Biochem. 6:24–27. 1969. View Article : Google Scholar : PubMed/NCBI
11.	Allain CC, Poon LC, Chan CS, et al: Enzymatic determination of total cholesterol. Clin Chem. 20:470–475. 1974.PubMed/NCBI
12.	Fossati P and Prencipe L: Serum triglycerides determined colorimetrically with an enzyme that produce hydrogen peroxide. Clin Chem. 28:2077–2080. 1982.PubMed/NCBI
13.	Izzo C, Grillo F and Murador E: Improved method for determination of high-density-lipoprotein cholesterol I. Isolation of high-density lipoproteins by use of polyethylene glycol 6000. Clin Chem. 27:371–374. 1981.PubMed/NCBI
14.	Kerscher L, Schiefer S, Draeger B, et al: Precipitation methods for the determination of LDL-cholesterol. Clin Biochem. 18:118–125. 1985. View Article : Google Scholar : PubMed/NCBI
15.	Xie WD, Wang H, Zhang JF, et al: Proteomic profile of visceral adipose tissues between low-fat diet-fed obesity-resistant and obesity-prone C57BL/6 mice. Mol Med Rep. 3:1047–1052. 2010.PubMed/NCBI
16.	Guo Y, Jolly RA, Halstead BW, et al: Underlying mechanisms of pharmacology and toxicity of a novel PPAR agonist revealed using rodent and canine hepatocytes. Toxicol Sci. 96:294–309. 2007. View Article : Google Scholar : PubMed/NCBI
17.	Pagliassotti MJ, Knobel SM, Shahrokhi KA, et al: Time course of adaptation to a high-fat diet in obesity-resistant and obesity-prone rats. Am J Physiol. 267:R659–R664. 1994.PubMed/NCBI
18.	Xie WD, Zhang YO, Wang NL, et al: Novel effects of macrostemonoside A, a compound from Allium macrostemon Bung, on hyperglycemia, hyperlipidemia, and visceral obesity in high-fat diet-fed C57BL/6 mice. Eur J Pharmacol. 599:159–165. 2008.PubMed/NCBI
19.	Trayhurn P and Beattie JH: Physiological role of adipose tissue: white adipose tissue as an endocrine and secretory organ. Proc Nutr Soc. 60:329–339. 2001. View Article : Google Scholar : PubMed/NCBI
20.	Rosicka M, Krsek M, Matoulek M, et al: Serum ghrelin levels in obese patients: the relationship to serum leptin levels and soluble leptin receptor levels. Physiol Res. 52:61–66. 2003.PubMed/NCBI
21.	Luo JD, Zhang GS and Chen MS: Leptin and cardiovascular diseases. Timely Top Med Cardiovasc Dis. 9:E342005.PubMed/NCBI
22.	Kannel WB and Vasan RS: Triglycerides as vascular risk factors: new epidemiologic insights. Curr Opin Cardiol. 24:345–350. 2009. View Article : Google Scholar : PubMed/NCBI
23.	Swinburn B, Sacks G and Ravussin E: Increased food energy supply is more than sufficient to explain the US epidemic of obesity. Am J Clin Nutr. 90:1453–1456. 2009. View Article : Google Scholar : PubMed/NCBI
24.	Kaplan LM: Pharmacologic therapies for obesity. Gastroenterol Clin North Am. 39:69–79. 2010. View Article : Google Scholar
25.	Huzarewich RL, Siemens CG and Booth SA: Application of “omics” to prion biomarker discovery. J Biomed Biotechnol. 2010:6135042010.
26.	Halvorsen YD, Wilkison WO and Briggs MR: Human adipocyte proteomics – a complementary way of looking at fat. Pharmacogenomics. 1:179–185. 2000.
27.	FitzPatrick DR, Germain-Lee E and Valle D: Isolation and characterization of rat and human cDNAs encoding a novel putative peroxisomal enoyl-CoA hydratase. Genomics. 27:457–466. 1995. View Article : Google Scholar : PubMed/NCBI
28.	Aoyama T, Peters JM, Iritani N, et al: Altered constitutive expression of fatty acid-metabolizing enzymes in mice lacking the peroxisome proliferator-activated receptor alpha (PPARalpha). J Biol Chem. 273:5678–5684. 1998. View Article : Google Scholar
29.	Xie WD, Nie Y, Du LJ, et al: Preventive effects of fenofibrate on insulin resistance, hyperglycaemia, visceral fat accumulation in NIH mice induced by small-dose streptozotocin and lard. Pharmacol Res. 55:392–399. 2007. View Article : Google Scholar : PubMed/NCBI
30.	Abbott BD: Review of the expression of peroxisome proliferator-activated receptors alpha (PPAR alpha), beta (PPAR beta), and gamma (PPAR gamma) in rodent and human development. Reprod Toxicol. 27:246–257. 2009. View Article : Google Scholar : PubMed/NCBI
31.	Miyazaki M, Nakagawa I, Koga S, et al: Proteomics analysis of cardiac muscle from rats with peroxisomal proliferator-activated receptor alpha (PPARalpha) stimulation. J Toxicol Sci. 35:131–135. 2010. View Article : Google Scholar
32.	Tsai YS and Maeda N: PPARgamma: a critical determinant of body fat distribution in humans and mice. Trends Cardiovasc Med. 15:81–85. 2005. View Article : Google Scholar : PubMed/NCBI