Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
February 2012 Volume 3 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February 2012 Volume 3 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Knockdown of vascular endothelial cell growth factor expression sensitizes U251 glioma cells to liposomal paclitaxel and radiation treatment in vitro

  • Authors:
    • Yang Yu
    • Jianguo Feng
    • Xiangyun Zong
    • Hongjian Yang
    • Dehong Zou
    • Xiangming He
  • View Affiliations / Copyright

    Affiliations: Department of Surgical Oncology, Zhejiang Cancer Hospital, Hangzhou, P.R. China
  • Pages: 181-186
    |
    Published online on: November 11, 2011
       https://doi.org/10.3892/etm.2011.379
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Glioblastoma is the most aggressive malignancy of the human brain, accounting for 40% of all primary malignant brain tumors. However, there is no effective treatment for this disease. This study was designed to develop anti-vascular endothelial growth factor (VEGF) as a novel adjuvant therapy for glioblastoma. A VEGF shRNA vector was constructed to silence VEGF expression in U251 glioma cells and these cells were treated with various concentrations of liposomal paclitaxel, 6 Gy radiation or liposomal paclitaxel plus radiation. The data demonstrated that the VEGF shRNA vector significantly knocked down VEGF expression, which synergistically sensitized U251 glioma cells to liposomal paclitaxel, radiation or liposomal paclitaxel plus radiation treatment in terms of cell viability, apoptosis, colony formation and morphological changes. Future studies are required to evaluate these effects in vivo.
View Figures

Figure 1

Figure 2

Figure 3

View References

1. 

A JemalT MurrayE WardCancer statisticsCA Cancer J Clin5510302005

2. 

A BehinK Hoang-XuanAF CarpentierPrimary brain tumours in adultsLancet361323331200310.1016/S0140-6736(03)12328-812559880

3. 

MA MeyerMalignant gliomas in adultsN Engl J Med35918502007

4. 

FB FurnariT FentonRM BachooMalignant astrocytic glioma: genetics, biology, and paths to treatmentGenes Dev2126832710200710.1101/gad.159670717974913

5. 

PC De Witt HamerSmall molecule kinase inhibitors in glioblastoma: a systematic review of clinical studiesNeuro Oncol12304316201020167819

6. 

HW LoEGFR-targeted therapy in malignant glioma: novel aspects and mechanisms of drug resistanceCurr Mol Pharmacol33752201010.2174/187446721100301003720030624

7. 

M MacheinLS de MiguelAngiogenesis in gliomasRecent Results Cancer Res171193215200910.1007/978-3-540-31206-2_12

8. 

RK JainE di TomasoDG DudaAngiogenesis in brain tumoursNat Rev Neurosci8610622200710.1038/nrn2175

9. 

MI LinWC SessaAntiangiogenic therapy: creating a unique ‘window’ of opportunityCancer Cell65295312004

10. 

HM SaidC HagemannA StaabExpression patterns of the hypoxia-related genes osteopontin, CA9, erythropoietin, VEGF and HIF-1alpha in human glioma in vitro and in vivoRadiother Oncol83398405200710.1016/j.radonc.2007.05.00317524506

11. 

V DamianoD MelisiC BiancoCooperative antitumor effect of multitargeted kinase inhibitor ZD6474 and ionizing radiation in glioblastomaClin Cancer Res1156395644200510.1158/1078-0432.CCR-05-017416061883

12. 

A AbdollahiKE LipsonA SckellCombined therapy with direct and indirect angiogenesis inhibition results in enhanced anti-angiogenic and antitumor effectsCancer Res6388908898200314695206

13. 

WS KamounCD LeyCT FarrarEdema control by cediranib, a vascular endothelial growth factor receptor-targeted kinase inhibitor, prolongs survival despite persistent brain tumor growth in miceJ Clin Oncol2725422552200910.1200/JCO.2008.19.9356

14. 

G TabatabaiB FrankA WickSynergistic antiglioma activity of radiotherapy and enzastaurinAnn Neurol61153161200710.1002/ana.2105717212356

15. 

PR WachsbergerR BurdC CardiVEGF trap in combination with radiotherapy improves tumor control in u87 glioblastomaInt J Radiat Oncol Biol Phys6715261537200710.1016/j.ijrobp.2006.11.01117234361

16. 

F WinklerSV KozinRT TongKinetics of vascular normalization by VEGFR2 blockade governs brain tumor response to radiation: role of oxygenation, angiopoietin-1, and matrix metalloproteinasesCancer Cell65535632004

17. 

KE HovingaLJ StalpersC van BreeRadiation-enhanced vascular endothelial growth factor (VEGF) secretion in glioblastoma multiforme cell lines - a clue to radioresistance?J Neurooncol7499103200510.1007/s11060-004-4204-716193379

18. 

G FountzilasG KarkavelasA Kalogera-FountzilaPost-operative combined radiation and chemotherapy with temozolomide and irinotecan in patients with high-grade astrocytic tumors. A phase II study with biomarker evaluationAnticancer Res2646754686200617214326

19. 

S KesariD SchiffJW HensonPhase II study of temozolomide, thalidomide, and celecoxib for newly diagnosed glioblastoma in adultsNeuro Oncol10300308200810.1215/15228517-2008-00518403492

20. 

Y MatsumuraA MaedaA new concept for macromolecular therapies in cancer chemotherapy: mechanisms of tumortropic accumulation of proteins and the antitumor agents smancsCancer Res466387639219862946403

21. 

KJ HarringtonS MohammadtaghiPS UsterEffective targeting of solid tumors in patients with locally advanced cancers by radiolabeled pegylated liposomesClin Cancer Res7243254200111234875

22. 

CO NobleDB KirpotinME HayesDevelopment of ligand targeted liposomes for cancer therapyExpert Opin Ther Targets8335353200410.1517/14728222.8.4.33515268628

23. 

H AokiK KakinumaK MoritaTherapeutic efficacy of targeting chemotherapy using local hyperthermia and thermosensitive liposome: evaluation of drug distribution in a rat glioma modelInt J Hypertherm20595605200510.1080/0265673041000170318615370816

24. 

C MamotJB NguyenM PourdehnadExtensive distribution of liposomes in rodent brains and brain tumors following convection-enhanced deliveryJ Neurooncol6819200410.1023/B:NEON.0000024743.56415.4b15174514

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yu Y, Feng J, Zong X, Yang H, Zou D and He X: Knockdown of vascular endothelial cell growth factor expression sensitizes U251 glioma cells to liposomal paclitaxel and radiation treatment in vitro. Exp Ther Med 3: 181-186, 2012.
APA
Yu, Y., Feng, J., Zong, X., Yang, H., Zou, D., & He, X. (2012). Knockdown of vascular endothelial cell growth factor expression sensitizes U251 glioma cells to liposomal paclitaxel and radiation treatment in vitro. Experimental and Therapeutic Medicine, 3, 181-186. https://doi.org/10.3892/etm.2011.379
MLA
Yu, Y., Feng, J., Zong, X., Yang, H., Zou, D., He, X."Knockdown of vascular endothelial cell growth factor expression sensitizes U251 glioma cells to liposomal paclitaxel and radiation treatment in vitro". Experimental and Therapeutic Medicine 3.2 (2012): 181-186.
Chicago
Yu, Y., Feng, J., Zong, X., Yang, H., Zou, D., He, X."Knockdown of vascular endothelial cell growth factor expression sensitizes U251 glioma cells to liposomal paclitaxel and radiation treatment in vitro". Experimental and Therapeutic Medicine 3, no. 2 (2012): 181-186. https://doi.org/10.3892/etm.2011.379
Copy and paste a formatted citation
x
Spandidos Publications style
Yu Y, Feng J, Zong X, Yang H, Zou D and He X: Knockdown of vascular endothelial cell growth factor expression sensitizes U251 glioma cells to liposomal paclitaxel and radiation treatment in vitro. Exp Ther Med 3: 181-186, 2012.
APA
Yu, Y., Feng, J., Zong, X., Yang, H., Zou, D., & He, X. (2012). Knockdown of vascular endothelial cell growth factor expression sensitizes U251 glioma cells to liposomal paclitaxel and radiation treatment in vitro. Experimental and Therapeutic Medicine, 3, 181-186. https://doi.org/10.3892/etm.2011.379
MLA
Yu, Y., Feng, J., Zong, X., Yang, H., Zou, D., He, X."Knockdown of vascular endothelial cell growth factor expression sensitizes U251 glioma cells to liposomal paclitaxel and radiation treatment in vitro". Experimental and Therapeutic Medicine 3.2 (2012): 181-186.
Chicago
Yu, Y., Feng, J., Zong, X., Yang, H., Zou, D., He, X."Knockdown of vascular endothelial cell growth factor expression sensitizes U251 glioma cells to liposomal paclitaxel and radiation treatment in vitro". Experimental and Therapeutic Medicine 3, no. 2 (2012): 181-186. https://doi.org/10.3892/etm.2011.379
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team