Glycan-related gene expression signatures in human metastatic hepatocellular carcinoma cells

  • Authors:
    • Xiaonan Kang
    • Nian Wang
    • Chen Pei
    • Lu Sun
    • Ruixia Sun
    • Jie Chen
    • Yinkun Liu
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  • Published online on: December 23, 2011     https://doi.org/10.3892/etm.2011.430
  • Pages: 415-422
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Abstract

Human hepatocellular carcinoma (HCC) ranks second in cancer mortality in China; recurrence and metastasis have been the cause of the high mortality. Glycans on the cell surface play a pivotal role in tumor metastasis. The global alteration in the structure and composition of N-glycans during HCC metastasis remains unknown. To understand glycan alterations of glycoproteins by correlating the glycosyltransferase expression profile with glycan structure, we systematically used glycan profiling tools: glycogene microarray analyses of 115 genes, including glycotransferases, glycosidases and nuclear sugar transporters and lectin chips to investigate the glycan-related gene expression signatures in the high metastatic potential HCC cell line, HCCLM3, in comparison to the HCC cell line, Hep3B, with low metastatic potential. Of the 115 genes, 18 genes were up‑regulated in high metastatic potential HCCLM3 cells in comparison to Hep3B cells, while 11 genes were down-regulated. The differentially expressed genes, such as ST3GalI, FUT8, β3GalT5, MGAT3 and MGAT5, were mainly involved in the synthesis of N-glycan and glycolipids, particularly the sialyl Lewis antigen. The results of the glycogene microarray analysis were further validated by quantitative real-time PCR analysis and lectin-based analysis. The differentially expressed glycogenes identified in this study may provide new insights and represent novel factors for investigating the functional role of cell surface carbohydrate‑mediated HCC metastasis.
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March 2012
Volume 3 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Kang X, Wang N, Pei C, Sun L, Sun R, Chen J and Liu Y: Glycan-related gene expression signatures in human metastatic hepatocellular carcinoma cells. Exp Ther Med 3: 415-422, 2012.
APA
Kang, X., Wang, N., Pei, C., Sun, L., Sun, R., Chen, J., & Liu, Y. (2012). Glycan-related gene expression signatures in human metastatic hepatocellular carcinoma cells. Experimental and Therapeutic Medicine, 3, 415-422. https://doi.org/10.3892/etm.2011.430
MLA
Kang, X., Wang, N., Pei, C., Sun, L., Sun, R., Chen, J., Liu, Y."Glycan-related gene expression signatures in human metastatic hepatocellular carcinoma cells". Experimental and Therapeutic Medicine 3.3 (2012): 415-422.
Chicago
Kang, X., Wang, N., Pei, C., Sun, L., Sun, R., Chen, J., Liu, Y."Glycan-related gene expression signatures in human metastatic hepatocellular carcinoma cells". Experimental and Therapeutic Medicine 3, no. 3 (2012): 415-422. https://doi.org/10.3892/etm.2011.430