microRNA-192, -194 and -215 are frequently downregulated in colorectal cancer

Chiang,Yeunpo; Song,Yongxi; Wang,Zhenning; Liu,Zhuangkai; Gao,Peng; Liang,Jiwang; Zhu,Jinliang; Xing,Chengzhong; Xu,Huimian

doi:10.3892/etm.2011.436

March 2012 Volume 3 Issue 3

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March 2012 Volume 3 Issue 3

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Article

microRNA-192, -194 and -215 are frequently downregulated in colorectal cancer

Authors:
- Yeunpo Chiang
- Yongxi Song
- Zhenning Wang
- Zhuangkai Liu
- Peng Gao
- Jiwang Liang
- Jinliang Zhu
- Chengzhong Xing
- Huimian Xu
View Affiliations / Copyright

Affiliations: Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, Shenyang 110001, P.R. China
Pages: 560-566
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Published online on: December 28, 2011

https://doi.org/10.3892/etm.2011.436
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Abstract

microRNAs (miRNAs) are small, non-coding RNAs of endogenous origin. They have been increasingly shown to have aberrant expression in a number of tumor types. miR-192, -194 and -215 have not been comprehensively investigated using a large number of cases in colorectal cancer (CRC). We extracted total RNA from 107 CRC tissues and three CRC cell lines. Following polyadenylation and reverse transcription, the expression levels of miR-192, -194 and -215 were determined for evaluation of the association between expression levels and clinicopathological characteristics by a quantitative real-time polymerase chain reaction (real-time PCR) method. Finally, we studied the impact of miR-194 on cell proliferation in HCT-116 cells by MTT assay. miR-192, -194 and -215 were significantly downregulated in CRC tissues (all p<0.001, paired t-test) and cancer cell lines (all p<0.05) compared to non-tumor counterparts. Moreover, the expression levels of miR-192, -194 and -215 were demonstrated to be associated with increased tumor sizes (p=0.027, p=0.018, and p=0.027, respectively; Mann-Whitney U test). Also, there were marked correlations among these miRNAs in CRC tissues (all p<0.001, Pearson's regression analysis). Furthermore, we found that the overexpression of miR-194 could significantly inhibit cell proliferation in HTC-116 cells. miR-192, -194 and -215 may be important biological markers as tumor suppressors in the carcinogenesis of CRC.

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1.	DP BartelMicroRNAs: genomics, biogenesis, mechanism, and functionCell116281297200410.1016/S0092-8674(04)00045-514744438
2.	GA CalinCM CroceMicroRNA-cancer connection: the beginning of a new taleCancer Res6673907394200610.1158/0008-5472.CAN-06-080016885332
3.	M LiJ LiX DingM HeSY ChengmicroRNA and cancerAAPS J12309317201010.1208/s12248-010-9194-0
4.	WC ChoOncomiRs: the discovery and progress of microRNAs in cancersMol Cancer660200710.1186/1476-4598-6-6017894887
5.	GA CalinCM CroceMicroRNA signatures in human cancerNat Rev Cancer6857866200610.1038/nrc199717060945
6.	EA MathéGH NguyenED BowmanMicroRNA expression in squamous cell carcinoma and adenocarcinoma of the esophagus: associations with survivalClin Cancer Res1561926200200919789312
7.	N YanaiharaN CaplenE BowmanUnique microRNA molecular profiles in lung cancer diagnosis and prognosisCancer Cell9189198200610.1016/j.ccr.2006.01.02516530703
8.	J GuiY TianX WenSerum microRNA characterization identifies miR-885-5p as a potential marker for detecting liver pathologiesClin Sci120183193201110.1042/CS2010029720815808
9.	ST MeesWA MardinC WendelEP300 - a miRNA-regulated metastasis suppressor gene in ductal adenocarcinomas of the pancreasInt J Cancer126114124201010.1002/ijc.2469519569050
10.	F GottardoCG LiuM FerracinMicro-RNA profiling in kidney and bladder cancersUrol Oncol25387392200710.1016/j.urolonc.2007.01.01917826655
11.	MV IorioR VisoneG Di LevaMicroRNA signatures in human ovarian cancerCancer Res6786998707200710.1158/0008-5472.CAN-07-193617875710
12.	YX SongZY YueZN WangMicroRNA-148b is frequently down-regulated in gastric cancer and acts as a tumor suppressor by inhibiting cell proliferationMol Cancer101201110.1186/1476-4598-10-121205300
13.	JM CumminsY HeRJ LearyThe colorectal microRNAomeProc Natl Acad Sci USA10336873692200610.1073/pnas.051115510316505370
14.	Y ChenY SongZ WangZ YueH XuC XingZ LiuAltered expression of miR-148a and miR-152 in gastrointestinal cancers and its clinical significanceJ Gastrointest Surg1411701179201010.1007/s11605-010-1202-220422307
15.	Y ChiangY SongZ WangAberrant expression of miR-203 and its clinical significance in gastric and colorectal cancersJ Gastrointest Surg156367201110.1007/s11605-010-1367-821063914
16.	SA GeorgesMC BierySY KimCoordinated regulation of cell cycle transcripts by p53-Inducible microRNAs, miR-192 and miR-215Cancer Res681010510112200810.1158/0008-5472.CAN-08-184619074876
17.	R ShiVL ChiangFacile means for quantifying microRNA expression by real-time PCRBiotechniques39519525200510.2144/00011201016235564
18.	KJ LivakTD SchmittgenAnalysis of relative gene expression data using real-time quantitative pcr and the 2 (delta delta c(T)) methodMethods25402408200110.1006/meth.2001.126211846609
19.	LP LimNC LauP Garrett-EngeleMicroarray analysis shows that some microRNAs downregulate large numbers of target mRNAsNature433769773200510.1038/nature0331515685193
20.	S LuanL SunF HuangMicroRNA-34a: a novel tumor suppressor in p53-mutant glioma cell line U251Arch Med Res416774201010.1016/j.arcmed.2010.02.00720470934
21.	CJ WangZG ZhouL WangClinicopathological significance of microRNA-31, -143 and -145 expression in colorectal cancerDis Markers262734200910.1155/2009/92190719242066
22.	A Esquela-KerscherFJ SlackOncomirs - microRNAs with a role in cancerNat Rev Cancer6259269200610.1038/nrc1840
23.	Z JinFM SelaruY ChengMicroRNA-192 and -215 are upregulated in human gastric cancer in vivo and suppress ALCAM expression in vitroOncogene3015771585201110.1038/onc.2010.53421119604
24.	Z MengX FuX ChenmiR-194 is a marker of hepatic epithelial cells and suppresses metastasis of liver cancer cells in miceHepatology5221482157201010.1002/hep.2391520979124
25.	R BaffaM FassanS VoliniaMicroRNA expression profiling of human metastatic cancers identifies cancer gene targetsJ Pathol219214221200910.1002/path.258619593777
26.	CJ BraunX ZhangI Savelyevap53-Responsive micrornas 192 and 215 are capable of inducing cell cycle arrestCancer Res681009410104200810.1158/0008-5472.CAN-08-156919074875
27.	LA DavidsonN WangMS ShahJR LuptonI IvanovRS Chapkinn-3 Polyunsaturated fatty acids modulate carcinogen-directed non-coding microRNA signatures in rat colonCarcinogenesis3020772084200910.1093/carcin/bgp24519825969
28.	SR HamiltonLA AaltonenPathology and genetics of tumours of the digestive systemWorld Health Organization Classification of TumoursP KleihuesvLH SobinIARC PressLyon1031442000
29.	V BoniN BitarteI CristobalmiR-192/miR-215 influence 5-fluorouracil resistance through cell cycle-mediated mechanisms complementary to its post-transcriptional thymidilate synthase regulationMol Cancer Ther922652275201010.1158/1535-7163.MCT-10-0061
30.	B SongY WangMA TitmusG BotchkinaA FormentiniM KornmannJ JuMolecular mechanism of chemoresistance by miR-215 in osteosarcoma and colon cancer cellsMol Cancer996201010.1186/1476-4598-9-9620433742
31.	B SongY WangK KudoEJ GavinY XiJ JumiR-192 Regulates dihydrofolate reductase and cellular proliferation through the p53-microRNA circuitClin Cancer Res1480808086200810.1158/1078-0432.CCR-08-1422
32.	F PichiorriSS SuhA RocciDownregulation of p53-inducible microRNAs 192, 194, and 215 impairs the p53/ MDM2 autoregulatory loop in multiple myeloma developmentCancer Cell18367381201010.1016/j.ccr.2010.09.00520951946
33.	K HinoK TsuchiyaT FukaoK KigaR OkamotoT KanaiM WatanabeInducible expression of microRNA-194 is regulated by HNF-1alpha during intestinal epithelial cell differentiationRNA1414331442200810.1261/rna.81020818492795

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

microRNA-192, -194 and -215 are frequently downregulated in colorectal cancer

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