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Metabonomic signature analysis of cervical carcinoma and precancerous lesions in women by 1H NMR spectroscopy

  • Authors:
    • Ayshamgul Hasim
    • Mayinuer Ali
    • Batur Mamtimin
    • Jun‑Qi  Ma
    • Qiao-Zhi Li
    • Abulizi Abudula
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, College of Basic Medicine, Medical University of Xinjiang, Urumqi, P.R. China, Department of Oncology, The First Affiliated Hospital, Medical University of Xinjiang, Urumqi, P.R. China, Centers of Pharmaceutical Analysis, College of Pharmacy, Medical University of Xinjiang, Urumqi, P.R. China, Department of Gynecology, The First Affiliated Hospital, Medical University of Xinjiang, Urumqi, P.R. China, Key Laboratories of Molecular Biology and Endemic Diseases, Medical University of Xinjiang, Urumqi, P.R. China
  • Pages: 945-951
    |
    Published online on: March 12, 2012
       https://doi.org/10.3892/etm.2012.509
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Abstract

1H nuclear magnetic resonance (NMR)-based metabonomics has been used to characterize the metabolic profiles of cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (CSCC). Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) were used to model the systematic variation related to patients with CIN or CSCC with healthy controls. Potential metabolic biomarkers were identified using database comparisons, and the one‑way analysis of variance (ANOVA) test was used to examine the significance of the metabolites. Compared with plasma obtained from the healthy controls, plasma from patients with CIN had higher levels of very-low density lipoprotein (VLDL), acetone, unsaturated lipid and carnitine, together with lower levels of creatine, lactate, isoleucine, leucine, valine, alanine, glutamine, histidine, glycine, acetylcysteine, myo-inositol, choline and glycoprotein. Plasma from patients with CSCC had higher levels of acetate and formate, together with lower levels of creatine, lactate, isoleucine, leucine, valine, alanine, glutamine, histidine and tyrosine compared with the plasma of the healthy controls. In addition, compared with the plasma of patients with CIN, the plasma of CSCC patients had higher levels of acetate, formate, lactate, isoleucine, leucine, valine, alanine, glutamine, histidine, tyrosine, acetylcysteine, myo‑inositol, glycoprotein, α-glucose and β-glucose, together with lower levels of acetone, unsaturated lipid and carnitine. Moreover, the profiles showed high feasibility and specificity by statistical analysis with OPLS-DA compared to the Thinprep cytology test (TCT) by setting the histopathological outcome as standard. The metabolic profile obtained for cervical cancer is significant, even for the precancerous disease. This suggests a systemic metabolic response to cancer, which may be used to identify potential early diagnostic biomarkers of the cancer and to establish clinical diagnostic methods.
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Copy and paste a formatted citation
Spandidos Publications style
Hasim A, Ali M, Mamtimin B, Ma JQ, Li Q and Abudula A: Metabonomic signature analysis of cervical carcinoma and precancerous lesions in women by 1H NMR spectroscopy. Exp Ther Med 3: 945-951, 2012.
APA
Hasim, A., Ali, M., Mamtimin, B., Ma, J., Li, Q., & Abudula, A. (2012). Metabonomic signature analysis of cervical carcinoma and precancerous lesions in women by 1H NMR spectroscopy. Experimental and Therapeutic Medicine, 3, 945-951. https://doi.org/10.3892/etm.2012.509
MLA
Hasim, A., Ali, M., Mamtimin, B., Ma, J., Li, Q., Abudula, A."Metabonomic signature analysis of cervical carcinoma and precancerous lesions in women by 1H NMR spectroscopy". Experimental and Therapeutic Medicine 3.6 (2012): 945-951.
Chicago
Hasim, A., Ali, M., Mamtimin, B., Ma, J., Li, Q., Abudula, A."Metabonomic signature analysis of cervical carcinoma and precancerous lesions in women by 1H NMR spectroscopy". Experimental and Therapeutic Medicine 3, no. 6 (2012): 945-951. https://doi.org/10.3892/etm.2012.509
Copy and paste a formatted citation
x
Spandidos Publications style
Hasim A, Ali M, Mamtimin B, Ma JQ, Li Q and Abudula A: Metabonomic signature analysis of cervical carcinoma and precancerous lesions in women by 1H NMR spectroscopy. Exp Ther Med 3: 945-951, 2012.
APA
Hasim, A., Ali, M., Mamtimin, B., Ma, J., Li, Q., & Abudula, A. (2012). Metabonomic signature analysis of cervical carcinoma and precancerous lesions in women by 1H NMR spectroscopy. Experimental and Therapeutic Medicine, 3, 945-951. https://doi.org/10.3892/etm.2012.509
MLA
Hasim, A., Ali, M., Mamtimin, B., Ma, J., Li, Q., Abudula, A."Metabonomic signature analysis of cervical carcinoma and precancerous lesions in women by 1H NMR spectroscopy". Experimental and Therapeutic Medicine 3.6 (2012): 945-951.
Chicago
Hasim, A., Ali, M., Mamtimin, B., Ma, J., Li, Q., Abudula, A."Metabonomic signature analysis of cervical carcinoma and precancerous lesions in women by 1H NMR spectroscopy". Experimental and Therapeutic Medicine 3, no. 6 (2012): 945-951. https://doi.org/10.3892/etm.2012.509
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