Expression and subcellular localization of menin in human cancer cells

Ren,Feng; Xu,Hong-Wei; Hu,Yu; Yan,Shuang-Hong; Wang,Feng; Su,Bao-Wei; Zhao,Qi

doi:10.3892/etm.2012.530

June 2012 Volume 3 Issue 6

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June 2012 Volume 3 Issue 6

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Article

Expression and subcellular localization of menin in human cancer cells

Authors:
- Feng Ren
- Hong-Wei Xu
- Yu Hu
- Shuang-Hong Yan
- Feng Wang
- Bao-Wei Su
- Qi Zhao
View Affiliations / Copyright

Affiliations: Department of Clinical Laboratory, The Fourth People's Hospital of Wuxi, Wuxi, Jiangsu 214062, P.R. China, Department of General Surgery, The Fourth People's Hospital of Wuxi, Wuxi, Jiangsu 214062, P.R. China
Pages: 1087-1091
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Published online on: March 29, 2012

https://doi.org/10.3892/etm.2012.530
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Abstract

The aim of this study was to elucidate the expression and localization of menin, a protein encoded by the multiple endocrine neoplasia type I (MEN1) gene, in 13 human cancer cell lines. Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the expression of the menin gene. The localization of the menin protein was detected by immunofluorescence microscopy. Western blotting was used to determine the quantity of menin in the nucleus, cytosol and membrane of the cells. RT-PCR revealed that menin was expressed in all the cell lines examined in this study. Immunofluorescence microscopy revealed that menin was located primarily in the nucleus. In the GES-1 (transformed human gastric epithelium), MCF-7 (breast cancer), SGH44 (brain glioma) and HeLa (cervical cancer) cell lines, menin was also found to be localized to the membrane, cytosol and nucleus. Moreover, in SGH44 cells more menin was located in the cytosol than the nucleus. Similar findings were obtained by western blotting. In the GES-1 and MKN-28 cells undergoing octreotide treatment, cytoplasmic menin was significantly increased compared with the control groups. Therefore, we suggest that menin is expressed in a number of human cancer cell lines and that the cytosolic distribution increases when the cells undergo octreotide treatment, indicating a new role for menin.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Expression and subcellular localization of menin in human cancer cells

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Abstract

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