A potential role for macrophages in maintaining lipopolysaccharide-induced subacute airway inflammation in rats

Liu,Lin; Chen,Lei; Wang,Yongsheng; Yang,Hua; Chen,Yifang; Xu,Xiaoya; Zhou,Hang; Jiang,Fangping; Li,Tonglin; Wang,Junli

doi:10.3892/etm.2012.726

December 2012 Volume 4 Issue 6

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

December 2012 Volume 4 Issue 6

Full Size Image

Article

A potential role for macrophages in maintaining lipopolysaccharide-induced subacute airway inflammation in rats

Authors:
- Lin Liu
- Lei Chen
- Yongsheng Wang
- Hua Yang
- Yifang Chen
- Xiaoya Xu
- Hang Zhou
- Fangping Jiang
- Tonglin Li
- Junli Wang
View Affiliations / Copyright

Affiliations: Department of Respiratory Medicine, 363 Hospital, Chengdu, Sichuan, 610041, P.R. China, Department of Respiratory Medicine, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Cardiology, 363 Hospital, Chengdu, Sichuan 610041, P.R. China
Pages: 983-986
|
Published online on: September 27, 2012

https://doi.org/10.3892/etm.2012.726
Expand metrics +

Abstract

Bacterial infection is a key factor in airway inflammation. The present study describes the time‑dependent changes in the leukocyte counts and cytokine levels of the bronchoalveolar lavage fluid (BALF) following subacute airway inflammation induced by lipopolysaccharide (LPS), a major component of the outer membranes of Gram‑negative bacteria. LPS (200 µg/rat) or saline was intratracheally administered to rats which were sacrificed 2, 4 or 7 days after LPS treatment. Airway inflammation was evaluated using hematoxylin and eosin staining, cell counts and proinflammatory cytokine levels in the BALF. Rat airways obtained from the LPS group exhibited marked airway wall thickening and infiltration of inflammatory cells compared with the control group, as well as elevated cell counts (neutrophils, macrophages, lymphocytes) and proinflammatory cytokine levels [(tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β, cytokine-induced neutrophil chemoattractant (CINC)‑1)] in the BALF, which peaked on day 2 and subsequently decreased until the experimental endpoint. Notably, IL‑1β levels induced by LPS changed in a similar manner to macrophage cell counts, but not neutrophil and lymphocyte counts. Moreover, TNF‑α and CINC‑1 levels did not decrease as rapidly as neutrophil counts after peaking. These findings suggest that macrophages may play a significant role in maintaining subacute inflammatory responses induced by LPS in rat airways.

View Figures

View References

1	Toews GB: Impact of bacterial infections on airway diseases. Eur Respir Rev. 14:62–68. 2005. View Article : Google Scholar
2	Bishop RE: Fundamentals of endotoxin structure and function. Contrib Microbiol. 12:1–27. 2005. View Article : Google Scholar : PubMed/NCBI
3	Brass DM, Hollingsworth JW, McElvania-Tekippe E, Garantziotis S, Hossain I and Schwartz DA: CD14 is an essential mediator of LPS-induced airway disease. Am J Physiol Lung Cell Mol Physiol. 293:L77–L83. 2007. View Article : Google Scholar : PubMed/NCBI
4	Chen L, Wang T, Zhang JY, Zhang SF, Liu DS, Xu D, Wang X, Chen YJ and Wen FQ: Toll-like receptor 4 relates to lipopolysaccharide-induced mucus hypersecretion in rat airway. Arch Med Res. 40:10–17. 2009. View Article : Google Scholar : PubMed/NCBI
5	Cimolai N, Taylor GP, Mah D and Morrison BJ: Definition and application of a histopathological scoring scheme for an animal model of acute Mycoplasma pneumoniae pulmonary infection. Microbiol Immunol. 36:465–478. 1992. View Article : Google Scholar : PubMed/NCBI
6	Dinarello CA: Proinflammatory cytokines. Chest. 118:503–508. 2000. View Article : Google Scholar
7	Peters-Golden M: The alveolar macrophage: the forgotten cell in asthma. Am J Respir Cell Mol Biol. 31:3–7. 2004. View Article : Google Scholar : PubMed/NCBI
8	Mangan DF and Wahl SM: Differential regulation of human monocyte programmed cell death (apoptosis) by chemotactic factors and pro-inflammatory cytokines. J Immunol. 147:3408–3412. 1991.PubMed/NCBI
9	Welgus HG, Campbell EJ, Bar-Shavit Z, Senior RM and Teitelbaum SL: Human alveolar macrophages produce a fibroblast-like collagenase and collagenase inhibitor. J Clin Invest. 76:219–224. 1985. View Article : Google Scholar : PubMed/NCBI
10	Linden A and Adachi M: Neutrophilic airway inflammation and IL-17. Allergy. 57:769–775. 2002. View Article : Google Scholar : PubMed/NCBI
11	Moreland JG, Fuhrman RM, Wohlford-Lenane CL, Quinn TJ, Benda E, Pruessner JA and Schwartz DA: TNF-alpha and IL-1 beta are not essential to the inflammatory response in LPS-induced airway disease. Am J Physiol Lung Cell Mol Physiol. 280:L173–L180. 2001.PubMed/NCBI
12	Mukaida N: Pathophysiological roles of interleukin-8/CXCL8 in pulmonary diseases. Am J Physiol Lung Cell Mol Physiol. 284:L566–L577. 2003. View Article : Google Scholar : PubMed/NCBI
13	Kuwahara I, Lillehoj EP, Lu W, Singh IS, Isohama Y, Miyata T and Kim KC: Neutrophil elastase induces IL-8 gene transcription and protein release through p38/NF-(kappa)B activation via EGFR transactivation in a lung epithelial cell line. Am J Physiol Lung Cell Mol Physiol. 291:L407–L416. 2006. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

A potential role for macrophages in maintaining lipopolysaccharide-induced subacute airway inflammation in rats

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Related Articles