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Article

Liquid nitrogen-cryopreserved tarsal plate and palpebral conjunctival transplantation for the repair of tarsal defects

  • Authors:
    • Yang Liu
    • Lin-Nong Wang
    • Li-Xun Chen
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210006, P.R. China
  • Pages: 1411-1417
    |
    Published online on: March 12, 2013
       https://doi.org/10.3892/etm.2013.1004
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Abstract

The objectives of this study were to compare the efficiency of three methods for repairing tarsal defects (glycerine-preserved, alcohol-preserved and cryopreserved tarsal plate and palpebral conjunctival transplantation) based on histopathological changes and apoptosis, and to evaluate the clinical effects of repairing tarsal defects by liquid nitrogen-cryopreserved tarsal plate and palpebral conjunctival transplantation. Forty-eight rabbit eyes with tarsal defects were equally divided into three groups and transplanted with tarsal plates and palpebral conjunctivae under the following conditions: Group 1, liquid nitrogen cryopreservation; Group 2, glycerine preservation; and Group 3, alcohol preservation. Histopathological changes in the transplants were observed by light microscopy 1 week, 1 month and 3 months postoperatively. Apoptosis was assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. Clinically, transplantations of liquid nitrogen-cryopreserved tarsal plates and palpebral conjunctivae were performed in 30 eyes (29 cases) to repair tarsal defects caused by the excision of neoplasms. The postoperative inflammatory reaction and number of apoptotic cells in Group 1 were lower compared with those in Groups 2 and 3. Clinically, of the 30 eyes operated on, 14 were cured, 15 improved and 1 failed between 6 and 90 months of follow-up. Liquid nitrogen-cryopreserved tarsal plate and palpebral conjunctival transplantation is an easy, feasible and convenient procedure. Its effects are fairly favorable, with only a small rejection rate postoperatively. Therefore, it is an ideal method for repairing tarsal defects.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Wang L and Chen L: Liquid nitrogen-cryopreserved tarsal plate and palpebral conjunctival transplantation for the repair of tarsal defects. Exp Ther Med 5: 1411-1417, 2013.
APA
Liu, Y., Wang, L., & Chen, L. (2013). Liquid nitrogen-cryopreserved tarsal plate and palpebral conjunctival transplantation for the repair of tarsal defects. Experimental and Therapeutic Medicine, 5, 1411-1417. https://doi.org/10.3892/etm.2013.1004
MLA
Liu, Y., Wang, L., Chen, L."Liquid nitrogen-cryopreserved tarsal plate and palpebral conjunctival transplantation for the repair of tarsal defects". Experimental and Therapeutic Medicine 5.5 (2013): 1411-1417.
Chicago
Liu, Y., Wang, L., Chen, L."Liquid nitrogen-cryopreserved tarsal plate and palpebral conjunctival transplantation for the repair of tarsal defects". Experimental and Therapeutic Medicine 5, no. 5 (2013): 1411-1417. https://doi.org/10.3892/etm.2013.1004
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Wang L and Chen L: Liquid nitrogen-cryopreserved tarsal plate and palpebral conjunctival transplantation for the repair of tarsal defects. Exp Ther Med 5: 1411-1417, 2013.
APA
Liu, Y., Wang, L., & Chen, L. (2013). Liquid nitrogen-cryopreserved tarsal plate and palpebral conjunctival transplantation for the repair of tarsal defects. Experimental and Therapeutic Medicine, 5, 1411-1417. https://doi.org/10.3892/etm.2013.1004
MLA
Liu, Y., Wang, L., Chen, L."Liquid nitrogen-cryopreserved tarsal plate and palpebral conjunctival transplantation for the repair of tarsal defects". Experimental and Therapeutic Medicine 5.5 (2013): 1411-1417.
Chicago
Liu, Y., Wang, L., Chen, L."Liquid nitrogen-cryopreserved tarsal plate and palpebral conjunctival transplantation for the repair of tarsal defects". Experimental and Therapeutic Medicine 5, no. 5 (2013): 1411-1417. https://doi.org/10.3892/etm.2013.1004
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