Comparison of treatments for bullous keratopathy in rabbits

Zhao,Haixia; Luo,Yunna; Niu,Chunmei; Guan,Wenying

doi:10.3892/etm.2013.1025

May 2013 Volume 5 Issue 5

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May 2013 Volume 5 Issue 5

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Article

Comparison of treatments for bullous keratopathy in rabbits

Authors:
- Haixia Zhao
- Yunna Luo
- Chunmei Niu
- Wenying Guan
View Affiliations / Copyright

Affiliations: Department of Ophthalmology, The Affiliated Hospital of Inner Mongolia Medical College, Hohhot, Inner Mongolia 010050, P.R. China
Pages: 1481-1485
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Published online on: March 21, 2013

https://doi.org/10.3892/etm.2013.1025
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Abstract

The aim of the present study was to compare deep lamellar endothelial keratoplasty (DLEK) and penetrating keratoplasty (PK) treatments for bullous keratopathy (BK). In total, 36 healthy New Zealand white rabbits were randomly divided into 3 groups termed the experimental, DLEK and PK groups. The experimental control group received no treatment. The DLEK and PK groups were observed for corneal astigmatism at 1, 2, or 3 months post‑surgery using a corneal topography instrument and a slit lamp microscope. The incidence of immune rejection after 3 months of recovery was determined using hematoxylin and eosin (H&E) staining. The corneal specimens from the surgery groups were compared with those from the control group. In the 12 rabbit eyes that underwent the DLEK surgery, the central cornea became clear after 1 week. After 3 months, these corneas were almost transparent and no eye infections or other complications were observed in 10 of the eyes, while surgical perforations in 2 eyes led to surgical lamellar failure. In the PK surgery group, in which 12 rabbit eyes were also treated, nine were almost transparent after 3 months of recovery, while three eyes were immunologically rejected due to the corneal grafts. The occurrences of corneal astigmatism that were observed following DLEK and PK treatment were significantly different after 1, 2 and 3 months of recovery (P<0.05). Normal corneal staining was observed in the DLEK and PK rabbits subjected to H&E staining after 3 months of recovery. A BK animal model was established by curetting the Descemet's membrane (DM film). In comparison with PK, DLEK is a superior surgical treatment for BK.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Comparison of treatments for bullous keratopathy in rabbits

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