Regulation of synoviocyte activity by resveratrol in rats with adjuvant arthritis

Chen,Xiao-Yu; Wang,Zhi-Cheng; Li,Jun; Liu,Xiao-Li; Sun,Yu-Hua

doi:10.3892/etm.2013.1078

Regulation of synoviocyte activity by resveratrol in rats with adjuvant arthritis

Authors:
- Xiao-Yu Chen
- Zhi-Cheng Wang
- Jun Li
- Xiao-Li Liu
- Yu-Hua Sun
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Affiliations: Department of Histology and Embryology, Anhui Medical University, Hefei, Anhui 230032, P.R. China, Laboratorial Center, Shanghai Municipal Affiliated Hospital of Shanghai University of TCM, Shanghai 200071, P.R. China, School of Pharmacy, Anhui Medical University, Hefei, Anhui 230032, P.R. China
Published online on: April 25, 2013 https://doi.org/10.3892/etm.2013.1078
Pages: 172-176

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Abstract

The aim of this study was to investigate the preventive effects of resveratrol (Res) on rats with adjuvant arthritis (AA) and the mechanism(s) of action. An AA model was established by injection of Freund's complete adjuvant (FCA). Vascular endothelial growth factor (VEGF) was visualized in joint specimens using immunohistochemistry. IL-1β and TNF-α production in synoviocytes was determined by radioimmunoassay (RIA). The mRNA expression of IL-1β and TNF-α was observed in synoviocytes using the reverse transcription (RT)-PCR method. The synoviocytes of the AA model were stimulated by Res or treated with the protein kinase C (PKC) inhibitor chelerythrine prior to stimulation. The expression of phosphorylated ERK1/2 (p-ERK1/2) was detected by western blotting. Res was able to reduce the elevated levels of IL-1β and TNF-α, and inhibit the mRNA expression of IL-1β and TNF-α in the synoviocytes of the AA model rats. VEGF expression in the Res-treated group was significantly lowered. The protein expression levels of p-ERK1/2 were significantly higher in the Res-treated group compared with those of the model group, while p-ERK1/2 was markedly lower in the group pretreated with chelerythrine. Res has a therapeutic effect on AA rats, which may be correlated with its immunoregulatory actions, and may activate p-ERK1/2 in synoviocytes via PKC.

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