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August 2013 Volume 6 Issue 2

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Article

Preventive effects of Lentinus edodes on homocysteinemia in mice

  • Authors:
    • Hyun Yang
    • Inho Hwang
    • Sun Kim
    • Changhwan Ahn
    • Eui-Ju Hong
    • Eui-Bae Jeung
  • View Affiliations / Copyright

    Affiliations: Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea
  • Pages: 465-468
    |
    Published online on: May 24, 2013
       https://doi.org/10.3892/etm.2013.1130
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Abstract

Homocysteinemia is associated with cardiovascular and neuronal degenerative diseases. Deficiencies of the B vitamins lead to high homocysteine serum levels. Lentinus edodes (L. edodes) is also known as the Shiitake mushroom and may have beneficial effects on vascular and lipid metabolic diseases, including hypertension, homocysteinemia and lipidemia. In this study, we induced a homocysteinemia-like condition in mice by the administration of a folate- and vitamin B12-deficient diet and evaluated the effect of L. edodes on the homocysteinemia-like condition. Homocysteinemia was induced by the administration of a diet deficient in folate and vitamin B12 (DFV) for 6 weeks to mice aged 4-10 weeks. The homocysteinemic mice were treated with L. edodes flour (5, 10 and 20%), eritadenine (10 mg/kg) or DFV only (negative control) for 2 weeks. The DFV induced a significant increase in serum homocysteine levels. The increased homocysteine serum levels were reduced by eritadenine and L. edodes flour (5, 10 and 20%). Hepatic levels of S-adenosyl-L-homocysteine hydrolase (SAH) were significantly higher under DFV administration and the elevated SAH levels were reduced by treatment with L. edodes in a dose‑dependent manner. The mRNA expression levels of DNA methyl transferases, DNMT1 and DNMT3a, were reduced in the DFV group, and the reduced levels of DNMT1 and DNMT3a mRNA expression were recovered in the eritadenine and L. edodes (5, 10 and 20%) groups. These results suggest that components of L. edodes, including eritadenine may have beneficial effects on hyperhomocysteinemia and its therapeutic effects may be involved in the regulation of DNA methylation-related genes in mice.
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Copy and paste a formatted citation
Spandidos Publications style
Yang H, Hwang I, Kim S, Ahn C, Hong E and Jeung E: Preventive effects of Lentinus edodes on homocysteinemia in mice. Exp Ther Med 6: 465-468, 2013.
APA
Yang, H., Hwang, I., Kim, S., Ahn, C., Hong, E., & Jeung, E. (2013). Preventive effects of Lentinus edodes on homocysteinemia in mice. Experimental and Therapeutic Medicine, 6, 465-468. https://doi.org/10.3892/etm.2013.1130
MLA
Yang, H., Hwang, I., Kim, S., Ahn, C., Hong, E., Jeung, E."Preventive effects of Lentinus edodes on homocysteinemia in mice". Experimental and Therapeutic Medicine 6.2 (2013): 465-468.
Chicago
Yang, H., Hwang, I., Kim, S., Ahn, C., Hong, E., Jeung, E."Preventive effects of Lentinus edodes on homocysteinemia in mice". Experimental and Therapeutic Medicine 6, no. 2 (2013): 465-468. https://doi.org/10.3892/etm.2013.1130
Copy and paste a formatted citation
x
Spandidos Publications style
Yang H, Hwang I, Kim S, Ahn C, Hong E and Jeung E: Preventive effects of Lentinus edodes on homocysteinemia in mice. Exp Ther Med 6: 465-468, 2013.
APA
Yang, H., Hwang, I., Kim, S., Ahn, C., Hong, E., & Jeung, E. (2013). Preventive effects of Lentinus edodes on homocysteinemia in mice. Experimental and Therapeutic Medicine, 6, 465-468. https://doi.org/10.3892/etm.2013.1130
MLA
Yang, H., Hwang, I., Kim, S., Ahn, C., Hong, E., Jeung, E."Preventive effects of Lentinus edodes on homocysteinemia in mice". Experimental and Therapeutic Medicine 6.2 (2013): 465-468.
Chicago
Yang, H., Hwang, I., Kim, S., Ahn, C., Hong, E., Jeung, E."Preventive effects of Lentinus edodes on homocysteinemia in mice". Experimental and Therapeutic Medicine 6, no. 2 (2013): 465-468. https://doi.org/10.3892/etm.2013.1130
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