Establishment and primary clinical application of competitive inhibition for measurement of augmenter of liver regeneration

Wang,Na; Sun,Hang; Tang,Lin; Deng,Jianchuan; Luo,Ya; Guo,Hui; Liu,Qi

doi:10.3892/etm.2013.1140

2014-January Volume 7 Issue 1

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2014-January Volume 7 Issue 1

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Article

Establishment and primary clinical application of competitive inhibition for measurement of augmenter of liver regeneration

Authors:
- Na Wang
- Hang Sun
- Lin Tang
- Jianchuan Deng
- Ya Luo
- Hui Guo
- Qi Liu
View Affiliations / Copyright

Affiliations: Institute for Viral Hepatitis and Department of Infectious Diseases, Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, P.R. China
Pages: 93-96
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Published online on: June 4, 2013

https://doi.org/10.3892/etm.2013.1140
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Abstract

The aim of the present study was to establish a quantitative method for the measurement of serum human augmenter of liver regeneration (hALR) using competitive inhibition that is applicable in the clinic. A monoclonal antibody to hALR was used as the primary antibody and the pure hALR protein was used as a standard for competition with Eu3+‑labeled hALR (Eu3+-hALR) to plot a standard curve. Serum samples from 90 patients with various liver diseases due to hepatitis B virus (HBV) infection were used for a competitive reaction with Eu3+-hALR. A regression analysis of the results was performed using the standard curve to calculate the serum concentration of hALR. The minimum detectable value using direct competitive measurement established by Eu3+-hALR was 1 ng/ml, with a positive linear correlation within the range of 200 ng/ml. In the sera of the 90 patients, the hALR level in the severe hepatitis group was the highest, followed by that in the acute hepatitis group. The serum hALR levels in the cirrhosis and chronic hepatitis groups were significantly higher compared with those in the normal control groups (P<0.01). The direct competitive measurement method of serum hALR established in the present study has high sensitivity, specificity, stability and reliability, meets clinical requirements and may be used as potential index in clinical tests.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Establishment and primary clinical application of competitive inhibition for measurement of augmenter of liver regeneration

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