Changes in monoclonal HLA‑DR antigen expression in acute organophosphorus pesticide‑poisoned patients

Xia,Chenyun; Wang,Mi; Liang,Qi; Yun,Ling'an; Kang,Housheng; Fan,Lei; Wang,Dongsheng; Zhang,Guoyuan

doi:10.3892/etm.2013.1356

Changes in monoclonal HLA‑DR antigen expression in acute organophosphorus pesticide‑poisoned patients

Authors:
- Chenyun Xia
- Mi Wang
- Qi Liang
- Ling'an Yun
- Housheng Kang
- Lei Fan
- Dongsheng Wang
- Guoyuan Zhang
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Affiliations: Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China, Department of Nephropathy, People's Hospital of Peking University, Beijing 100044, P.R. China
Published online on: October 22, 2013 https://doi.org/10.3892/etm.2013.1356
Pages: 137-140

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Abstract

The aim of this study was to investigate changes in human leukocyte antigen (HLA)‑DR expression of peripheral blood mononuclear cells (MNCs) in patients with acute organophosphorus pesticide poisoning (AOPP). HLA‑DR antigen expression of peripheral blood MNCs was examined in 75 patients with AOPP, including 36 patients without multiple organ dysfunction syndrome (non‑MODS) and 39 patients with multiple organ dysfunction syndrome (MODS), as well as in 30 healthy individuals using flow cytometry assay. The associations between HLA‑DR antigen expression and certain parameters were analyzed, including acute physiology and chronic health evaluation II (APACHE II) score, serum cholinesterase (ChE) activity, cardiac troponin I (cTnI), cardiac enzymes, and liver and kidney function. The mean fluorescence intensity (MCF) of HLA‑DR expression in the AOPP group (21.59±5.36) was significantly lower than that in the control group (27.85±4.86) (P<0.001). The MCF in the MODS group (18.17±4.23) was lower than that in the non‑MODS group (25.15±6.15). In addition, the MCF of the deceased patients (15.29±3.97) was lower than that of the surviving patients (22.34±2.76) (P<0.001). The MCF of patients with AOPP and MODS was positively correlated with serum ChE (P<0.01) and negatively correlated with the APACHE II score, creatine kinase isoenzyme, cTnI, lactate dehydrogenase, alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen and serum creatinine (P<0.05). In conclusion, HLA‑DR expression in patients with AOPP was significantly decreased compared with that in healthy individuals; HLA‑DR expression may therefore be a good indicator for evaluating AOPP, MODS disease severity, immune function, efficacy of prognosis and prognosis. Examination of HLA‑DR antigen expression may be of crucial clinical value.

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