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Article

Baicalin protects the myocardium from reperfusion‑induced damage in isolated rat hearts via the antioxidant and paracrine effect

  • Authors:
    • Feng Kong
    • Yun Luan
    • Zhao‑Hua Zhang
    • Guang‑Hui Cheng
    • Tong‑Gang Qi
    • Chao Sun
  • View Affiliations / Copyright

    Affiliations: Central Research Laboratory, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Department of Pediatrics, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China
  • Pages: 254-259
    |
    Published online on: October 29, 2013
       https://doi.org/10.3892/etm.2013.1369
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Abstract

The aim of the present study was to investigate the protective effect of baicalin (BA) against ischemia‑reperfusion (I/R) injury in isolated rat hearts. Sprague‑Dawley rat hearts were rapidly removed, mounted on a Langendorff apparatus and subjected to 30 min ischemia followed by 30 min reperfusion with Krebs‑Henseleit (K‑H) solution at 37˚C to establish the isolated I/R injury model. All animals (n=50) were randomly divided into five groups (n=10 in each): I, normal control; II, I/R; III, I/R plus 20 mg/kg BA; IV, I/R plus 40 mg/kg BA; and V, I/R plus 80 mg/kg BA. The degree of heart injury caused by the I/R was assessed by evaluating left ventricular function and by detecting the levels of lactate dehydrogenase (LDH) and creatine kinase (CK) in the coronary effluent and the myocardial superoxide dismutase (SOD) and malondialdehyde (MDA) levels in the isolated rat hearts. Myocardial infarct size and vascular density were assessed using histology and immunohistochemistry. The apoptotic cardiomyocytes were determined using flow cytometry (FCM). Compared with group II, the BA groups demonstrated improved left ventricular function, reduced CK and LDH release in the coronary effluent and increased SOD and MDA activity (P<0.05). Furthermore, histology and immunohistochemistry results showed that the infarct size was reduced and vessel density was augmented in the BA groups (P<0.01) compared with group II. The FCM results indicated that apoptosis was significantly lower in the BA groups than in group II (P<0.05) and that the protective effect was dose‑dependent. In conclusion, these results demonstrated that BA exerts a dose‑dependent protective effect on I/R injury in isolated rat hearts, the mechanisms of which may be associated with antioxidant and anti‑apoptosis properties. To the best of our knowledge, this study is the first evaluation of the efficacy of BA in isolated rat hearts using histology and immunohistochemistry, providing a foundation for the use of BA in the treatment of acute myocardial infarction.
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Copy and paste a formatted citation
Spandidos Publications style
Kong F, Luan Y, Zhang ZH, Cheng GH, Qi TG and Sun C: Baicalin protects the myocardium from reperfusion‑induced damage in isolated rat hearts via the antioxidant and paracrine effect. Exp Ther Med 7: 254-259, 2014.
APA
Kong, F., Luan, Y., Zhang, Z., Cheng, G., Qi, T., & Sun, C. (2014). Baicalin protects the myocardium from reperfusion‑induced damage in isolated rat hearts via the antioxidant and paracrine effect. Experimental and Therapeutic Medicine, 7, 254-259. https://doi.org/10.3892/etm.2013.1369
MLA
Kong, F., Luan, Y., Zhang, Z., Cheng, G., Qi, T., Sun, C."Baicalin protects the myocardium from reperfusion‑induced damage in isolated rat hearts via the antioxidant and paracrine effect". Experimental and Therapeutic Medicine 7.1 (2014): 254-259.
Chicago
Kong, F., Luan, Y., Zhang, Z., Cheng, G., Qi, T., Sun, C."Baicalin protects the myocardium from reperfusion‑induced damage in isolated rat hearts via the antioxidant and paracrine effect". Experimental and Therapeutic Medicine 7, no. 1 (2014): 254-259. https://doi.org/10.3892/etm.2013.1369
Copy and paste a formatted citation
x
Spandidos Publications style
Kong F, Luan Y, Zhang ZH, Cheng GH, Qi TG and Sun C: Baicalin protects the myocardium from reperfusion‑induced damage in isolated rat hearts via the antioxidant and paracrine effect. Exp Ther Med 7: 254-259, 2014.
APA
Kong, F., Luan, Y., Zhang, Z., Cheng, G., Qi, T., & Sun, C. (2014). Baicalin protects the myocardium from reperfusion‑induced damage in isolated rat hearts via the antioxidant and paracrine effect. Experimental and Therapeutic Medicine, 7, 254-259. https://doi.org/10.3892/etm.2013.1369
MLA
Kong, F., Luan, Y., Zhang, Z., Cheng, G., Qi, T., Sun, C."Baicalin protects the myocardium from reperfusion‑induced damage in isolated rat hearts via the antioxidant and paracrine effect". Experimental and Therapeutic Medicine 7.1 (2014): 254-259.
Chicago
Kong, F., Luan, Y., Zhang, Z., Cheng, G., Qi, T., Sun, C."Baicalin protects the myocardium from reperfusion‑induced damage in isolated rat hearts via the antioxidant and paracrine effect". Experimental and Therapeutic Medicine 7, no. 1 (2014): 254-259. https://doi.org/10.3892/etm.2013.1369
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