δEF1 upregulates CDK4 transcription via the E2‑box element on the CDK4 promoter

Hu,Fen; Wang,Kai; Zhang,Yunfeng; Gou,Lixia; Luo,Mengmeng; Zhang,Xiujun; Yang,Shuang

doi:10.3892/etm.2013.1376

2014-January Volume 7 Issue 1

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2014-January Volume 7 Issue 1

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Article

δEF1 upregulates CDK4 transcription via the E2‑box element on the CDK4 promoter

Authors:
- Fen Hu
- Kai Wang
- Yunfeng Zhang
- Lixia Gou
- Mengmeng Luo
- Xiujun Zhang
- Shuang Yang
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Affiliations: College of Life Sciences, Hebei United University, Tangshan, Hebei 063000, P.R. China, Department of Life Sciences, Tangshan Normal University, Tangshan, Hebei 063000, P.R. China, Medical College of Nankai University, Tianjin 300071, P.R. China
Pages: 161-164
|
Published online on: October 31, 2013

https://doi.org/10.3892/etm.2013.1376
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Abstract

The zinc finger‑homeodomain transcription factor, δ‑crystallin enhancer factor 1 (δEF1) has been identified as a regulatory factor involved in the promotion of breast cancer cell proliferation via the downregulation of p21 and the upregulation of cyclin‑dependent kinase‑2 (CDK2) and CDK4 expression. However, the molecular mechanisms underlying the regulation of CDK4 expression by δEF1 have not yet been elucidated. The present study demonstrated that the ectopic expression of δEF1 in MDA‑MB‑231 breast cancer cells significantly increased the activity of the CDK4 promoter. Deletion of the E2‑box (CACGTG), which is located at position ‑197/‑191 on the human CDK4 promoter, significantly attenuated the activation of CDK4 transcription by δEF1. In addition, a CDK4 promoter‑M construct was generated via site‑directed mutagenesis of the E2‑box on the human CDK4 promoter. Luciferase assay showed that the activation of CDK4 promoter‑M activity by δEF1 was markedly decreased compared with the CDK4‑promoter‑0.4k promoter. Knockdown of δEF1 using RNA interference resulted in the inhibition of CDK4 promoter activity. These observations suggest that δEF1 upregulates CDK4 transcription via the E2‑box element on the CDK4 promoter.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

δEF1 upregulates CDK4 transcription via the E2‑box element on the CDK4 promoter

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