Effect of limb ischemic preconditioning on myocardial apoptosis-related proteins in ischemia-reperfusion injury

Gao,Jianzhi; Zhao,Linjing; Wang,Yongling; Teng,Qinglei; Liang,Lidong; Zhang,Jinying

doi:10.3892/etm.2013.977

May 2013 Volume 5 Issue 5

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May 2013 Volume 5 Issue 5

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Article

Effect of limb ischemic preconditioning on myocardial apoptosis-related proteins in ischemia-reperfusion injury

Authors:
- Jianzhi Gao
- Linjing Zhao
- Yongling Wang
- Qinglei Teng
- Lidong Liang
- Jinying Zhang
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Affiliations: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Anesthesia, Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Cardiology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China
Pages: 1305-1309
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Published online on: February 26, 2013

https://doi.org/10.3892/etm.2013.977
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Abstract

The aim of this study was to investigate the effect of limb ischemic preconditioning (LIPC) on myocardial apoptosis in myocardial ischemia-reperfusion injury (MIRI), as well as the regulation of caspase-3 and the B cell lymphoma 2 (Bcl-2) gene in LIPC. A total of 50 rats were divided randomly into 5 groups (n=10). Four rats in each group were drawn out for detection of apoptosis. The sham, MIRI and LIPC groups underwent surgery without additional treatment. In the LY294002 group, LY294002 preconditioning was administered 15 min before reperfusion. In the LY294002+LIPC group, following LIPC, LY294002 was administered 15 min before reperfusion. The relative expression of myocardial Bcl-2 and caspase-3 mRNA and the apoptotic index for each group were determined by reverse transcription-polymerase chain reaction (RT-PCR) and terminal deoxynucleotidyl transferase deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL), respectively. The ultrastructure of the cardiac muscle tissues was observed by election microscopy. Compared with the sham group, the expression of caspase-3 mRNA in the MIRI group significantly increased (P<0.05) and the expression of Bcl-2 mRNA clearly decreased. Compared with the MIRI group, LIPC reduced the expression of caspase-3 and increased the expression of Bcl-2 mRNA (P<0.05). There were no significant differences between the LY294002+LIPC group and the MIRI group. Compared with the sham group, the apoptotic index of myocardial cells in the MIRI group significantly increased (P<0.05). Compared with the MIRI group, LIPC significantly decreased the apoptotic index of myocardial cells (P<0.05) and LY294002 increased the apoptotic index of myocardial cells. Compared with the LIPC group, LY294002+LIPC significantly increased the apoptotic index of myocardial cells (P<0.05). There were no significant differences between the LY294002+LIPC and MIRI groups. In conclusion, LIPC increased the expression of Bcl-2 and decreased caspase-3 mRNA and apoptosis in myocardial tissue following MIRI. Therefore, LIPC plays a protective role in myocardial tissue.

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1.	Malmberg M, Parkka J, Vahasilta T, et al: Cardiomyocyte apoptosis after cardioplegic ischemia: comparison to unprotected regional ischemia-reperfusion. Eur Surg Res. 46:19–25. 2011. View Article : Google Scholar : PubMed/NCBI
2.	Vilahur G, Juan-Babot O, Peña E, et al: Molecular and cellular mechanisms involved in cardiac remodeling after acute myocardial infarction. J Mol Cell Cardiol. 50:522–533. 2011. View Article : Google Scholar : PubMed/NCBI
3.	Campisi J: Cellular senescence and apoptosis: how cellular responses might influence aging phenotypes. Exp Gerontol. 38:5–11. 2003. View Article : Google Scholar : PubMed/NCBI
4.	Chen Z, Chua CC, Gao J, et al: Prevention of ischemia/reperfusion induced cardiac apoptosis and injury by melatonin is independent of glutathione peroxdiase 1. J Pineal Res. 46:235–241. 2009. View Article : Google Scholar : PubMed/NCBI
5.	Wang J and Li J: Activated protein C: a potential cardioprotective factor against ischemic injury during ischemia/reperfusion. Am J Transl Res. 151:381–392. 2009.PubMed/NCBI
6.	Wu L, Qiao H, Li Y, et al: Protective roles of puerarin and Danshensu on acute ischemic myocardial injury in rats. Phytomedicine. 14:652–658. 2007. View Article : Google Scholar : PubMed/NCBI
7.	Kawaguchi M, Takahashi M, Hata T, et al: Inflammasome activation of cardiac fibroblasts is essential for myocardial ischemia/reperfusion injury. Circulation. 23:594–604. 2011. View Article : Google Scholar
8.	Wang Y, Lau WB, Gao E, et al: Cardiomyocyte-derived adiponectin is biologically active in protecting against myocardial ischemia-reperfusion injury. Am J Physiol Endocrinol Metab. 298:E663–E670. 2010. View Article : Google Scholar : PubMed/NCBI
9.	Zhao L, Wang Y, Min X, et al: Ischemia-reperfusion injury up-regulates Pim-3 gene expression in myocardial tissue. J Huazhong Univ Sci Technolog Med Sci. 30:704–708. 2010. View Article : Google Scholar : PubMed/NCBI
10.	Maiuri MC, Criolo A, Tasdemir E, et al: BH3-only proteins and BH3 mimetics induce autophagy by competitively disrupting the interaction between Beclin 1 and Bcl-2/Bcl-X(L). Autophagy. 3:374–376. 2007. View Article : Google Scholar
11.	Baldi A, Abbate A, Bussani R, et al: Apoptosis and post-infarction left ventricular remodeling. J Mol Cell Cardiol. 34:165–174. 2002. View Article : Google Scholar : PubMed/NCBI
12.	Suo GJ, Qin J, Zhong CP, et al: Suppressor of cytokine signaling 1 inhibits apoptosis of islet grafts through caspase 3 and apoptosis-inducing factor pathways in rats. Transplant Proc. 42:2658–2661. 2010. View Article : Google Scholar : PubMed/NCBI
13.	Penna C, Perrelli MG, Raimondo S, et al: Postconditioning induces an antiapoptotic effect and preserves mitochondrial integrity in isolated rat hearts. Biochim Biophys Acta. 1787:794–801. 2009. View Article : Google Scholar : PubMed/NCBI
14.	Jin YC, Kim KJ, Kim YM, et al: Anti-apoptotic effect of magnolol in myocardial ischemia and reperfusion injury requires extracellular signal-regulated kinase1/2 pathways in rat in vivo. Exp Biol Med (Maywood). 233:1280–1288. 2008. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of limb ischemic preconditioning on myocardial apoptosis-related proteins in ischemia-reperfusion injury

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