Hyperglycemia induces endoplasmic reticulum stress‑dependent CHOP expression in osteoblasts

  • Authors:
    • Wei Liu
    • Xiaoqing Zhu
    • Qian Wang
    • Linlin Wang
  • View Affiliations

  • Published online on: February 27, 2013     https://doi.org/10.3892/etm.2013.978
  • Pages: 1289-1292
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Diabetic osteoporosis is a metabolic bone disease responsible for global health problems. Hyperglycemia induces osteopenia, increases bone fragility and unbalances the coupling of osteoblasts and osteoclasts. The mechanism is, however, unknown. For the purpose of this study, we hypothesized that hyperglycemia destroys endoplasmic reticulum (ER) homeostasis, activates C/EBP-homologous protein (CHOP) and induces osteoblast apoptosis under diabetic conditions. Diabetic rats were created by injecting streptozotocin (STZ) 65 mg/kg intraperitoneally and their osteoblasts were cultured under high-glucose medium in vitro. The bone mineral density (BMD) and pathological changes of the rats' femurs were observed. The expression of CHOP in osteoblasts was assayed using immunohistochemistry and western blot analysis. Six weeks after diabetic model establishment, a significant decrease was found in the BMD of the diabetic rat femurs, and the numbers of osteoblasts under cortical bone were also reduced. The expression of the ER stress regulator CHOP in osteoblasts of diabetic rats or high-glucose medium was also elevated (P<0.01). The present results demonstrated that hyperglycemia elevated the expression of CHOP and finally led to osteoporosis. This suggested that elevating the expression of CHOP may play a role in diabetic osteoporosis.
View Figures
View References

Related Articles

Journal Cover

May 2013
Volume 5 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Liu W, Zhu X, Wang Q and Wang L: Hyperglycemia induces endoplasmic reticulum stress‑dependent CHOP expression in osteoblasts. Exp Ther Med 5: 1289-1292, 2013
APA
Liu, W., Zhu, X., Wang, Q., & Wang, L. (2013). Hyperglycemia induces endoplasmic reticulum stress‑dependent CHOP expression in osteoblasts. Experimental and Therapeutic Medicine, 5, 1289-1292. https://doi.org/10.3892/etm.2013.978
MLA
Liu, W., Zhu, X., Wang, Q., Wang, L."Hyperglycemia induces endoplasmic reticulum stress‑dependent CHOP expression in osteoblasts". Experimental and Therapeutic Medicine 5.5 (2013): 1289-1292.
Chicago
Liu, W., Zhu, X., Wang, Q., Wang, L."Hyperglycemia induces endoplasmic reticulum stress‑dependent CHOP expression in osteoblasts". Experimental and Therapeutic Medicine 5, no. 5 (2013): 1289-1292. https://doi.org/10.3892/etm.2013.978