Establishment and performance assessment of preparation technology of internal quality control products for blood transfusion compatibility testing

Yu,Yang; Ma,Chunya; Feng,Qian; Chen,Xin; Guan,Xiaozhen; Zhang,Xiaojuan; Chen,Linfeng; Lin,Zilin; Pan,Jichun; Zhang,Ting; Luo,Qun; Wang,Deqing

doi:10.3892/etm.2013.994

May 2013 Volume 5 Issue 5

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May 2013 Volume 5 Issue 5

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Article

Establishment and performance assessment of preparation technology of internal quality control products for blood transfusion compatibility testing

Authors:
- Yang Yu
- Chunya Ma
- Qian Feng
- Xin Chen
- Xiaozhen Guan
- Xiaojuan Zhang
- Linfeng Chen
- Zilin Lin
- Jichun Pan
- Ting Zhang
- Qun Luo
- Deqing Wang
View Affiliations / Copyright

Affiliations: Department of Blood Transfusion of PLA General Hospital, Clinical Blood Transfusion Centre of PLA, Beijing 100853, P.R. China
Pages: 1466-1470
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Published online on: March 7, 2013

https://doi.org/10.3892/etm.2013.994
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Abstract

The aim of this study was to establish and to optimize the preparation technology of whole blood internal quality control (IQC) products for blood transfusion compatibility testing. Several B-type RhD-negative blood samples collected from healthy donors were mixed. Two groups of whole blood IQC products, namely, the preservative solution group (PS group) and the saline group, were prepared. The agglutination intensity of IQC sample red cells and anti-B antibody, IgM anti-A antibody and reverse-typing A cell, IgG anti-D and O-type RhD-positive red cells, as well as free hemoglobin concentration in the supernatant of the two groups were detected. The erythrocytes in both groups were damaged to a certain extent during storage, but no evident (above moderate) hemolysis was observed in the stored sample within 42 days. The red cells remained structurally complete and the reaction activity of IgG anti-D reagent remained generally unchanged (P>0.05). Although the reaction activity oscillation of IgM anti-A reagent was observed, the agglutination intensity varied within an acceptable range of 1+. No difference was observed between the preparation methods of the samples, i.e., between the erythrocyte washed with saline and the one washed with red cell preservative solution (P>0.05). The long shelf life, low variance between tubes and stable antigen-antibody reaction activity of the whole blood IQC products prepared using the proposed method can meet the requirements of blood transfusion compatibility testing.

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1.	Levey S and Jennings ER: The use of control charts in the clinical laboratory. Am J Clin Pathol. 20:1059–1066. 1950.PubMed/NCBI
2.	Lewis SM: Quality assurance programmes in the United Kingdom. Ann Ist Super Sanita. 31:53–59. 1995.
3.	World Health Organization: Strategies for Safe Blood Transfusion. World Health Organization; Geneva: pp. 511998
4.	Vidal JL, Vega AB, López FJ and Frenich AG: Application of internal quality control to the analysis of quaternary ammonium compounds in surface and groundwater from Andalusia (Spain) by liquid chromatography with mass spectrometry. J Chromatogr A. 1050:179–184. 2004. View Article : Google Scholar
5.	Lock RJ: My approach to internal quality control in a clinical immunology laboratory. J Clin Pathol. 59:681–684. 2006. View Article : Google Scholar : PubMed/NCBI
6.	Chapman JF, Elliott C, Knowles SM, Milkins CE and Poole GD: Guidelines for compatibility procedures in blood transfusion laboratories. Transfus Med. 14:59–73. 2004. View Article : Google Scholar : PubMed/NCBI
7.	American Association of Blood Banks: Technical Manual. 16th edition. Maryland, USA: pp. 15–39. 2008
8.	Scientific Subcommittee of the Australian and New Zealand Society of Blood Transfusion Inc: Guidelines for Pretransfusion Testing. 4th edition. Sydney, Australia: pp. 19–23. 2002
9.	Chinese Ministry of Health: Accreditation standards of level 3 comprehensive hospital (2011 version). Beijing, China: pp. 1252012
10.	Yu Y, Ma CY, Feng Q, et al: Inside quality control for whole blood preservation performed at blood transfusion compatibility testing laboratory. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 18:780–784. 2010.(In Chinese).
11.	National Blood Service: Guidelines for the Blood Transfusion Services in the United Kingdom (7th edition). 148–175. 2005.
12.	International Organization for Standardization (ISO): Medical laboratories - particular requirements for quality and competence. Geneva, Switzerland: ISO Standard 15189. 5.6. 2007
13.	Thompson M and Wood R: Harmonised guidelines for IQC in analytical chemistry laboratories. Pure Appl Chem. 67:649–666. 1995. View Article : Google Scholar
14.	Petersen PH, Ricós C, Stöckl D, et al: Proposed guidelines for the internal quality control of analytical results in the medical laboratory. Eur J Clin Chem Clin Biochem. 34:983–999. 1996.PubMed/NCBI
15.	Thompson M: Internal quality control in routine analysis. Royal Soc Chem. 2010.
16.	Gardner MJ: The quotation overleaf is from quality control techniques for chemical analysis: some current shortcomings and possible future developments. Accred Qual Assur. 12:653–657. 2007. View Article : Google Scholar
17.	D’Alessandro A, Liumbruno G, Grazzini G and Zolla L: Red blood cell storage: the story so far. Blood Transfus. 8:82–88. 2010.PubMed/NCBI
18.	Mollison PL: The introduction of citrate as an anticoagulant and of glucose as a red cell preservative. Br J Haematol. 108:13–18. 2000. View Article : Google Scholar : PubMed/NCBI
19.	Hess JR and Greenwalt TJ: Storage of red blood cells: new approaches. Transfus Med Rev. 16:283–295. 2002. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Establishment and performance assessment of preparation technology of internal quality control products for blood transfusion compatibility testing

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