Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
2014-April Volume 7 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
2014-April Volume 7 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Connexin expression patterns in diseased human corneas

  • Authors:
    • Jiajie Zhai
    • Qin Wang
    • Liang Tao
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong 510040, P.R. China
  • Pages: 791-798
    |
    Published online on: February 10, 2014
       https://doi.org/10.3892/etm.2014.1530
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to explore the feasibility of using antisense connexin (Cx) treatment to promote corneal wound healing, and to investigate the changes of Cx gap junction proteins in terms of mRNA, protein expression and distribution in human corneas that were diseased due to various causes. A total of 13 diseased corneas were studied, which were obtained from five eyes injured by chemical burns, five infected eyes and three eyes with Stevens‑Johnson syndrome (SJS)‑affected corneas. Total RNA was extracted from the corneas and processed by qPCR with isoform primers to detect the expression of eight Cxs. Flow cytometry was adopted to determine the differences in the expression levels of Cx26, Cx31.1 and Cx43. Immunofluorescence was employed to show the localization of the three aforementioned Cxs. The qPCR results indicated that of the eight Cxs, only Cx26, Cx31.1 and Cx43 were upregulated in diseased corneas. Flow cytometry showed that all the diseased corneal tissues, with the exception of the SJS-affected corneas, showed a significantly higher percentage of cells that expressed Cx26 and Cx31.1 compared with the percentage in normal corneas (P<0.05). For Cx43, all three injured corneal groups showed a significantly higher percentage of cells that expressed Cx43 compared with the percentage in normal corneas (P<0.05). Immunohistochemical staining showed that the localization of Cx26, Cx31.1 and Cx43 differed between normal corneas and diseased corneas. This study elucidated the alteration of Cx expression patterns in several corneal diseases. The results indicated that Cx26, Cx31.1 and Cx43 are upregulated in chemically burned and infected corneas at the mRNA and protein levels, whereas only Cx43 is upregulated in SJS-affected corneas.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Cao F, Eckert R, Elfgang C, et al: A quantitative analysis of connexin-specific permeability differences of gap junctions expressed in HeLa transfectants and Xenopus oocytes. J Cell Sci. 111:31–43. 1998.PubMed/NCBI

2 

Kumar NM and Gilula NB: The gap junction communication channel. Cell. 84:381–388. 1996. View Article : Google Scholar : PubMed/NCBI

3 

Yeager M and Nicholson BJ: Structure of gap junction intercellular channels. Curr Opin Struct Biol. 6:183–192. 1996. View Article : Google Scholar

4 

Chin KY: Connexins, a new target in wound treatment. J Wound Care. 20:386–390. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Ormonde S, Chou CY, Goold L, et al: Regulation of connexin43 gap junction protein triggers vascular recovery and healing in human ocular persistent epithelial defect wounds. J Membr Biol. 245:381–388. 2012. View Article : Google Scholar

6 

Chang CY, Laux-Fenton WT, Law LY, et al: Antisense down regulation of connexin31.1 reduces apoptosis and increases thickness of human and animal corneal epithelia. Cell Biol Int. 33:376–385. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Hughes WF Jr: Alkali burns of the eye; review of the literature and summary of present knowledge. Arch Ophthal. 35:423–449. 1946. View Article : Google Scholar : PubMed/NCBI

8 

WF H: Alkali burns of the eye; clinical and pathologic course. Arch Ophthal. 36:189–214. 1946. View Article : Google Scholar

9 

Wagoner MD: Chemical injuries of the eye: current concepts in pathophysiology and therapy. Surv Ophthalmol. 41:275–313. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Lin A, Patel N, Yoo D, DeMartelaere S and Bouchard C: Management of ocular conditions in the burn unit: thermal and chemical burns and Stevens-Johnson syndrome/toxic epidermal necrolysis. J Burn Care Res. 32:547–560. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Chang YS, Huang FC, Tseng SH, et al: Erythema multiforme, Stevens-Johnson syndrome, and toxic epidermal necrolysis: acute ocular manifestations, causes, and management. Cornea. 26:123–129. 2007. View Article : Google Scholar

12 

Yip LW, Thong BY, Lim J, Tan AW, Wong HB, Handa S and Heng WJ: Ocular manifestations and complications of Stevens-Johnson syndrome and toxic epidermal necrolysis: an Asian series. Allergy. 62:527–531. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Hynes AY, Kafkala C, Daoud YJ and Foster CS: Controversy in the use of high-dose systemic steroids in the acute care of patients with Stevens-Johnson syndrome. Int Ophthalmol Clin. 45:25–48. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Oliva MS, Schottman T and Gulati M: Turning the tide of corneal blindness. Indian J Ophthalmol. 60:423–427. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Kawakita T, Higa K, Shimmura S, et al: Fate of corneal epithelial cells separated from limbus in vivo. Invest Ophthalmol Vis Sci. 52:8132–8137. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Chan KY, Patton DL and Cosgrove YT: Time-lapse videomicroscopic study of in vitro wound closure in rabbit corneal cells. Invest Ophthalmol Vis Sci. 30:2488–2498. 1989.PubMed/NCBI

17 

Matic M, Petrov IN, Rosenfeld T and Wolosin JM: Alterations in connexin expression and cell communication in healing corneal epithelium. Invest Ophthalmol Vis Sci. 38:600–609. 1997.PubMed/NCBI

18 

Schlingmann B, Schadzek P, Busko S, Heisterkamp A and Ngezahayo A: Cataract-associated D3Y mutation of human connexin46 (hCx46) increases the dye coupling of gap junction channels and suppresses the voltage sensitivity of hemichannels. J Bioenerg Biomembr. 44:607–614. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Scherer SS and Kleopa KA: X-linked Charcot-Marie-Tooth disease. J Peripher Nerv Syst. 17:9–13. 2012. View Article : Google Scholar

20 

Wangemann P: Supporting sensory transduction: cochlear fluid homeostasis and the endocochlear potential. J Physiol. 576:11–21. 2006. View Article : Google Scholar : PubMed/NCBI

21 

de Zwart-Storm EA, Rosa RF, Martin PE, et al: Molecular analysis of connexin26 asparagine14 mutations associated with syndromic skin phenotypes. Exp Dermatol. 20:408–412. 2011.PubMed/NCBI

22 

Lorentz R, Shao Q, Huang T, Fishman GI and Laird DW: Characterization of gap junction proteins in the bladder of Cx43 mutant mouse models of oculodentodigital dysplasia. J Membr Biol. 245:345–355. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Gomes P, Srinivas SP, Van Driessche W, Vereecke J and Himpens B: ATP release through connexin hemichannels in corneal endothelial cells. Invest Ophthalmol Vis Sci. 46:1208–1218. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Yuan X, Chen Z, Yang Z, et al: Expression pattern of connexins in the corneal and limbal epithelium of a primate. Cornea. 28:194–199. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Laux-Fenton WT, Donaldson PJ, Kistler J and Green CR: Connexin expression patterns in the rat cornea: molecular evidence for communication compartments. Cornea. 22:457–464. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Dong Y, Roos M, Gruijters T, et al: Differential expression of two gap junction proteins in corneal epithelium. Eur J Cell Biol. 64:95–100. 1994.PubMed/NCBI

27 

Spanakis SG, Petridou S and Masur SK: Functional gap junctions in corneal fibroblasts and myofibroblasts. Invest Ophthalmol Vis Sci. 39:1320–1328. 1998.PubMed/NCBI

28 

Matic M, Petrov IN, Chen S, et al: Stem cells of the corneal epithelium lacks connexins and metabolite transfer capacity. Differentiation. 61:251–260. 1997. View Article : Google Scholar : PubMed/NCBI

29 

Wolosin JM, Budak MT and Akinci MA: Ocular surface epithelial and stem cell development. Int J Dev Biol. 48:981–991. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Ratkay-Traub I, Hopp B, Bor Z, et al: Regeneration of rabbit cornea following excimer laser photorefractive keratectomy: a study on gap junctions, epithelial junctions and epidermal growth factor receptor expression in correlation with cell proliferation. Exp Eye Res. 73:291–302. 2001. View Article : Google Scholar

31 

Laux-Fenton W: The role of connexins in corneal homeostasis and repair: mastering the connections to improve repair (PhD thesis). University of Auckland; Auckland, New Zealand: 2003

32 

Grupcheva CN, Laux WT, Rupenthal ID, McGhee J, McGhee CN and Green CR: Improved corneal wound healing through modulation of gap junction communication using connexin43-specific antisense oligodeoxynucleotides. Invest Ophthalmol Vis Sci. 53:1130–1138. 2012. View Article : Google Scholar

33 

Oviedo-Orta E and Howard Evans W: Gap junctions and connexin-mediated communication in the immune system. Biochim Biophys Acta. 1662:102–112. 2004. View Article : Google Scholar : PubMed/NCBI

34 

Vera LS, Gueudry J, Delcampe A, et al: In vivo confocal microscopic evaluation of corneal changes in chronic Stevens-Johnson syndrome and toxic epidermal necrolysis. Cornea. 28:401–407. 2009. View Article : Google Scholar : PubMed/NCBI

35 

No authors listed. Gap junction-mediated intercellular signalling in health and disease. In: Proceedings of a meeting; London, United Kingdom. 2–5 March 1998; Novartis Found Symp. 219. pp. 1–284. 1999

36 

Harris AL: Emerging issues of connexin channels: biophysics fills the gap. Q Rev Biophys. 34:325–472. 2001. View Article : Google Scholar : PubMed/NCBI

37 

Laux-Fenton WT, Donaldson PJ, Kistler J and Green CR: Connexin expression patterns in the rat cornea: molecular evidence for communication compartments. Cornea. 22:457–464. 2003. View Article : Google Scholar : PubMed/NCBI

38 

Coutinho P, Qiu C, Frank S, Tamber K and Becker D: Dynamic changes in connexin expression correlate with key events in the wound healing process. Cell Biol Int. 27:525–541. 2003. View Article : Google Scholar : PubMed/NCBI

39 

Goliger JA and Paul DL: Wounding alters epidermal connexin expression and gap junction-mediated intercellular communication. Mol Biol Cell. 6:1491–1501. 1995. View Article : Google Scholar : PubMed/NCBI

40 

Wilson SE: Corneal myofibroblast biology and pathobiology: generation, persistence, and transparency. Exp Eye Res. 99:78–88. 2012. View Article : Google Scholar : PubMed/NCBI

41 

Coutinho P, Qiu C, Frank S, et al: Limiting burn extension by transient inhibition of Connexin43 expression at the site of injury. Br J Plast Surg. 58:658–667. 2005. View Article : Google Scholar : PubMed/NCBI

42 

Djalilian AR, McGaughey D, Patel S, Seo EY, Yang C, Cheng J, Tomic M, Sinha S, Ishida-Yamamoto A and Segre JA: Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response. J Clin Invest. 116:1243–1253. 2006. View Article : Google Scholar : PubMed/NCBI

43 

de Zwart-Storm EA, Rosa RF, Martin PE, Foelster-Holst R, Frank J, Bau AE, Zen PR, Graziadio C, Paskulin GA, Kamps MA, van Geel M and van Steensel MA: Molecular analysis of connexin26 asparagine14 mutations associated with syndromic skin phenotypes. Exp Dermatol. 20:408–412. 2011.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhai J, Wang Q and Tao L: Connexin expression patterns in diseased human corneas. Exp Ther Med 7: 791-798, 2014.
APA
Zhai, J., Wang, Q., & Tao, L. (2014). Connexin expression patterns in diseased human corneas. Experimental and Therapeutic Medicine, 7, 791-798. https://doi.org/10.3892/etm.2014.1530
MLA
Zhai, J., Wang, Q., Tao, L."Connexin expression patterns in diseased human corneas". Experimental and Therapeutic Medicine 7.4 (2014): 791-798.
Chicago
Zhai, J., Wang, Q., Tao, L."Connexin expression patterns in diseased human corneas". Experimental and Therapeutic Medicine 7, no. 4 (2014): 791-798. https://doi.org/10.3892/etm.2014.1530
Copy and paste a formatted citation
x
Spandidos Publications style
Zhai J, Wang Q and Tao L: Connexin expression patterns in diseased human corneas. Exp Ther Med 7: 791-798, 2014.
APA
Zhai, J., Wang, Q., & Tao, L. (2014). Connexin expression patterns in diseased human corneas. Experimental and Therapeutic Medicine, 7, 791-798. https://doi.org/10.3892/etm.2014.1530
MLA
Zhai, J., Wang, Q., Tao, L."Connexin expression patterns in diseased human corneas". Experimental and Therapeutic Medicine 7.4 (2014): 791-798.
Chicago
Zhai, J., Wang, Q., Tao, L."Connexin expression patterns in diseased human corneas". Experimental and Therapeutic Medicine 7, no. 4 (2014): 791-798. https://doi.org/10.3892/etm.2014.1530
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team