Antagonistic effect of protein extracts from Streptococcus sanguinis on pathogenic bacteria and fungi of the oral cavity

Ma,Shengli; Li,Hui; Yan,Chuang; Wang,Dan; Li,Haiqing; Xia,Xue; Dong,Xue; Zhao,Yingnan; Sun,Tingting; Hu,Pengfei; Guan,Weijun

doi:10.3892/etm.2014.1618

June-2014 Volume 7 Issue 6

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June-2014 Volume 7 Issue 6

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Antagonistic effect of protein extracts from Streptococcus sanguinis on pathogenic bacteria and fungi of the oral cavity

Authors:
- Shengli Ma
- Hui Li
- Chuang Yan
- Dan Wang
- Haiqing Li
- Xue Xia
- Xue Dong
- Yingnan Zhao
- Tingting Sun
- Pengfei Hu
- Weijun Guan
View Affiliations / Copyright

Affiliations: Department of Stomatology, Hospital of Heilongjiang Province, Harbin, Heilongjiang 150036, P.R. China, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China
Pages: 1486-1494
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Published online on: March 12, 2014

https://doi.org/10.3892/etm.2014.1618
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Abstract

An antibacterial substance from Streptococcus sanguinis (S. sanguinis) is known to have an inhibitory effect on putative periodontal pathogens, but its inhibitory effect on pathogens of oral candidiasis is unknown. In this study, intracellular and exocrine proteins were extracted from S. sanguinis. The antagonistic effect of the protein extracts on Prevotella intermedia (P. intermedia) and Porphyromonas gingivalis (P. gingivalis) was detected by a well‑plate technique, and the effects of the protein extracts on biofilms formed by these bacteria were evaluated by confocal laser scanning microscopy. The antagonistic effect of the protein extracts on pathogenic fungi was investigated using Candida albicans (C. albicans) and Candida tropicalis (C. tropicalis). The growth curves of C. albicans and C. tropicalis were determined from ultraviolet absorption measurements, their morphological changes following treatment were observed by optical microscopy and scanning electron microscopy, and the effects of the protein extracts on the thickness of their biofilms and the distribution of dead/live bacteria within the biofilms were detected by confocal laser scanning microscopy. The results showed significant inhibitory effects of the intracellular proteins extracted from S. sanguinis on pathogenic bacteria (P. intermedia and P. gingivalis), fungi (C. albicans and C. tropicalis) and the biofilms formed by them. Furthermore, the growth curves and morphology of C. albicans and C. tropicalis were altered following treatment with the intracellular proteins, resulting in disc‑like depressions in the surfaces of the fungal spores and mycelia. By contrast, the exocrine proteins demonstrated no significant inhibitory effect on the pathogenic bacteria, fungi and the biofilms formed by them. Thus, it may be concluded that intracellular proteins of S. sanguinis have antibacterial activity and exert an antagonistic effect on certain pathogenic bacteria and fungi of the oral cavity.

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International Journal of Molecular Medicine

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Oncology Letters

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Molecular and Clinical Oncology

World Academy of Sciences Journal

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International Journal of Epigenetics

Medicine International

Antagonistic effect of protein extracts from Streptococcus sanguinis on pathogenic bacteria and fungi of the oral cavity

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