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Resolvin E1 reduces hepatic fibrosis in mice with Schistosoma japonicum infection

  • Authors:
    • Wenhong Qiu
    • Kaiwen Guo
    • Luyang Yi
    • Yeli Gong
    • Lixia Huang
    • Wei Zhong
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, School of Medicine, Jianghan University, Wuhan, Hubei 430056, P.R. China, Department of Immunology, Wuhan University of Science and Technology, Wuhan, Hubei 430080, P.R. China
  • Pages: 1481-1485
    |
    Published online on: March 27, 2014
       https://doi.org/10.3892/etm.2014.1641
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Abstract

The aim of this study was to investigate whether resolvin E1 (RvE1) protects against hepatic fibrosis in a murine model of liver fibrosis induced by Schistosoma japonicum infection. A total of 30 pathogen‑free Kunming mice were randomly and equally divided into three groups: Control (uninfected, untreated), model (infected, untreated) and RvE1 intervention (infected, RvE1‑treated; 100 ng daily). The mice were infected with Schistosoma japonicum by inoculating the abdominal skin with 20±2 cercariae to induce models of liver fibrosis. The area and numbers of the granulomas in the livers were assessed through histopathology after 70 days of treatment. The levels of tumor necrosis factor (TNF)‑α and interferon (IFN)‑γ were evaluated in the serum by enzyme‑linked immu­nosorbent assay (ELISA). The expression levels of TNF‑α were detected in the hepatic tissue by reverse transcription‑polymerase chain reaction and western blot analysis. The activity levels of alanine aminotransferase and aspartate aminotransferase were determined in the serum by ELISA. The expression levels of laminin (LN), hyaluronic acid (HA), procollagen type III (PC‑III) and type IV collagen (IV‑C) were detected in the serum by radioimmunoassays. The results revealed that the mean area of the granulomas was smaller in the RvE1 intervention group compared with that in the model group. Following RvE1 treatment, the serum levels of TNF‑α were lower than those in the model group, while the serum levels of IFN‑γ were higher compared with those in the model group. The expression levels of TNF‑α were lower in the hepatic tissue following RvE1 treatment compared with those in the model group. The indicators of liver fibrosis, the levels of LN, HA, PC‑III and IV‑C in the serum, were lower following RvE1 treatment than those in the model group. In conclusion, RvE1 treatment may reduce the growth of granulomas, thereby slowing the process of hepatic fibrosis, and this effect may be the result of anti‑inflammatory and immune system adjustment.
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Copy and paste a formatted citation
Spandidos Publications style
Qiu W, Guo K, Yi L, Gong Y, Huang L and Zhong W: Resolvin E1 reduces hepatic fibrosis in mice with Schistosoma japonicum infection. Exp Ther Med 7: 1481-1485, 2014.
APA
Qiu, W., Guo, K., Yi, L., Gong, Y., Huang, L., & Zhong, W. (2014). Resolvin E1 reduces hepatic fibrosis in mice with Schistosoma japonicum infection. Experimental and Therapeutic Medicine, 7, 1481-1485. https://doi.org/10.3892/etm.2014.1641
MLA
Qiu, W., Guo, K., Yi, L., Gong, Y., Huang, L., Zhong, W."Resolvin E1 reduces hepatic fibrosis in mice with Schistosoma japonicum infection". Experimental and Therapeutic Medicine 7.6 (2014): 1481-1485.
Chicago
Qiu, W., Guo, K., Yi, L., Gong, Y., Huang, L., Zhong, W."Resolvin E1 reduces hepatic fibrosis in mice with Schistosoma japonicum infection". Experimental and Therapeutic Medicine 7, no. 6 (2014): 1481-1485. https://doi.org/10.3892/etm.2014.1641
Copy and paste a formatted citation
x
Spandidos Publications style
Qiu W, Guo K, Yi L, Gong Y, Huang L and Zhong W: Resolvin E1 reduces hepatic fibrosis in mice with Schistosoma japonicum infection. Exp Ther Med 7: 1481-1485, 2014.
APA
Qiu, W., Guo, K., Yi, L., Gong, Y., Huang, L., & Zhong, W. (2014). Resolvin E1 reduces hepatic fibrosis in mice with Schistosoma japonicum infection. Experimental and Therapeutic Medicine, 7, 1481-1485. https://doi.org/10.3892/etm.2014.1641
MLA
Qiu, W., Guo, K., Yi, L., Gong, Y., Huang, L., Zhong, W."Resolvin E1 reduces hepatic fibrosis in mice with Schistosoma japonicum infection". Experimental and Therapeutic Medicine 7.6 (2014): 1481-1485.
Chicago
Qiu, W., Guo, K., Yi, L., Gong, Y., Huang, L., Zhong, W."Resolvin E1 reduces hepatic fibrosis in mice with Schistosoma japonicum infection". Experimental and Therapeutic Medicine 7, no. 6 (2014): 1481-1485. https://doi.org/10.3892/etm.2014.1641
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