Resveratrol affects the expression of glutamate cysteine ligase in the kidneys of aged rats

Yuan,Jiangshui; Zhang,Zongliang; Li,Li; Song,Weiqing

doi:10.3892/etm.2014.1664

Resveratrol affects the expression of glutamate cysteine ligase in the kidneys of aged rats

Authors:
- Jiangshui Yuan
- Zongliang Zhang
- Li Li
- Weiqing Song
View Affiliations

Affiliations: Clinical Laboratory, Qingdao Municipal Hospital, Qingdao, Shandong 266011, P.R. China, Department of Urology, Qingdao Municipal Hospital, Qingdao, Shandong 266011, P.R. China
Published online on: April 3, 2014 https://doi.org/10.3892/etm.2014.1664
Pages: 1762-1766

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of the present study was to evaluate the effect that a dietary intake of resveratrol (RSV) had on the expression of glutamate cysteine ligase (GCL) in the kidneys of aged rats. Young, middle‑aged and aged rats were each randomly divided into two groups. The control groups were fed a controlled diet and the experimental groups received a controlled diet supplemented with RSV. GCL activity levels in the kidneys were determined. Protein content and relative gene expression levels of the two subunits of GCL were evaluated by western blot analysis and quantitative polymerase chain reaction, respectively. GCL activity levels significantly increased in the kidneys of aged rats fed the RSV‑supplemented diet. In addition, RSV markedly increased the protein content and relative mRNA expression levels of the GCL subunits in the kidneys of aged rats. These observations have important implications for the development of therapeutic agents for the kidneys that may enable the elderly population to combat oxidative stress.

View Figures

View References

1	Brown-Borg HM and Rakoczy SG: Glutathione metabolism in long-living Ames dwarf mice. Exp Gerontol. 40:115–120. 2005. View Article : Google Scholar : PubMed/NCBI
2	Chen CN, Brown-Borg HM, Rakoczy SG, Ferrington DA and Thompson LV: Aging impairs the expression of the catalytic subunit of glutamate cysteine ligase in soleus muscle under stress. J Gerontol A Biol Sci Med Sci. 65:129–137. 2010. View Article : Google Scholar : PubMed/NCBI
3	Maher P: The effects of stress and aging on glutathione metabolism. Ageing Res Rev. 4:288–314. 2005. View Article : Google Scholar : PubMed/NCBI
4	Leeuwenburgh C, Fiebig R, Chandwaney R and Ji LL: Aging and exercise training in skeletal muscle: responses of glutathione and antioxidant enzyme systems. Am J Physiol. 267:R439–R445. 1994.PubMed/NCBI
5	Chen CN, Brown-Borg HM, Rakoczy SG and Thompson LV: Muscle disuse: adaptation of antioxidant systems is age dependent. J Gerontol A Biol Sci Med Sci. 63:461–466. 2008. View Article : Google Scholar : PubMed/NCBI
6	Liu XP, Goldring CE, Wang HY, et al: Extract of Ginkgo biloba induces glutamate cysteine ligase catalytic subunit (GCLC). Phytother Res. 22:367–371. 2008.
7	Yuan J, Lu L, Zhang Z and Zhang S: Dietary intake of resveratrol enhances the adaptive immunity of aged rats. Rejuvenation Res. 15:507–515. 2012. View Article : Google Scholar : PubMed/NCBI
8	Pervaiz S and Holme AL: Resveratrol: its biologic targets and functional activity. Antioxid Redox Signal. 11:2851–2897. 2009. View Article : Google Scholar : PubMed/NCBI
9	Vang O, Ahmad N, Baile CA, et al: What is new for an old molecule? Systematic review and recommendations on the use of resveratrol. PLoS One. 6:e198812011. View Article : Google Scholar : PubMed/NCBI
10	Valenzano DR, Terzibasi E, Genade T, Cattaneo A, Domenici L and Cellerino A: Resveratrol prolongs lifespan and retards the onset of age-related markers in a short-lived vertebrate. Curr Biol. 16:296–300. 2006. View Article : Google Scholar : PubMed/NCBI
11	Evason K, Huang C, Yamben I, Covey DF and Kornfeld K: Anticonvulsant medications extend worm life-span. Science. 307:258–262. 2005. View Article : Google Scholar : PubMed/NCBI
12	Howitz KT, Bitterman KJ, Cohen HY, et al: Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan. Nature. 425:191–196. 2003. View Article : Google Scholar : PubMed/NCBI
13	Kang HL, Benzer S and Min KT: Life extension in Drosophila by feeding a drug. Proc Natl Acad Sci USA. 99:838–843. 2002.
14	Wood JG, Rogina B, Lavu S, Howitz K, Helfand SL, Tatar M and Sinclair D: Sirtuin activators mimic caloric restriction and delay ageing in metazoans. Nature. 430:686–689. 2004. View Article : Google Scholar : PubMed/NCBI
15	Lu SC: Regulation of glutathione synthesis. Mol Aspects Med. 30:42–59. 2009. View Article : Google Scholar
16	Franklin CC, Backos DS, Mohar I, White CC, Forman HJ and Kavanagh TJ: Structure, function, and post-translational regulation of the catalytic and modifier subunits of glutamate cysteine ligase. Mol Aspects Med. 30:86–98. 2009. View Article : Google Scholar : PubMed/NCBI
17	Hollander J, Bejma J, Ookawara T, Ohno H and Ji LL: Superoxide dismutase gene expression in skeletal muscle: fiber-specific effect of age. Mech Ageing Dev. 116:33–45. 2000. View Article : Google Scholar : PubMed/NCBI
18	Ji LL, Dillon D and Wu E: Alteration of antioxidant enzymes with aging in rat skeletal muscle and liver. Am J Physiol. 258:R918–R923. 1990.PubMed/NCBI
19	Kim HG, Hong SM, Kim SJ, et al: Age-related changes in the activity of antioxidant and redox enzymes in rats. Mol Cells. 16:278–284. 2003.PubMed/NCBI
20	Liu R and Choi J: Age-associated decline in gamma-glutamylcysteine synthetase gene expression in rats. Free Radic Biol Med. 28:566–574. 2000. View Article : Google Scholar : PubMed/NCBI
21	Mosoni L, Breuillé D, Buffière C, Obled C and Mirand PP: Age-related changes in glutathione availability and skeletal muscle carbonyl content in healthy rats. Exp Gerontol. 39:203–210. 2004. View Article : Google Scholar : PubMed/NCBI
22	Ohrloff C, Hockwin O, Olson R and Dickman S: Glutathione peroxidase, glutathione reductase and superoxide dismutase in the aging lens. Curr Eye Res. 3:109–115. 1984. View Article : Google Scholar : PubMed/NCBI
23	Zhu Y, Carvey PM and Ling Z: Age-related changes in glutathione and glutathione-related enzymes in rat brain. Brain Res. 1090:35–44. 2006. View Article : Google Scholar : PubMed/NCBI
24	Lambertucci RH, Levada-Pires AC, Rossoni LV, Curi R and Pithon-Curi TC: Effects of aerobic exercise training on antioxidant enzyme activities and mRNA levels in soleus muscle from young and aged rats. Mech Ageing Dev. 128:267–275. 2007. View Article : Google Scholar : PubMed/NCBI
25	Siu PM, Pistilli EE, Murlasits Z and Alway SE: Hindlimb unloading increases muscle content of cytosolic but not nuclear Id2 and p53 proteins in young adult and aged rats. J Appl Physiol (1985). 100:907–916. 2006.PubMed/NCBI
26	Kayani AC, Morton JP and McArdle A: The exercise-induced stress response in skeletal muscle: failure during aging. Appl Physiol Nutr Metab. 33:1033–1041. 2008. View Article : Google Scholar : PubMed/NCBI
27	Franco AA, Odom RS and Rando TA: Regulation of antioxidant enzyme gene expression in response to oxidative stress and during differentiation of mouse skeletal muscle. Free Radic Biol Med. 27:1122–1132. 1999. View Article : Google Scholar : PubMed/NCBI
28	Botta D, White CC, Vliet-Gregg P, et al: Modulating GSH synthesis using glutamate cysteine ligase transgenic and gene-targeted mice. Drug Metab Rev. 40:465–477. 2008. View Article : Google Scholar : PubMed/NCBI
29	Dalton TP, Dieter MZ, Yang Y, Shertzer HG and Nebert DW: Knockout of the mouse glutamate cysteine ligase catalytic subunit (Gclc) gene: embryonic lethal when homozygous, and proposed model for moderate glutathione deficiency when heterozygous. Biochem Biophys Res Commun. 279:324–329. 2000. View Article : Google Scholar
30	Yang Y, Dieter MZ, Chen Y, Shertzer HG, Nebert DW and Dalton TP: Initial characterization of the glutamate-cysteine ligase modifier subunit Gclm(−/−) knockout mouse. Novel model system for a severely compromised oxidative stress response. J Biol Chem. 277:49446–49452. 2002.PubMed/NCBI
31	Lee JI, Kang J and Stipanuk MH: Differential regulation of glutamate-cysteine ligase subunit expression and increased holoenzyme formation in response to cysteine deprivation. Biochem J. 393:181–190. 2006. View Article : Google Scholar