SB‑273005, an antagonist of αvβ3 integrin, reduces the production of Th2 cells and cytokine IL-10 in pregnant mice

  • Authors:
    • Shaojuan Wang
    • Jing Yang
    • Chongyang Wang
    • Qing Yang
    • Xiaoli Zhou
  • View Affiliations

  • Published online on: April 7, 2014     https://doi.org/10.3892/etm.2014.1667
  • Pages: 1677-1682
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Abstract

Pregnancy is associated with complex immunoreactions. In the present study, the effect of SB‑273005, an antagonist of αvβ3 integrin, on the alterations of T helper (Th) cells and their derived cytokines that occur during pregnancy was investigated in mice. Five non‑pregnant mice were used as a negative control. Mice were impregnated by co‑housing females and males at a ratio of 2:1 overnight and pregnancy was confirmed by the appearance of vaginal plugs the following morning. Day 1 (D1) pregnant mice were randomly divided into two groups (n=20) and were administered either dimethylsulfoxide (mock treatment) or SB‑273005 (3 mg/kg) by gavage at D3, D4 and D5. At D8, the levels of Th1 and Th2 cells and interleukin (IL)‑2 and IL‑10 in the spleen and peripheral blood were determined using flow cytometry and enzyme‑linked immunosorbent assay. Pregnancy significantly increased the ratio of Th2:Th1 cells in the spleen compared with that in non‑pregnant mice (P<0.01). However, this increase was significantly reduced by SB‑273005 (P<0.001). Furthermore, whilst pregnancy decreased Th1 cell‑produced IL‑2 levels and increased Th2 cell‑derived IL‑10 levels, SB‑273005 reversed both processes (P<0.05 for IL‑2; P<0.01 for IL‑10). The results from the present study demonstrated that pregnancy induces changes in the spleen, including a reduction of IL‑2 and an increase in IL‑10 production by Th1 and Th2 cells, respectively, as well as an upregulation of the Th2:Th1 ratio in the spleen. These immunological changes are reversed by SB‑273005, indicating an important role for αvβ3 integrin in mediating these immunological alterations.
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June-2014
Volume 7 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang S, Yang J, Wang C, Yang Q and Zhou X: SB‑273005, an antagonist of αvβ3 integrin, reduces the production of Th2 cells and cytokine IL-10 in pregnant mice. Exp Ther Med 7: 1677-1682, 2014
APA
Wang, S., Yang, J., Wang, C., Yang, Q., & Zhou, X. (2014). SB‑273005, an antagonist of αvβ3 integrin, reduces the production of Th2 cells and cytokine IL-10 in pregnant mice. Experimental and Therapeutic Medicine, 7, 1677-1682. https://doi.org/10.3892/etm.2014.1667
MLA
Wang, S., Yang, J., Wang, C., Yang, Q., Zhou, X."SB‑273005, an antagonist of αvβ3 integrin, reduces the production of Th2 cells and cytokine IL-10 in pregnant mice". Experimental and Therapeutic Medicine 7.6 (2014): 1677-1682.
Chicago
Wang, S., Yang, J., Wang, C., Yang, Q., Zhou, X."SB‑273005, an antagonist of αvβ3 integrin, reduces the production of Th2 cells and cytokine IL-10 in pregnant mice". Experimental and Therapeutic Medicine 7, no. 6 (2014): 1677-1682. https://doi.org/10.3892/etm.2014.1667