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Article

Inhibitory effect of gene combination in a mouse model of colon cancer with liver metastasis

  • Authors:
    • Tong Du
    • Hongxin Niu
  • View Affiliations / Copyright

    Affiliations: Department of Psychology, Affiliated Hospital of Shandong Academy of Medical Sciences, Jinan, Shandong 250031, P.R. China
  • Pages: 913-918
    |
    Published online on: June 25, 2014
       https://doi.org/10.3892/etm.2014.1809
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Abstract

The aim of the present study was to establish an animal liver metastasis model with human colon cancer and investigate the inhibitory effect of the wild type (WT) p53 gene combined with thymidine kinase/ganciclovir (TK/GCV) and cytosine deaminase/5‑fluorocytosine (CD/5‑FC) systems on liver metastasis of colon cancer. A nude mouse liver metastasis model with human colon cancer was established via a spleen cultivation method. A total of 32 nude mice were randomly divided into four groups, each group with eight mice. Group 1 mice received splenic injections of SW480 cells (control group), while group 2 mice were injected with SW480/p53 cells in the spleen. Group 3 mice were administered splenic injections of SW480/TK‑CD cells, and GCV and 5‑FC were injected into the abdominal cavity. Finally, group 4 mice received splenic injections of SW480/p53 cells mixed in equal proportion with SW480/TK‑CD cells, as well as GCV and 5‑FC injections in the abdominal cavity. These cells described were constructed in our laboratory and other laboratories. The number of liver metastatic tumors, the liver metastasis rate, conventional pathology, electron microscopy and other indicators in the nude mice of each group were compared and observed. The nude mouse liver metastasis model with human colon cancer was successfully established; the liver metastasis rate of the control group was 100%. The results demonstrated that the rate of liver metastasis in the nude mice in each treatment group decreased, as well as the average number of liver metastatic tumors. Furthermore, the effect of the treatment group with genetic combination (group 4) was the most effective, demonstrating that WTp53 had a synergistic effect with TK/GCV and CD/5‑FC. Therefore, the present study successfully established a mouse model of liver metastasis with colon cancer by injecting human colon cancer cells in the spleen. Combined gene therapy was shown to have a synergistic effect, which effectively inhibited the formation of liver metastasis from colon cancer.
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1 

Kruse J, von Bernstorff W, Evert K, et al: Macrophages promote tumour growth and liver metastasis in an orthotopic syngeneic mouse model of colon cancer. Int J Colorectal Dis. 28:1337–1349. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Schiesser M, Chen JW, Maddern GJ and Padbury RT: Perioperative morbidity affects long-term survival in patients following liver resection for colorectal metastases. J Gastrointest Surg. 12:1054–1060. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Huang X, Zou Y, Lian L, et al: Changes of T cells and cytokines TGF-β1 and IL-10 in mice during liver metastasis of colon carcinoma: implications for liver anti-tumor immunity. J Gastrointestinal Surg. 17:1283–1291. 2013.

4 

Min BS, Kim NK, Jeong HC and Chung HC: High levels of serum VEGF and TIMP-1 are correlated with colon cancer liver metastasis and intrahepatic recurrence after liver resection. Oncology Lett. 4:123–130. 2012.PubMed/NCBI

5 

Ceauşu RA, Cîmpean AM, Gaje P, Gurzu S, Jung I and Raica M: CD105/Ki67 double immunostaining expression in liver metastasis from colon carcinoma. Rom J Morphol Embryol. 52:613–616. 2011.PubMed/NCBI

6 

Yoshida D, Ikeda Y, Waki K, Shirabe K, Kakeji Y, Tsujitani S and Maehara Y: Different incidence of synchronous liver metastasis between proximal and distal colon cancer. Surg Today. 42:426–430. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Rees M, Tekkis PP, Welsh FK, O’Rourke T and John TG: Evaluation of long-term survival after hepatic resection for metastatic colorectal cancer: A multifactorial model of 929 patients. Ann Surg. 247:125–135. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Zhang SX, Zhang SL and Lan XP: The establishment of a subcutaneously transplanted tumor model with human colon cancer cell line SW480 in nude mice. Military Medical Journal of Southeast China. 14:108–110. 2012.(In Chinese).

9 

Niu HX, He QS, Leng YD and Liu YZ: The establishment of the model of hepatic metastasis with human colon cancer cell line SW480 in nude mice. Chin J Curr Adv Gen Surg. 10:540–542. 2007.(In Chinese).

10 

Metildi CA, Kaushal S, Snyder CS, Hoffman RM and Bouvet M: Fluorescence-guided surgery of human colon cancer increases complete resection resulting in cures in an orthotopic nude mouse model. J Surg Res. 179:87–93. 2012. View Article : Google Scholar

11 

Schoffelen R, van der Graaf WT, Sharkey RM, et al: Quantitative immuno-SPECT monitoring of pretargeted radioimmunotherapy with a bispecific antibody in an intraperitoneal nude mouse model of human colon cancer. J Nucl Med. 53:1926–1932. 2012. View Article : Google Scholar

12 

Rada T, Carvalho PP, Santos TC, Castro AG, Reis RL and Gomes ME: Chondrogenic potential of two hASCs subpopulations loaded onto gellan gum hydrogel evaluated in a nude mice model. Curr Stem Cell Res Ther. 8:357–364. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Yang L, Zhou J, Ma Q, et al: Knockdown of PPARδ gene promotes the growth of colon cancer and reduces the sensitivity to bevacizumab in nude mice model. PloS One. 8:e607152013.

14 

Okal A, Mossalam M, Matissek KJ, Dixon AS, Moos PJ and Lim CS: A chimeric p53 evades mutant p53 transdominant inhibition in cancer cells. Mol Pharm. 10:3922–3933. 2013.PubMed/NCBI

15 

Khoury MP and Bourdon JC: The isoform of the p53 protein. Cold Spring Harb Perspect Biol. 2:a0009272010. View Article : Google Scholar : PubMed/NCBI

16 

Ladelfa MF, Peche LY, Toledo MF, Laiseca JE, Schneider C and Martín M: Tumor-specific MAGE proteins as regulators of p53 function. Cancer Lett. 325:11–17. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Menendez D, Nguyen TA, Freudenberg JM, Mathew VJ, Anderson CW, Jothi R and Resnick MA: Diverse stresses dramatically alter genome-wide p53 binding and transactivation landscape in human cancer cells. Nucleic Acids Res. 41:7286–7301. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Liu X, Wilcken R, Joerger AC, Chuckowree IS, Amin J, Spencer J and Fersht AR: Small molecule induced reactivation of mutant p53 in cancer cells. Nucleic Acids Res. 41:6034–6044. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Dong XY, Wang WQ, Zhao Y, et al: Antibody-directed double suicide gene therapy targeting of MUC1-positive leukemia cells in vitro and in vivo. Curr Gene Ther. 13:346–357. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Chen Y, Wang G, Kong D, et al: Double-targeted and double-enhanced suicide gene therapy mediated by generation 5 polyamidoamine dendrimers for prostate cancer. Mol Carcinog. 52:237–246. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Li LQ, Shen F, Xu XY, Zhang H, Yang XF and Liu WG: Gene therapy with HSV1-sr39TK/GCV exhibits a stronger therapeutic efficacy than HSV1-TK/GCV in rat C6 glioma cells. ScientificWorldJournal. 2013:9513432013.PubMed/NCBI

22 

Lu Z, Zhang TY, Han MM, et al: Antitumor activity of the recombinant rClone30-CD/5-FC system. Yao Xue Xue Bao. 48:261–268. 2013.(In Chinese).

23 

Chai LP, Wang ZF, Liang WY, Chen L, Chen D, Wang AX and Zhang ZQ: In vitro and in vivo effect of 5-FC combined gene therapy with TNF-α and CD suicide gene on human laryngeal carcinoma cell line Hep-2. PloS One. 8:e611362013.

24 

Torimura T, Ueno T, Taniguchi E, et al: Interaction of endothelial progenitor cells expressing cytosine deaminase in tumor tissues and 5-fluorocytosine administration suppresses growth of 5-fluorouracil-sensitive liver cancer in mice. Cancer Sci. 103:542–548. 2011. View Article : Google Scholar

25 

Niu HX, Du T, Xu ZF, Zhang XK and Wang RG: Role of wild type p53 and double suicide genes in interventional therapy of liver cancer in rabbits. Acta Cir Bras. 27:522–528. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Pasquale EB: Eph receptors and ephrins in cancer: bidirectional signaling and beyond. Nat Rev Cancer. 10:165–180. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Du T and Niu H: Inhibitory effect of gene combination in a mouse model of colon cancer with liver metastasis. Exp Ther Med 8: 913-918, 2014.
APA
Du, T., & Niu, H. (2014). Inhibitory effect of gene combination in a mouse model of colon cancer with liver metastasis. Experimental and Therapeutic Medicine, 8, 913-918. https://doi.org/10.3892/etm.2014.1809
MLA
Du, T., Niu, H."Inhibitory effect of gene combination in a mouse model of colon cancer with liver metastasis". Experimental and Therapeutic Medicine 8.3 (2014): 913-918.
Chicago
Du, T., Niu, H."Inhibitory effect of gene combination in a mouse model of colon cancer with liver metastasis". Experimental and Therapeutic Medicine 8, no. 3 (2014): 913-918. https://doi.org/10.3892/etm.2014.1809
Copy and paste a formatted citation
x
Spandidos Publications style
Du T and Niu H: Inhibitory effect of gene combination in a mouse model of colon cancer with liver metastasis. Exp Ther Med 8: 913-918, 2014.
APA
Du, T., & Niu, H. (2014). Inhibitory effect of gene combination in a mouse model of colon cancer with liver metastasis. Experimental and Therapeutic Medicine, 8, 913-918. https://doi.org/10.3892/etm.2014.1809
MLA
Du, T., Niu, H."Inhibitory effect of gene combination in a mouse model of colon cancer with liver metastasis". Experimental and Therapeutic Medicine 8.3 (2014): 913-918.
Chicago
Du, T., Niu, H."Inhibitory effect of gene combination in a mouse model of colon cancer with liver metastasis". Experimental and Therapeutic Medicine 8, no. 3 (2014): 913-918. https://doi.org/10.3892/etm.2014.1809
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