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Article

DNA methylation of microRNA‑375 in impaired glucose tolerance

  • Authors:
    • Xiaoli Wang
    • Xiangyun Chang
    • Jun Li
    • Liang Yin
    • Kan Sun
  • View Affiliations / Copyright

    Affiliations: Departments of Endocrinology and Metabolism, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, P.R. China
  • Pages: 775-780
    |
    Published online on: June 30, 2014
       https://doi.org/10.3892/etm.2014.1816
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Abstract

In the present study, the expression levels and DNA methylation status of microRNA (miRNA)‑375 in patients with impaired glucose tolerance (IGT) and type 2 diabetes mellitus (T2DM) were analyzed and the role of DNA methylation of miRNA‑375 in the pathogenesis of T2DM was investigated. Compared with the miR‑375 levels in patients with normal glucose tolerance (NGT; n=53), the samples from patients with IGT (n=44) exhibited downregulation of miR‑375, while those from patients with T2DM (n=54) exhibited upregulation of miR‑375 in the plasma. Additionally, the samples from patients with IGT were observed to be hypermethylated compared with those from patients with T2DM and NGT (P=0.042). Analysis of three CpG units (CpG1.2, CpG20 and CpG25.26.27) from 17 CpG sites (between -990 and ‑1,258 bp, relative to the transcription start site) revealed higher methylation levels in patients with IGT compared with those in patients with NGT (P<0.05). The methylation of two CpG units (CpG1.2 and CpG25.26.27) was higher in patients with IGT than in the patients with T2DM (P<0.05). Thus, the present study demonstrated that the miR‑375 promoter was hypermethylated and the levels of miR‑375 in the plasma were downregulated in the patients with IGT. DNA hypomethylation may have an important role in the regulation of miR‑375 expression and may contribute to the pathogenesis of T2DM.
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1 

DeFronzo RA: Banting Lecture. From the triumvirate to the ominous octet: a new paradigm for the treatment of type 2 diabetes mellitus. Diabetes. 58:773–795. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Ferrannini E, Gastaldelli A, Miyazaki Y, Matsuda M, Mari A and DeFronzo RA: β-Cell function in subjects spanning the range from normal glucose tolerance to overt diabetes mellitus: a new analysis. J Clin Endocrinol Metab. 90:493–500. 2005.

3 

Abdul-Ghani MA, Tripathy D and DeFronzo RA: Contribution of β-cell dysfunction and insulin resistance to the pathogenesis of impaired glucose tolerance and impaired fasting glucose. Diabetes Care. 29:1130–1139. 2006.

4 

Abdul-Ghani MA, Jenkinson CP, Richardson DK, Tripathy D and DeFronzo RA: Insulin secretion and insulin action in subjects with impaired fasting glucose and impaired glucose tolerance: results from the Veterans Administration Genetic Epidemiology Study. Diabetes. 55:1430–1435. 2006. View Article : Google Scholar

5 

Weyer C, Tataranni PA, Bogardus C and Pratley RE: Insulin resistance and insulin secretory dysfunction are independent predictors of worsening of glucose tolerance during each stage of type 2 diabetes development. Diabetes Care. 24:89–94. 2001. View Article : Google Scholar

6 

Jallut D, Golay A, Munger R, Frascarolo P, Schutz Y, Jéquier E and Felber JP: Impaired glucose tolerance and diabetes in obesity: a 6-year follow-up study of glucose metabolism. Metabolism. 39:1068–1075. 1990.PubMed/NCBI

7 

Altshuler D, Hirschhorn JN, Klannemark M, et al: The common PPARgamma Pro12Ala polymorphism is associated with decreased risk of type 2 diabetes. Nat Genet. 26:76–80. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Rissanen J, Markkanen A, Kärkkäinen P, et al: Sulfonylurea receptor 1 gene variants are associated with gestational diabetes and type 2 diabetes but not with altered secretion of insulin. Diabetes Care. 23:70–73. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Hara K, Okada T, Tobe K, et al: The Pro12Ala polymorphism in PPARγ2 may confer resistance to type 2 diabetes. Biochem Biophys Res Commu. 271:212–216. 2000.

10 

Chang TC and Mendell JT: microRNAs in vertebrate physiology and human disease. Annu Rev Genomics Hum Genet. 8:215–239. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Poy MN, Eliasson L, Krutzfeldt J, et al: A pancreatic islet-specific microRNA regulates insulin secretion. Nature. 432:226–230. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Baroukh N, Ravier MA, Loder MK, et al: MicroRNA-124a regulates Foxa2 expression and intracellular signaling in pancreatic β-cell lines. J Biol Chem. 282:19575–19588. 2007.PubMed/NCBI

13 

Joglekar MV, Parekh VS, Mehta S, Bhonde RR and Hardikar AA: MicroRNA profiling of developing and regenerating pancreas reveal post-transcriptional regulation of neurogenin3. Dev Biol. 311:603–612. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Plaisance V, Abderrahmani A, Perret-Menoud V, Jacquemin P, Lemaigre F and Regazzi R: MicroRNA-9 controls the expression of Granuphilin/Slp4 and the secretory response of insulin-producing cells. J Biol Chem. 281:26932–26942. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Tang X, Muniappan L, Tang G and Ozcan S: Identification of glucose-regulated miRNAs from pancreatic β cells reveals a role for miR-30d in insulin transcription. RNA. 15:287–293. 2009.PubMed/NCBI

16 

Lynn FC, Skewes-Cox P, Kosaka Y, McManus MT, Harfe BD and German MS: MicroRNA expression is required for pancreatic islet cell genesis in the mouse. Diabetes. 56:2938–2945. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Temple IK, Gardner RJ, Mackay DJ, Barber JC, Robinson DO and Shield JP: Transient neonatal diabetes: widening the understanding of the etiopathogenesis of diabetes. Diabetes. 49:1359–1366. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Report of a WHO Consultation: Definition, Diagnosis and Classification of Diabetes Mellitus and its complications: Report of a WHO Consultation 1999. Part 1: Diagnosis and classification of diabetes mellitus. 1–65. 1999.

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001.

20 

Poy MN, Hausser J, Trajkovski M, et al: miR-375 maintains normal pancreatic alpha- and beta-cell mass. Proc Natl Acad Sci USA. 106:5813–5818. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Xia HQ, Pan Y, Peng J and Lu GX: Over-expression of miR375 reduces glucose-induced insulin secretion in Nit-1 cells. Mol Bio Rep. 38:3061–3065. 2011. View Article : Google Scholar : PubMed/NCBI

22 

El Ouaamari A, Baroukh N, Martens GA, Lebrun P, Pipeleers D and van Obberghen E: miR-375 targets 3′-phosphoinositide-dependent protein kinase-1 and regulates glucose-induced biological responses in pancreatic β-cells. Diabetes. 57:2708–2717. 2008.

23 

de Souza Rocha Simonini P, Breiling A, Gupta N, et al: Epigenetically deregulated microRNA-375 is involved in a positive feedback loop with estrogen receptor alpha in breast cancer cells. Cancer Res. 70:9175–9184. 2010.PubMed/NCBI

24 

Ding L, Xu Y, Zhang W, et al: MiR-375 frequently downregulated in gastric cancer inhibits cell proliferation by targeting JAK2. Cell Res. 20:784–793. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Sun K, Chang XY, Yin L, et al: Expression and DNA methylation status of microRNA-375 in patients with type 2 diabetes mellitus. Mol Med Rep. 9:967–972. 2014.PubMed/NCBI

26 

Reid G, Métivier R, Lin CY, et al: Multiple mechanisms induce transcriptional silencing of a subset of genes, including oestrogen receptor alpha, in response to deacetylase inhibition by valproic acid and trichostatin A. Oncogene. 24:4894–4907. 2005. View Article : Google Scholar

27 

Kangaspeska S, Stride B, Métivier R, et al: Transient cyclical methylation of promoter DNA. Nature. 452:112–115. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Métivier R, Gallais R, Tiffoche C, et al: Cyclical DNA methylation of a transcriptionally active promoter. Nature. 452:45–50. 2008.PubMed/NCBI

29 

Maier S and Olek A: Diabetes: a candidate disease for efficient DNA methylation profiling. J Nutr. 132(Suppl 8): 2440S–2443S. 2002.PubMed/NCBI

30 

Barrès R, Osler ME, Yan J, et al: Non-CpG methylation of the PGC-1alpha promoter through DNMT3B controls mitochondrial density. Cell Metab. 10:189–198. 2009.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Chang X, Li J, Yin L and Sun K: DNA methylation of microRNA‑375 in impaired glucose tolerance. Exp Ther Med 8: 775-780, 2014.
APA
Wang, X., Chang, X., Li, J., Yin, L., & Sun, K. (2014). DNA methylation of microRNA‑375 in impaired glucose tolerance. Experimental and Therapeutic Medicine, 8, 775-780. https://doi.org/10.3892/etm.2014.1816
MLA
Wang, X., Chang, X., Li, J., Yin, L., Sun, K."DNA methylation of microRNA‑375 in impaired glucose tolerance". Experimental and Therapeutic Medicine 8.3 (2014): 775-780.
Chicago
Wang, X., Chang, X., Li, J., Yin, L., Sun, K."DNA methylation of microRNA‑375 in impaired glucose tolerance". Experimental and Therapeutic Medicine 8, no. 3 (2014): 775-780. https://doi.org/10.3892/etm.2014.1816
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Chang X, Li J, Yin L and Sun K: DNA methylation of microRNA‑375 in impaired glucose tolerance. Exp Ther Med 8: 775-780, 2014.
APA
Wang, X., Chang, X., Li, J., Yin, L., & Sun, K. (2014). DNA methylation of microRNA‑375 in impaired glucose tolerance. Experimental and Therapeutic Medicine, 8, 775-780. https://doi.org/10.3892/etm.2014.1816
MLA
Wang, X., Chang, X., Li, J., Yin, L., Sun, K."DNA methylation of microRNA‑375 in impaired glucose tolerance". Experimental and Therapeutic Medicine 8.3 (2014): 775-780.
Chicago
Wang, X., Chang, X., Li, J., Yin, L., Sun, K."DNA methylation of microRNA‑375 in impaired glucose tolerance". Experimental and Therapeutic Medicine 8, no. 3 (2014): 775-780. https://doi.org/10.3892/etm.2014.1816
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