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Article

Preclinical humanized mouse model with ectopic ovarian tissues

  • Authors:
    • Shilong Fu
    • Jue Wang
    • Wu Sun
    • Yi Xu
    • Xiaoyu Zhou
    • Wenjun Cheng

  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Clinical Pathology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Blood Transfusion, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
  • Pages: 742-746
    |
    Published online on: July 1, 2014
       https://doi.org/10.3892/etm.2014.1819
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Abstract

The aim of the present study was to establish human ovarian stroma within the mouse subcutaneously, in order for the resulting stroma to serve as a useful preclinical tool to study the progression of human ovarian cancer in a humanized ovarian microenvironment. Normal human ovarian tissues were subcutaneously implanted into severe combined immunodeficient (SCID) mice and then the implants were identified by immunohistochemistry. The implants became vascularized and retained their original morphology for about 4 weeks following implantation. Immunohistochemical staining for cytokeratin-7 confirmed the ovarian origin of the epithelial cells. CD34 staining demonstrated human-derived vessels. Positive estrogen receptor and partially‑positive progesterone receptor staining indicated the estrogen and progesterone dependence of the implants. Only vascular pericytes expressed α-smooth muscle actin, indicating the normal ovarian origin of the xenografts. Human ovarian tissue successfully survived in SCID mice and retained its original properties. This humanized mouse model may be used as preclinical tool to investigate ovarian cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Fu S, Wang J, Sun W, Xu Y, Zhou X and Cheng W: Preclinical humanized mouse model with ectopic ovarian tissues. Exp Ther Med 8: 742-746, 2014.
APA
Fu, S., Wang, J., Sun, W., Xu, Y., Zhou, X., & Cheng, W. (2014). Preclinical humanized mouse model with ectopic ovarian tissues. Experimental and Therapeutic Medicine, 8, 742-746. https://doi.org/10.3892/etm.2014.1819
MLA
Fu, S., Wang, J., Sun, W., Xu, Y., Zhou, X., Cheng, W."Preclinical humanized mouse model with ectopic ovarian tissues". Experimental and Therapeutic Medicine 8.3 (2014): 742-746.
Chicago
Fu, S., Wang, J., Sun, W., Xu, Y., Zhou, X., Cheng, W."Preclinical humanized mouse model with ectopic ovarian tissues". Experimental and Therapeutic Medicine 8, no. 3 (2014): 742-746. https://doi.org/10.3892/etm.2014.1819
Copy and paste a formatted citation
x
Spandidos Publications style
Fu S, Wang J, Sun W, Xu Y, Zhou X and Cheng W: Preclinical humanized mouse model with ectopic ovarian tissues. Exp Ther Med 8: 742-746, 2014.
APA
Fu, S., Wang, J., Sun, W., Xu, Y., Zhou, X., & Cheng, W. (2014). Preclinical humanized mouse model with ectopic ovarian tissues. Experimental and Therapeutic Medicine, 8, 742-746. https://doi.org/10.3892/etm.2014.1819
MLA
Fu, S., Wang, J., Sun, W., Xu, Y., Zhou, X., Cheng, W."Preclinical humanized mouse model with ectopic ovarian tissues". Experimental and Therapeutic Medicine 8.3 (2014): 742-746.
Chicago
Fu, S., Wang, J., Sun, W., Xu, Y., Zhou, X., Cheng, W."Preclinical humanized mouse model with ectopic ovarian tissues". Experimental and Therapeutic Medicine 8, no. 3 (2014): 742-746. https://doi.org/10.3892/etm.2014.1819
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