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Article Open Access

Expression of serotonin receptors in human lower esophageal sphincter

  • Authors:
    • He-Fei Li
    • Jun-Feng Liu
    • Ke Zhang
    • Yong Feng
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China
  • Pages: 49-54
    |
    Published online on: November 4, 2014
       https://doi.org/10.3892/etm.2014.2050
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Abstract

Serotonin (5-HT) is a neurotransmitter and vasoactive amine that is involved in the regulation of a large number of physiological functions. The wide variety of 5-HT-mediated functions is due to the existence of different classes of serotonergic receptors in the mammalian gastrointestinal tract and nervous system. The aim of this study was to explore the expression of multiple types of 5-HT receptor (5-HT1AR, 5-HT2AR, 5-HT3AR, 5-HT4R, 5-HT5AR, 5-HT6R and 5-HT7R) in sling and clasp fibers from the human lower esophageal sphincter (LES). Muscle strips of sling and clasp fibers from the LES were obtained from patients undergoing esophagogastrectomy, and circular muscle strips from the esophagus and stomach were used as controls. Reverse transcription‑polymerase chain reaction (RT-PCR), quantitative PCR and western blotting were used to investigate the expression of the various 5-HT receptor types. Messenger RNA for all seven 5-HT receptor types was identified in the sling and clasp fibers of the LES. At the mRNA level, the expression levels were highest for 5-HT3AR and 5-HT4R, and lowest for 5-HT5AR, 5-HT6R and 5-HT7R. At the protein level, the expression levels were highest for 5-HT3AR and 5-HT4R, followed by 5-HT1AR and 5-HT2AR; 5-HT7R was also detected at a low level. The expression of 5-HT5AR and 5-HT6R proteins was not confirmed. The results indicate that a variety of 5-HT receptor types can be detected in the human LES and probably contribute to LES function.
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Copy and paste a formatted citation
Spandidos Publications style
Li H, Liu J, Zhang K and Feng Y: Expression of serotonin receptors in human lower esophageal sphincter. Exp Ther Med 9: 49-54, 2015.
APA
Li, H., Liu, J., Zhang, K., & Feng, Y. (2015). Expression of serotonin receptors in human lower esophageal sphincter. Experimental and Therapeutic Medicine, 9, 49-54. https://doi.org/10.3892/etm.2014.2050
MLA
Li, H., Liu, J., Zhang, K., Feng, Y."Expression of serotonin receptors in human lower esophageal sphincter". Experimental and Therapeutic Medicine 9.1 (2015): 49-54.
Chicago
Li, H., Liu, J., Zhang, K., Feng, Y."Expression of serotonin receptors in human lower esophageal sphincter". Experimental and Therapeutic Medicine 9, no. 1 (2015): 49-54. https://doi.org/10.3892/etm.2014.2050
Copy and paste a formatted citation
x
Spandidos Publications style
Li H, Liu J, Zhang K and Feng Y: Expression of serotonin receptors in human lower esophageal sphincter. Exp Ther Med 9: 49-54, 2015.
APA
Li, H., Liu, J., Zhang, K., & Feng, Y. (2015). Expression of serotonin receptors in human lower esophageal sphincter. Experimental and Therapeutic Medicine, 9, 49-54. https://doi.org/10.3892/etm.2014.2050
MLA
Li, H., Liu, J., Zhang, K., Feng, Y."Expression of serotonin receptors in human lower esophageal sphincter". Experimental and Therapeutic Medicine 9.1 (2015): 49-54.
Chicago
Li, H., Liu, J., Zhang, K., Feng, Y."Expression of serotonin receptors in human lower esophageal sphincter". Experimental and Therapeutic Medicine 9, no. 1 (2015): 49-54. https://doi.org/10.3892/etm.2014.2050
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