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Article

Inhibitory effects of B-cell lymphoma 2 on the vasculogenic mimicry of hypoxic human glioma cells

  • Authors:
    • Jianwen Li
    • Yiquan Ke
    • Min Huang
    • Shuyun Huang
    • Yiming Liang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Neurosurgery Institute, Key Laboratory on Brain Function Repair and Regeneration of Guangdong, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510282, P.R. China
  • Pages: 977-981
    |
    Published online on: December 30, 2014
       https://doi.org/10.3892/etm.2014.2162
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Abstract

The aim of this study was to investigate the mechanisms and effects of B‑cell lymphoma 2 (Bcl‑2) on the vasculogenic mimicry (VM) of human glioma cells. U87 cells were cultured under hypoxic conditions and then divided into four groups: Control, 3‑(5‑hydroxymethyl‑2‑furyl)‑1‑benzylindazole (YC‑1), ABT‑737 and YC‑1 + ABT‑737. These groups were treated with the corresponding simulators. The expression of hypoxia‑inducible factor‑1α (HIF‑1α), matrix metalloproteinase (MMP)‑2, MMP‑14 and Bcl‑2 in each group was determined using a reverse transcription‑quantitative polymerase chain reaction and western blot analysis. Compared with that in the control group, the mRNA and protein expression of MMP‑2, MMP‑14 and Bcl‑2 in the YC‑1 and ABT‑737 groups was significantly reduced. The expression of HIF‑1α, however, was only significantly reduced in the YC‑1 group (P<0.05). Compared with those in the YC‑1 + ABT‑737 group, the expression levels of the four proteins in the YC‑1 and ABT‑737 groups were not significantly different, with the exception of the expression of HIF‑1α in the ABT‑737 group, which was significantly enhanced (P<0.05). The mRNA expression levels of HIF‑1α, MMP‑2 and MMP‑14 in the YC‑1 group were significantly different from those in the ABT‑737 group (P<0.01); however, no significant difference was observed in the expression of Bcl‑2. In conclusion, Bcl‑2 may be an important factor in the VM formation of human malignant glioma U87 cells under hypoxic conditions. Certain functions of Bcl‑2 may be attributed to the HIF‑1α‑MMP‑2‑MMP‑14‑VM channel, whereas other functions may be independent of the channel.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, Ke Y, Huang M, Huang S and Liang Y: Inhibitory effects of B-cell lymphoma 2 on the vasculogenic mimicry of hypoxic human glioma cells. Exp Ther Med 9: 977-981, 2015.
APA
Li, J., Ke, Y., Huang, M., Huang, S., & Liang, Y. (2015). Inhibitory effects of B-cell lymphoma 2 on the vasculogenic mimicry of hypoxic human glioma cells. Experimental and Therapeutic Medicine, 9, 977-981. https://doi.org/10.3892/etm.2014.2162
MLA
Li, J., Ke, Y., Huang, M., Huang, S., Liang, Y."Inhibitory effects of B-cell lymphoma 2 on the vasculogenic mimicry of hypoxic human glioma cells". Experimental and Therapeutic Medicine 9.3 (2015): 977-981.
Chicago
Li, J., Ke, Y., Huang, M., Huang, S., Liang, Y."Inhibitory effects of B-cell lymphoma 2 on the vasculogenic mimicry of hypoxic human glioma cells". Experimental and Therapeutic Medicine 9, no. 3 (2015): 977-981. https://doi.org/10.3892/etm.2014.2162
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Ke Y, Huang M, Huang S and Liang Y: Inhibitory effects of B-cell lymphoma 2 on the vasculogenic mimicry of hypoxic human glioma cells. Exp Ther Med 9: 977-981, 2015.
APA
Li, J., Ke, Y., Huang, M., Huang, S., & Liang, Y. (2015). Inhibitory effects of B-cell lymphoma 2 on the vasculogenic mimicry of hypoxic human glioma cells. Experimental and Therapeutic Medicine, 9, 977-981. https://doi.org/10.3892/etm.2014.2162
MLA
Li, J., Ke, Y., Huang, M., Huang, S., Liang, Y."Inhibitory effects of B-cell lymphoma 2 on the vasculogenic mimicry of hypoxic human glioma cells". Experimental and Therapeutic Medicine 9.3 (2015): 977-981.
Chicago
Li, J., Ke, Y., Huang, M., Huang, S., Liang, Y."Inhibitory effects of B-cell lymphoma 2 on the vasculogenic mimicry of hypoxic human glioma cells". Experimental and Therapeutic Medicine 9, no. 3 (2015): 977-981. https://doi.org/10.3892/etm.2014.2162
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