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Article

Combination of C-X-C motif chemokine 9 and C-X-C motif chemokine 10 antibodies with FTY720 prolongs the survival of cardiac retransplantation allografts in a mouse model

  • Authors:
    • Teng Ma
    • Jiacheng Xu
    • Jiawei Zhuang
    • Xiaobiao Zhou
    • Lianfeng Lin
    • Zhonggui Shan
    • Zhongquan Qi
  • View Affiliations / Copyright

    Affiliations: Department of Cardiac Surgery, The First Affiliated Hospital, Xiamen University, Xiamen, Fujian 361003, P.R. China, Organ Transplantation Institute, Medical College, Xiamen University, Xiamen, Fujian 361005, P.R. China
  • Pages: 1006-1012
    |
    Published online on: January 22, 2015
       https://doi.org/10.3892/etm.2015.2204
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Abstract

The upregulation of chemokine genes and the subsequent T‑lymphocyte recruitment to the graft are early events in the development of acute cardiac transplant rejection or cardiac allograft vasculopathy. In the present study, a combined immunosuppressive regimen of C-X-C motif chemokine 9 (CXCL9) antibody (Ab), CXCL10 Ab and FTY720 was used in order to reduce the infiltration of memory T lymphocytes and prolong graft survival in a retransplantation murine model. BALB/c donor hearts were transplanted heterotopically into C57BL/6 mice at day 28 after skin transplantation. The mice were divided into four groups: i) Control (normal saline), ii) CXCL9 Ab and CXCL10 Ab [150 µg; once daily (qd); intraperitoneal (ip)], iii) FTY720 (0.2 mg/day; qd; ip) and iv) combined (2 mg/kg/day; qd; ip). Measurements of the median survival time of the cardiac grafts, histological examination, reverse transcription‑quantitative polymerase chain reaction analysis, enzyme‑linked immunosorbent assay and a mixed lymphocyte reaction were performed. The median graft survival time of the combined group was prolonged (9.3 days) compared with that of the control group (3.5 days) (P<0.001). Histological examination revealed that the combined treatment group graft rejection pathological score was 0.50, while the control group score was 3.62 (P<0.001). In addition, the gene expression level of interleukin (IL)‑2 was significantly lower and the levels of IL‑10 and transforming growth factor‑β (TGF‑β) were significantly higher in the combined group compared with those in the control group (P<0.001). Furthermore, the serum concentration levels of IL‑2 and interferon‑γ (IFN‑γ) were significantly lower (P<0.001) and the concentration of IL‑10 was significantly higher (P<0.05) in the combined group compared with those in the control group. In the mixed lymphocyte reaction, T‑cell proliferation was found to be significantly lower in the combined treatment group than that in the control group (P<0.001). In conclusion, treatment with CXCL9 Ab and CXCL10 Ab or FTY720 reduced the graft infiltration of inflammatory cells, inhibited T‑cell proliferation and prolonged graft survival. The combined treatment regimen of CXCL9 Ab, CXCL10 Ab and FTY720 was found to significantly reduce the infiltration of inflammatory cells in the graft and prolong graft survival.
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Copy and paste a formatted citation
Spandidos Publications style
Ma T, Xu J, Zhuang J, Zhou X, Lin L, Shan Z and Qi Z: Combination of C-X-C motif chemokine 9 and C-X-C motif chemokine 10 antibodies with FTY720 prolongs the survival of cardiac retransplantation allografts in a mouse model. Exp Ther Med 9: 1006-1012, 2015.
APA
Ma, T., Xu, J., Zhuang, J., Zhou, X., Lin, L., Shan, Z., & Qi, Z. (2015). Combination of C-X-C motif chemokine 9 and C-X-C motif chemokine 10 antibodies with FTY720 prolongs the survival of cardiac retransplantation allografts in a mouse model. Experimental and Therapeutic Medicine, 9, 1006-1012. https://doi.org/10.3892/etm.2015.2204
MLA
Ma, T., Xu, J., Zhuang, J., Zhou, X., Lin, L., Shan, Z., Qi, Z."Combination of C-X-C motif chemokine 9 and C-X-C motif chemokine 10 antibodies with FTY720 prolongs the survival of cardiac retransplantation allografts in a mouse model". Experimental and Therapeutic Medicine 9.3 (2015): 1006-1012.
Chicago
Ma, T., Xu, J., Zhuang, J., Zhou, X., Lin, L., Shan, Z., Qi, Z."Combination of C-X-C motif chemokine 9 and C-X-C motif chemokine 10 antibodies with FTY720 prolongs the survival of cardiac retransplantation allografts in a mouse model". Experimental and Therapeutic Medicine 9, no. 3 (2015): 1006-1012. https://doi.org/10.3892/etm.2015.2204
Copy and paste a formatted citation
x
Spandidos Publications style
Ma T, Xu J, Zhuang J, Zhou X, Lin L, Shan Z and Qi Z: Combination of C-X-C motif chemokine 9 and C-X-C motif chemokine 10 antibodies with FTY720 prolongs the survival of cardiac retransplantation allografts in a mouse model. Exp Ther Med 9: 1006-1012, 2015.
APA
Ma, T., Xu, J., Zhuang, J., Zhou, X., Lin, L., Shan, Z., & Qi, Z. (2015). Combination of C-X-C motif chemokine 9 and C-X-C motif chemokine 10 antibodies with FTY720 prolongs the survival of cardiac retransplantation allografts in a mouse model. Experimental and Therapeutic Medicine, 9, 1006-1012. https://doi.org/10.3892/etm.2015.2204
MLA
Ma, T., Xu, J., Zhuang, J., Zhou, X., Lin, L., Shan, Z., Qi, Z."Combination of C-X-C motif chemokine 9 and C-X-C motif chemokine 10 antibodies with FTY720 prolongs the survival of cardiac retransplantation allografts in a mouse model". Experimental and Therapeutic Medicine 9.3 (2015): 1006-1012.
Chicago
Ma, T., Xu, J., Zhuang, J., Zhou, X., Lin, L., Shan, Z., Qi, Z."Combination of C-X-C motif chemokine 9 and C-X-C motif chemokine 10 antibodies with FTY720 prolongs the survival of cardiac retransplantation allografts in a mouse model". Experimental and Therapeutic Medicine 9, no. 3 (2015): 1006-1012. https://doi.org/10.3892/etm.2015.2204
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