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microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo

  • Authors:
    • Zhuoqi Zhang
    • Yong Li
    • Liqiao Fan
    • Qun Zhao
    • Bibo Tan
    • Zhong Li
    • Aimin Zang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Department of Oncological Surgery, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1617-1622
    |
    Published online on: February 25, 2015
       https://doi.org/10.3892/etm.2015.2318
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Abstract

Emerging evidence indicates that microRNAs are involved in the development and progression of gastric cancer (GC), functioning as tumor suppressors or oncogenes. The aim of the current study was to explore the potential mechanism of microRNA-425-5p (miR‑425‑5p) in GC. Using reverse transcription quantitative polymerase chain reaction, miR‑425‑5p expression was detected in GC cell lines (SGC‑7901, MKN-28, MKN-45, BGC-823 and AGS) and the GES-1 cell line. Cell proliferation, soft agar colony formation, flow cytometry, haptotactic migration and matrigel chemoinvasion assays were used to test the proliferation, apoptosis, invasion and migration of BGC-823 cells transfected with miR‑425‑5p mimics or an inhibitor. Female BALB/c mice were randomly divided into three groups. Each experimental group contained five mice. BGC 823 cells were stably transfected with miR‑425‑5p inhibitor, wild type or negative control and were injected into the mice through the tail vein. It was found that miR‑425‑5p expression was significantly upregulated in all the GC cell lines when compared with the GES-1 cell line. Downregulation of miR‑425‑5p expression inhibited GC cell proliferation, invasion and migration.In the in vivo studies, downregulation of miR‑425‑5p expression suppressed pulmonary metastasis in the nude mice. Thus, miR‑425‑5p was demonstrated to have the potential to become a novel metastasis‑associated gene; however, the mechanisms associated with these effects require further study. In the future, the development of miR‑425‑5p as a novel therapeutic strategy for the treatment of GC may be possible.
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1 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Kamangar F, Dores GM and Anderson WF: Patterns of cancer incidence, mortality, and prevalence across five continents: defining priorities to reduce cancer disparities in different geographic regions of the world. J Clin Oncol. 24:2137–2150. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Catalano V, Labianca R, Beretta GD, Gatta G, de Braud F and Van Cutsem E: Gastric cancer. Crit Rev Oncol Hematol. 71:127–164. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Parkin DM, Bray F, Ferlay and Pisani P: Global cancer statistics 2002. CA Cancer J Clin. 55:74–108. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Chen CZ: MicroRNAs as oncogenes and tumor suppressors. N Engl J Med. 353:1768–1771. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Friedman RC, Farh KK, Burge CB and Bartel DP: Most mammalian mRNAs are conserved targets of microRNAs. Genome Res. 19:92–105. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Carthew RW and Sontheimer EJ: Origins and mechanisms of miRNAs and siRNAs. Cell. 136:642–655. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Mirnezami AH, Pickard K, Zhang L, Primrose JN and Packham G: MicroRNAs: key players in carcinogenesis and novel therapeutic targets. Eur J Surg Oncol. 35:339–347. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Ruan K, Fang X and Ouyang G: MicroRNAs: novel regulators in the hallmarks of human cancer. Cancer Lett. 285:116–126. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Kim K, Chadalapaka G, Pathi SS, et al: Induction of the transcriptional repressor ZBTB4 in prostate cancer cells by drug-induced targeting of microRNA-17-92/106b-25 clusters. Mol Cancer Ther. 11:1852–1862. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Selcuklu SD, Donoghue MT, Rehmet K, et al: MicroRNA-9 inhibition of cell proliferation and identification of novel miR-9 targets by transcriptome profiling in breast cancer cells. J Biol Chem. 287:29516–29528. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Dey N, Das F, Ghosh-Choudhury N, et al: microRNA-21 governs TORC1 activation in renal cancer cell proliferation and invasion. PLoS One. 7:e373662012. View Article : Google Scholar : PubMed/NCBI

14 

He HC, Zhu JG, Chen XB, et al: MicroRNA-23b downregulates peroxiredoxin III in human prostate cancer. FEBS Lett. 586:2451–2458. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Hur K, Toiyama Y, Takahashi M, et al: MicroRNA-200c modulates epithelial-to-mesenchymal transition (EMT) in human colorectal cancer metastasis. Gut. 62:1315–1326. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Ueda T, Volinia S, Okumura H, et al: Relation between microRNA expression and progression and prognosis of gastric cancer: a microRNA expression analysis. Lancet Oncol. 11:136–146. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Di Leva G, Piovan C, Gasparini P, et al: Estrogen mediated-activation of miR-191/425 cluster modulates tumorigenicity of breast cancer cells depending on estrogen receptor status. PLoS Genet. 9:e10033112013. View Article : Google Scholar : PubMed/NCBI

18 

Peng WZ, Ma R, Wang F, Yu J and Liu ZB: Role of miR-191/425 cluster in tumorigenesis and diagnosis of gastric cancer. Int J Mol Sci. 15:4031–4048. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Sander S, Bullinger L, Klapproth K, Fiedler K, et al: MYC stimulates EZH2 expression by repression of its negative regulator miR-26a. Blood. 112:4202–4212. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Jazbutyte V and Thum T: MicroRNA-21: from cancer to cardiovascular disease. Current Drug Targets. 11:926–935. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Uziel T, Karginov FV, Xie S, Parker JS, et al: The miR-17∼92 cluster collaborates with the Sonic Hedgehog pathway in medulloblastoma. Proc Natl Acad Sci USA. 106:2812–2817. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Bryant RJ, Pawlowski T, Catto JW, Marsden G, et al: Changes in circulating microRNA levels associated with prostate cancer. Br J Cancer. 106:768–774. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Wang M, Gu H, Qian H, Zhu W, et al: miR-17-5p/20a are important markers for gastric cancer and murine double minute 2 participates in their functional regulation. Eur J Cancer. 49:2010–2021. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Mavrakis KJ, Wolfe AL, Oricchio E, Palomero T, et al: Genome-wide RNA-mediated interference screen identifies miR-19 targets in Notch-induced T-cell acute lymphoblastic leukaemia. Nat Cell Biol. 12:372–379. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Hsu KW, Wang AM, Ping YH, Huang KH, et al: Downregulation of tumor suppressor MBP-1 by microRNA-363 in gastric carcinogenesis. Carcinogenesis. 35:208–217. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Ma J, Liu J, Wang Z, et al: NF-kappaB-dependent microRNA-425 upregulation promotes gastric cancer cell growth by targeting PTEN upon IL-1beta induction. Mol Cancer. 13:402014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang Z, Li Y, Fan L, Zhao Q, Tan B, Li Z and Zang A: microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo. Exp Ther Med 9: 1617-1622, 2015.
APA
Zhang, Z., Li, Y., Fan, L., Zhao, Q., Tan, B., Li, Z., & Zang, A. (2015). microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo. Experimental and Therapeutic Medicine, 9, 1617-1622. https://doi.org/10.3892/etm.2015.2318
MLA
Zhang, Z., Li, Y., Fan, L., Zhao, Q., Tan, B., Li, Z., Zang, A."microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo". Experimental and Therapeutic Medicine 9.5 (2015): 1617-1622.
Chicago
Zhang, Z., Li, Y., Fan, L., Zhao, Q., Tan, B., Li, Z., Zang, A."microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo". Experimental and Therapeutic Medicine 9, no. 5 (2015): 1617-1622. https://doi.org/10.3892/etm.2015.2318
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Z, Li Y, Fan L, Zhao Q, Tan B, Li Z and Zang A: microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo. Exp Ther Med 9: 1617-1622, 2015.
APA
Zhang, Z., Li, Y., Fan, L., Zhao, Q., Tan, B., Li, Z., & Zang, A. (2015). microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo. Experimental and Therapeutic Medicine, 9, 1617-1622. https://doi.org/10.3892/etm.2015.2318
MLA
Zhang, Z., Li, Y., Fan, L., Zhao, Q., Tan, B., Li, Z., Zang, A."microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo". Experimental and Therapeutic Medicine 9.5 (2015): 1617-1622.
Chicago
Zhang, Z., Li, Y., Fan, L., Zhao, Q., Tan, B., Li, Z., Zang, A."microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo". Experimental and Therapeutic Medicine 9, no. 5 (2015): 1617-1622. https://doi.org/10.3892/etm.2015.2318
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