B7 homolog 3 aggravates brain injury in a murine model of Streptococcus pneumoniae‑induced meningitis

  • Authors:
    • Xuqin Chen
    • Yanping Wang
    • Zhedong Wang
    • Ruhong Yan
    • Jie Liu
    • Xiangying Meng
    • Yan Li
    • Jianghuai Wang
    • Jian Wang
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  • Published online on: March 5, 2015     https://doi.org/10.3892/etm.2015.2333
  • Pages: 1984-1990
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Abstract

Despite the application of antibiotics, Streptococcus pneumoniae (SP)‑induced meningitis continues to be a life-threatening disease with a high fatality rate and an elevated risk of serious neurological sequelae, particularly in developing countries. In this study, the contribution of the co-stimulatory molecule B7 homolog 3 (B7-H3) to the pathogenesis of experimental SP‑induced meningitis was investigated. Mice were challenged with the intracerebroventricular injection of serotype 3 SP with or without B7-H3. The clinical status of mice with SP‑induced meningitis was examined by body weight loss and spontaneous motor activity with neurological scoring. Coronal brain sections were analyzed by counting Nissl-positive neurons and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL)-positive cells. Protein expression of neuron‑specific enolase (NSE) and S100B in brain tissues was examined with immunohistochemical staining. All experiments were performed in a randomized and blinded setting. By the intracerebroventricular injection of SP suspension, a murine model of pneumococcal meningitis was successfully established. In this SP-induced meningitis model, B7-H3 deteriorated the clinical status, as manifested by a decreased neurological score and increased body weight loss. Following the B7-H3 challenge, the number of Nissl-positive cells decreased and TUNEL‑stained positive cells increased in the brain tissues of mice with SP meningitis, which demonstrates the enhancement of neuronal necrosis and apoptosis, respectively. Protein expression of NSE was decreased, while that of S100B was increased. These in vivo findings indicate that B7-H3 aggravates brain injury during the pathological process of experimental SP-induced meningitis.
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May-2015
Volume 9 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen X, Wang Y, Wang Z, Yan R, Liu J, Meng X, Li Y, Wang J and Wang J: B7 homolog 3 aggravates brain injury in a murine model of Streptococcus pneumoniae‑induced meningitis. Exp Ther Med 9: 1984-1990, 2015
APA
Chen, X., Wang, Y., Wang, Z., Yan, R., Liu, J., Meng, X. ... Wang, J. (2015). B7 homolog 3 aggravates brain injury in a murine model of Streptococcus pneumoniae‑induced meningitis. Experimental and Therapeutic Medicine, 9, 1984-1990. https://doi.org/10.3892/etm.2015.2333
MLA
Chen, X., Wang, Y., Wang, Z., Yan, R., Liu, J., Meng, X., Li, Y., Wang, J., Wang, J."B7 homolog 3 aggravates brain injury in a murine model of Streptococcus pneumoniae‑induced meningitis". Experimental and Therapeutic Medicine 9.5 (2015): 1984-1990.
Chicago
Chen, X., Wang, Y., Wang, Z., Yan, R., Liu, J., Meng, X., Li, Y., Wang, J., Wang, J."B7 homolog 3 aggravates brain injury in a murine model of Streptococcus pneumoniae‑induced meningitis". Experimental and Therapeutic Medicine 9, no. 5 (2015): 1984-1990. https://doi.org/10.3892/etm.2015.2333