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Article

Cloning, expression and identification of an isoform of human stromal cell derived factor-1α

  • Authors:
    • Yin‑Ku Liang
    • Wei Ping
    • Liu‑Jiao Bian
  • View Affiliations / Copyright

    Affiliations: College of Life Science, Northwest University, Xi'an, Shaanxi 710069, P.R. China
  • Pages: 1751-1756
    |
    Published online on: March 16, 2015
       https://doi.org/10.3892/etm.2015.2355
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Abstract

Human stromal cell derived factor‑1α (hSDF‑1α), a chemotactic factor of stem cells, regulates inflammation, promotes the mobilization of stem cells and induces angiogenesis following ischemia. Six SDF‑1 isoforms, SDF‑1α, SDF‑1β, SDF‑1γ, SDF‑1δ, SDF‑1ε and SDF‑1ϕ, which all contain a signal peptide at the N‑terminus, have been reported. In the present study a special isoform of hSDF‑1α is described that does not contain the N‑terminal signal peptide sequence. The hSDF‑1α gene was cloned with the recombinant plasmid pCMV‑SPORT6‑hSDF1 as the template, and the prokaryotic expression vector pET15b‑hSDF‑1α was constructed. This hSDF‑1α was successfully expressed as an inclusion body in Escherichia coli BL21(DE3). The recombinant hSDF‑1α was refolded in vitro and separated by cation exchange chromatography. Following these two steps the purity of the hSDF‑1α was able to reach >85%. The recombinant hSDF‑1α was then purified by size‑exclusion chromatography. SDS‑PAGE analysis demonstrated that the purity of the hSDF‑1α was >95%, which meets almost all the requirements of a protein experiment. Chemotactic activity of the recombinant hSDF‑1α was analyzed by Transwell migration assay and it was found that the recombinant hSDF‑1α was able to stimulate THP‑1 cell migration. These data suggest that the procedure of producing recombinant hSDF‑1α proteins with chemotactic activity was feasible and the N‑terminal signal peptide of hSDF‑1α has little effect on the chemotactic activity of hSDF‑1α.
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1 

Voermans C, Anthony EC, Mul E, van der Schoot E and Hordijk P: SDF-1-induced actin polymerization and migration in human hematopoietic progenitor cells. Exp Hematol. 29:1456–1464. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Shi HW, Zhang P, Wang CM and Han H: Prokaryotic expression and purification of fusion protein GST-SDF-1α. Ke Xue Ji Shu Yu Gong Cheng. 7:3365–3367. 2007.[(In Chinese)].

3 

Yao F, Zhou JM, Wang Z, Wei DH, Jiang ZS, Liu LS and Tong ZY: Cloning and sequence analysis of SDF-1α gene in rats. Zhongguo Shi Yan Dong Wu Xue Bao. 16:14–18. 2008.[(In Chinese)].

4 

Nagasawa T, Kikutani H and Kishimoto T: Molecular cloning and structure of a pre-B-cell growth-stimulating factor. Proc Natl Acad Sci USA. 91:2305–2309. 1994. View Article : Google Scholar : PubMed/NCBI

5 

Psenák O: Stromal cell-derived factor-1 (SDF-1). Its structure and function. Cas Lek Cesk. 140:355–363. 2001.[(In Czech)]. PubMed/NCBI

6 

Shirozu M, Nakano T, Inazawa J, Tashiro K, et al: Structure and chromosomal localization of the human stromal cell derived factor-1 (SDF-1) gene. Genomics. 28:495–500. 1995. View Article : Google Scholar : PubMed/NCBI

7 

Gleichmann M, Gillen C, Czardybon M, Bosse F, et al: Cloning and characterization of SDF-1γ, a novel SDF-1 chemokine transcript with developmentally regulated expression in the nervous system. Eur J Neurosci. 12:1857–1866. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Yu L, Cecil J, Peng SB, Schrementi J, Kovacevic S, Paul D, Su EW and Wang J: Identification and expression of novel isoforms of human stromal cell-derived factor 1. Gene. 374:174–179. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Feng Y: Cloning of SDF-1α gene and effect on migration of THP-1 (PhD thesis)Nanhua University; 2006

10 

Crump MP, Gong JH, Loetscher P, Rajarathnam K, Amara A, Arenzana-Seisdedos F, Virelizier JL, et al: Solution structure and basis for functional activity of stromal cell-derived factor-1; dissociation of CXCR4 activation from binding and inhibition of HIV-1. EMBO J. 16:6996–7007. 1997. View Article : Google Scholar : PubMed/NCBI

11 

Yu M, Liang Q, Lu HL, Mao WW, Wu MY, Wang Q and Han W: Expression and purification of human SDF-1α in prokarotic cells and its regulative role in murine bone marrow hematopoiesis. Xian Dai Sheng Wu Yi Xue Jin Zhan. 7:836–839. 2007.[(In Chinese)].

12 

Cao ZW: An effective method of Tricine-SDS-PAGE for separating the 1kDa peptide. Zhong Guo Sheng Wu Gong Cheng Za Zhi. 38:74–76. 2003.[(In Chinese)].

13 

Miao L, Xu LH, Ji X and Bian LJ: Cloning, expression, purification and its biological activity study of the soluble tripolymer recombinant angiogenesis inhibitor kringle 5. Sheng Wu Gong Cheng Jin Zhan. 31:18–22. 2011.[(In Chinese)].

14 

Kodali RB, Kim WJ, Galaria II, Miller C, Schecter AD, Lira SA and Taubman MB: CCL11 (Eotaxin) induces CCR3-dependent smooth muscle cell migration. Arterioscler Thromb Vasc Biol. 24:1211–1216. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Lv YC, Wang Z, Wei DH, et al: The chemotactic activity of stromal cell derived factor 1α-CXCR4 to the migration of THP-1 cell and enhanced effect of oxidized low density lipoprotein. Zhongguo Dong Mai Ying Hua Za Zhi. 15:15–18. 2007.[(In Chinese)].

16 

Wu B, Chien EY, Mol CD, Fenalti G, Liu W, Katritch V, Abagyan R, Brooun A, Wells P, Bi FC, et al: Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists. Science. 330:1066–1071. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Penn MS: Importance of the SDF-1:CXCR4 axis in myocardial repair. Circ Res. 104:1133–1135. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Jujo K, Hamada H, Iwakura A, Thorne T, Sekiguchi H, Clarke T, Ito A, Misener S, Tanaka T, Klyachko E, et al: CXCR4 blockade augments bone marrow progenitor cell recruitment to the neovasculature and reduces mortality after myocardial infarction. Proc Natl Acad Sci USA. 107:11008–11013. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Li YJ, Cai XX, Ma GS, Chen ZH, Tang CC and Feng Y: The short-term prognostic value of stromal cell-derived factor-1α in patients with acute myocardial infarction. Nanjing Gongye Daxue Xxuebao: Ziran Kexue Ban. 31:1781–1784. 2011.[(In Chinese)].

20 

Stumm RK, Rummel J, Junker V, Culmsee C, Pfeiffer M, Krieglstein J, Höllt V and Schulz S: A dual role for the SDF-1/CXCR4 chemokine receptor system in adult brain: isoform-selective regulation of SDF-1 expression modulates CXCR4-dependent neuronal plasticity and cerebral leukocyte recruitment after focal ischemia. J Neurosci. 22:5865–5878. 2002.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liang YK, Ping W and Bian LJ: Cloning, expression and identification of an isoform of human stromal cell derived factor-1α. Exp Ther Med 9: 1751-1756, 2015.
APA
Liang, Y., Ping, W., & Bian, L. (2015). Cloning, expression and identification of an isoform of human stromal cell derived factor-1α. Experimental and Therapeutic Medicine, 9, 1751-1756. https://doi.org/10.3892/etm.2015.2355
MLA
Liang, Y., Ping, W., Bian, L."Cloning, expression and identification of an isoform of human stromal cell derived factor-1α". Experimental and Therapeutic Medicine 9.5 (2015): 1751-1756.
Chicago
Liang, Y., Ping, W., Bian, L."Cloning, expression and identification of an isoform of human stromal cell derived factor-1α". Experimental and Therapeutic Medicine 9, no. 5 (2015): 1751-1756. https://doi.org/10.3892/etm.2015.2355
Copy and paste a formatted citation
x
Spandidos Publications style
Liang YK, Ping W and Bian LJ: Cloning, expression and identification of an isoform of human stromal cell derived factor-1α. Exp Ther Med 9: 1751-1756, 2015.
APA
Liang, Y., Ping, W., & Bian, L. (2015). Cloning, expression and identification of an isoform of human stromal cell derived factor-1α. Experimental and Therapeutic Medicine, 9, 1751-1756. https://doi.org/10.3892/etm.2015.2355
MLA
Liang, Y., Ping, W., Bian, L."Cloning, expression and identification of an isoform of human stromal cell derived factor-1α". Experimental and Therapeutic Medicine 9.5 (2015): 1751-1756.
Chicago
Liang, Y., Ping, W., Bian, L."Cloning, expression and identification of an isoform of human stromal cell derived factor-1α". Experimental and Therapeutic Medicine 9, no. 5 (2015): 1751-1756. https://doi.org/10.3892/etm.2015.2355
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