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Neuroprotective effect of interleukin-6 in a rat model of cerebral ischemia

  • Authors:
    • Qilin Feng
    • Yi Wang
    • Yingda Yang
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Central Hospital of Wuhan, Wuhan, Hubei 430030, P.R. China, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1695-1701
    |
    Published online on: March 16, 2015
       https://doi.org/10.3892/etm.2015.2363
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Abstract

Interleukin (IL)-6 is known to be a key cytokine in immune regulation in addition to serving crucial functions in various autoimmune diseases; however, the neuroprotective potential of IL‑6 has not been fully investigated. The aim of the present study was to investigate the neuroprotective effects of the inflammatory cytokine IL‑6 in a rat model of cerebral ischemia. Rat cerebral ischemia was induced by intraluminal middle cerebral artery occlusion. Following treatment with 500 or 50 ng IL‑6, the infarct volumes and symptoms of neurological deficit were ameliorated. Furthermore, terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling staining suggested that the IL‑6 treatment reduced neuronal apoptosis in vivo, which was consistent with a lower percentage of annexin V- and caspase‑3‑positive cortical neurons. In addition, IL‑6 in vitro induced the phosphorylation of signal transducer and activator of transcription (STAT) 3 and the expression of induced myeloid leukemia cell differentiation protein Mcl‑1, but not the expression of B‑cell lymphoma 2, suggesting the activation of the Janus kinase/STAT pathway by IL‑6. IL‑6 also appeared to be involved in the regulation of cytokine secretion and blood‑brain barrier (BBB) integrity in cerebral ischemia. IL‑6 downregulated a number of inflammatory cytokines, including tumor necrosis factor‑α and IL‑1β, as well as myeloperoxidase activity, indicating the accumulation of granulocytes in the ischemic brain tissue. IL‑6 was also observed to support the integrity of the BBB by reducing Evans blue leakage in vivo and suppressing the expression of matrix metalloproteinase‑9 in ischemic brain tissue. In conclusion, the results of the present study indicate that the neuroprotective effects of IL‑6 in cerebral ischemia are the result of a range of processes, including the modulation of cell apoptosis, cytokine secretion and the integrity of the BBB. IL‑6 could therefore be used as a therapeutic agent in clinical practice.
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Copy and paste a formatted citation
Spandidos Publications style
Feng Q, Wang Y and Yang Y: Neuroprotective effect of interleukin-6 in a rat model of cerebral ischemia. Exp Ther Med 9: 1695-1701, 2015.
APA
Feng, Q., Wang, Y., & Yang, Y. (2015). Neuroprotective effect of interleukin-6 in a rat model of cerebral ischemia. Experimental and Therapeutic Medicine, 9, 1695-1701. https://doi.org/10.3892/etm.2015.2363
MLA
Feng, Q., Wang, Y., Yang, Y."Neuroprotective effect of interleukin-6 in a rat model of cerebral ischemia". Experimental and Therapeutic Medicine 9.5 (2015): 1695-1701.
Chicago
Feng, Q., Wang, Y., Yang, Y."Neuroprotective effect of interleukin-6 in a rat model of cerebral ischemia". Experimental and Therapeutic Medicine 9, no. 5 (2015): 1695-1701. https://doi.org/10.3892/etm.2015.2363
Copy and paste a formatted citation
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Spandidos Publications style
Feng Q, Wang Y and Yang Y: Neuroprotective effect of interleukin-6 in a rat model of cerebral ischemia. Exp Ther Med 9: 1695-1701, 2015.
APA
Feng, Q., Wang, Y., & Yang, Y. (2015). Neuroprotective effect of interleukin-6 in a rat model of cerebral ischemia. Experimental and Therapeutic Medicine, 9, 1695-1701. https://doi.org/10.3892/etm.2015.2363
MLA
Feng, Q., Wang, Y., Yang, Y."Neuroprotective effect of interleukin-6 in a rat model of cerebral ischemia". Experimental and Therapeutic Medicine 9.5 (2015): 1695-1701.
Chicago
Feng, Q., Wang, Y., Yang, Y."Neuroprotective effect of interleukin-6 in a rat model of cerebral ischemia". Experimental and Therapeutic Medicine 9, no. 5 (2015): 1695-1701. https://doi.org/10.3892/etm.2015.2363
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