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Article Open Access

Association between follicular fluid levels of HMGB1 protein and outcomes in patients undergoing in vitro fertilization/intracytoplasmic sperm injection cycles

  • Authors:
    • Saijiao Li
    • Tailang Yin
    • Wei Li
    • Jing Yang
    • Wangming Xu
    • Danni Zhou
  • View Affiliations / Copyright

    Affiliations: Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1611-1616
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    Published online on: March 18, 2015
       https://doi.org/10.3892/etm.2015.2369
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Abstract

The aim of the present study was to evaluate the association between follicular fluid (FF) levels of high‑mobility group box 1 (HMGB1) protein and the reproductive outcome in patients undergoing in vitro fertilization (IVF) with intracytoplasmic sperm injection (ICSI). FF samples were collected from the ovarian follicles (≥14 mm) of 143 infertile patients that had undergone IVF/ICSI, and the HMGB1 expression levels were determined using ELISA. Spearman's correlation and receiver operating characteristic (ROC) curve analysis were applied to analyze the results. Significantly increased levels of HMGB1 protein (7.38±2.02 vs. 6.14±2.52 ng/ml; P<0.01), endometrial thickness on the day of human chorionic gonadotropin (hCG) administration (10.3±1.3 vs. 9.7±1.7 mm; P<0.01) and retrieved oocyte counts (11.68±6.51 vs. 11.00±6.34; P<0.01) were observed in the pregnant group when compared with the non‑pregnant group. Conversely, the level of luteinizing hormone on the day of hCG administration was significantly reduced in the pregnant group compared with the non‑pregnant group (0.92±1.78 vs. 1.78±2.03 pmol/l, P<0.01). The ROC curve indicated a significant association between the FF level of HMGB1 protein and the pregnancy rate, with an area under the ROC curve of 0.673 (0.581‑0.765; P<0.01). In addition, the HMGB1 protein level was shown to have a significant positive correlation with the endometrial thickness (r=0.170; P<0.05). Therefore, the present study indicated that the FF levels of HMGB1 protein are increased in pregnant patients and are positively correlated with endometrial thickness. Thus, FF levels of HMGB1 may be a useful factor for predicting the outcome of IVF/ICSI treatments.
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Copy and paste a formatted citation
Spandidos Publications style
Li S, Yin T, Li W, Yang J, Xu W and Zhou D: Association between follicular fluid levels of HMGB1 protein and outcomes in patients undergoing in vitro fertilization/intracytoplasmic sperm injection cycles. Exp Ther Med 9: 1611-1616, 2015.
APA
Li, S., Yin, T., Li, W., Yang, J., Xu, W., & Zhou, D. (2015). Association between follicular fluid levels of HMGB1 protein and outcomes in patients undergoing in vitro fertilization/intracytoplasmic sperm injection cycles. Experimental and Therapeutic Medicine, 9, 1611-1616. https://doi.org/10.3892/etm.2015.2369
MLA
Li, S., Yin, T., Li, W., Yang, J., Xu, W., Zhou, D."Association between follicular fluid levels of HMGB1 protein and outcomes in patients undergoing in vitro fertilization/intracytoplasmic sperm injection cycles". Experimental and Therapeutic Medicine 9.5 (2015): 1611-1616.
Chicago
Li, S., Yin, T., Li, W., Yang, J., Xu, W., Zhou, D."Association between follicular fluid levels of HMGB1 protein and outcomes in patients undergoing in vitro fertilization/intracytoplasmic sperm injection cycles". Experimental and Therapeutic Medicine 9, no. 5 (2015): 1611-1616. https://doi.org/10.3892/etm.2015.2369
Copy and paste a formatted citation
x
Spandidos Publications style
Li S, Yin T, Li W, Yang J, Xu W and Zhou D: Association between follicular fluid levels of HMGB1 protein and outcomes in patients undergoing in vitro fertilization/intracytoplasmic sperm injection cycles. Exp Ther Med 9: 1611-1616, 2015.
APA
Li, S., Yin, T., Li, W., Yang, J., Xu, W., & Zhou, D. (2015). Association between follicular fluid levels of HMGB1 protein and outcomes in patients undergoing in vitro fertilization/intracytoplasmic sperm injection cycles. Experimental and Therapeutic Medicine, 9, 1611-1616. https://doi.org/10.3892/etm.2015.2369
MLA
Li, S., Yin, T., Li, W., Yang, J., Xu, W., Zhou, D."Association between follicular fluid levels of HMGB1 protein and outcomes in patients undergoing in vitro fertilization/intracytoplasmic sperm injection cycles". Experimental and Therapeutic Medicine 9.5 (2015): 1611-1616.
Chicago
Li, S., Yin, T., Li, W., Yang, J., Xu, W., Zhou, D."Association between follicular fluid levels of HMGB1 protein and outcomes in patients undergoing in vitro fertilization/intracytoplasmic sperm injection cycles". Experimental and Therapeutic Medicine 9, no. 5 (2015): 1611-1616. https://doi.org/10.3892/etm.2015.2369
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