Expression of WTH3 in breast cancer tissue and the effects on the biological behavior of breast cancer cells

Gan,Lin; Zuo,Guoqing; Wang,Ting; Min,Jie; Wang,Yadong; Wang,Yongyue; Lv,Gang

doi:10.3892/etm.2015.2458

Expression of WTH3 in breast cancer tissue and the effects on the biological behavior of breast cancer cells

Authors:
- Lin Gan
- Guoqing Zuo
- Ting Wang
- Jie Min
- Yadong Wang
- Yongyue Wang
- Gang Lv
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Affiliations: Department of Mammary Gland and Thyroid Gland, Chongqing Hospital of Traditional Chinese Medicine, Chongqing 404100, P.R. China, Department of Gastroenterology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing 404100, P.R. China
Published online on: April 29, 2015 https://doi.org/10.3892/etm.2015.2458
Pages: 154-158

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Abstract

The aim of the present study was to investigate the expression of WTH3 in tumor and normal breast tissue. The mRNA and protein expression levels of WTH3 were detected using reverse transcription quantitative polymerase chain reaction and western blot analysis, respectively. In addition, matrix metalloproteinase (MMP)‑2 protein expression was measured. The effect of WTH3 expression on the proliferation activity of breast cancer cells was detected using a Cell Counting Kit‑8 assay. Furthermore, the effects of WTH3 on the invasion and migration ability of the breast cancer cells was investigated. The results revealed that WTH3 was able to significantly inhibit the proliferation of the MCF‑7 and MDA‑MB‑231 breast cancer cell lines. In addition, the invasion and migration assay demonstrated that WTH3 was able to inhibit the invasion and migration of breast cancer cells. Western blot analysis revealed that increased expression of WTH3 resulted in decreased expression levels of MMP‑2, which has an important function in the metastasis of cancer cells. In conclusion, WTH3 expression differed between the tumor and normal breast tissues. WTH3 was able to inhibit the proliferation of breast cancer cells and decrease their invasion ability. Thus, WTH3 may be a promising target for breast cancer therapy in the future.

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