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Protective effects of paeoniflorin against FasL‑induced apoptosis of intervertebral disc annulus fibrosus cells via Fas‑FasL signalling pathway

  • Authors:
    • Shao‑Qing Chen
    • Jian‑Ping Lin
    • Qi‑Kai Zheng
    • Shui‑Jin Chen
    • Ming Li
    • Xian‑Zhao Lin
    • Shi‑Zhong Wang

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  • Published online on: September 25, 2015     https://doi.org/10.3892/etm.2015.2776
  • Pages: 2351-2355
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Abstract

In the present study, we demonstrate that the degeneration of intervertebral discs is caused by ageing and apoptosis of matrix cells. Apoptosis is as essential as the function of proteoglycan synthesis in assessing the possible degeneration of intervertebral discs; paeoniflorin (PF) induces cytoprotective effects on various types of cells. In this study, the function of PF in inhibiting Fas ligand (FasL)‑induced apoptosis in annulus fibrosus cells was assessed, and the correlation between apoptosis and the Fas‑FasL pathway was determined. Annulus fibrosus cells were derived from the intervertebral discs of 1‑month‑old Sprague Dawley rats; the cells were characterised by toluidine blue staining and subjected to apoptosis with FasL. PF was diluted to various concentrations and added to annulus fibrosus cells at various times. The impact of PF and FasL on cell apoptosis of annulus fibrosus cells was determined by flow cytometry. Western blot analysis was performed to determine the protein expression levels of Fas and caspase‑3. The percentages of apoptotic annulus fibrosus cells as well as the expression levels of caspase‑3 and Fas were significantly reduced following treatment with 208, 20.8 or 2.08 µM PF. PF inhibits the activation of the Fas‑FasL signal pathway and decreases FasL‑induced apoptosis of annulus fibrosus cells.
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December-2015
Volume 10 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen SQ, Lin JP, Zheng QK, Chen SJ, Li M, Lin XZ and Wang SZ: Protective effects of paeoniflorin against FasL‑induced apoptosis of intervertebral disc annulus fibrosus cells via Fas‑FasL signalling pathway. Exp Ther Med 10: 2351-2355, 2015
APA
Chen, S., Lin, J., Zheng, Q., Chen, S., Li, M., Lin, X., & Wang, S. (2015). Protective effects of paeoniflorin against FasL‑induced apoptosis of intervertebral disc annulus fibrosus cells via Fas‑FasL signalling pathway. Experimental and Therapeutic Medicine, 10, 2351-2355. https://doi.org/10.3892/etm.2015.2776
MLA
Chen, S., Lin, J., Zheng, Q., Chen, S., Li, M., Lin, X., Wang, S."Protective effects of paeoniflorin against FasL‑induced apoptosis of intervertebral disc annulus fibrosus cells via Fas‑FasL signalling pathway". Experimental and Therapeutic Medicine 10.6 (2015): 2351-2355.
Chicago
Chen, S., Lin, J., Zheng, Q., Chen, S., Li, M., Lin, X., Wang, S."Protective effects of paeoniflorin against FasL‑induced apoptosis of intervertebral disc annulus fibrosus cells via Fas‑FasL signalling pathway". Experimental and Therapeutic Medicine 10, no. 6 (2015): 2351-2355. https://doi.org/10.3892/etm.2015.2776