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Article

Protective effects of paeoniflorin against FasL‑induced apoptosis of intervertebral disc annulus fibrosus cells via Fas‑FasL signalling pathway

  • Authors:
    • Shao‑Qing Chen
    • Jian‑Ping Lin
    • Qi‑Kai Zheng
    • Shui‑Jin Chen
    • Ming Li
    • Xian‑Zhao Lin
    • Shi‑Zhong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, The Affiliated Rehabilitation Hospital to Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350000, P.R. China, Ministry of Education (Provincial Total Construction) Key Laboratory of Chinese Orthopedics and Athletic Rehabilitation, Fuzhou, Fujian 350000, P.R. China
  • Pages: 2351-2355
    |
    Published online on: September 25, 2015
       https://doi.org/10.3892/etm.2015.2776
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Abstract

In the present study, we demonstrate that the degeneration of intervertebral discs is caused by ageing and apoptosis of matrix cells. Apoptosis is as essential as the function of proteoglycan synthesis in assessing the possible degeneration of intervertebral discs; paeoniflorin (PF) induces cytoprotective effects on various types of cells. In this study, the function of PF in inhibiting Fas ligand (FasL)‑induced apoptosis in annulus fibrosus cells was assessed, and the correlation between apoptosis and the Fas‑FasL pathway was determined. Annulus fibrosus cells were derived from the intervertebral discs of 1‑month‑old Sprague Dawley rats; the cells were characterised by toluidine blue staining and subjected to apoptosis with FasL. PF was diluted to various concentrations and added to annulus fibrosus cells at various times. The impact of PF and FasL on cell apoptosis of annulus fibrosus cells was determined by flow cytometry. Western blot analysis was performed to determine the protein expression levels of Fas and caspase‑3. The percentages of apoptotic annulus fibrosus cells as well as the expression levels of caspase‑3 and Fas were significantly reduced following treatment with 208, 20.8 or 2.08 µM PF. PF inhibits the activation of the Fas‑FasL signal pathway and decreases FasL‑induced apoptosis of annulus fibrosus cells.
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1 

Ahmed SH, El-Shaarawy EA, Ishaq MF and Moniem MH: Morphological and radiometrical study of the human intervertebral foramina of the cervical spine. Folia Morphol (Warsz). 73:7–18. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Kong D, Zheng T, Fang J and Li X: Apoptosis of endplate chondrocytes in post-laminectomy cervical kyphotic deformity. An in vivo animal model in sheep. Eur Spine J. 22:1576–1582. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Jiang L, Zhang X, Zheng X, et al: Apoptosis, senescence, and autophagy in rat nucleus pulposus cells: Implications for diabetic intervertebral disc degeneration. J Orthop Res. 31:692–702. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Chan SC, Ferguson SJ and Gantenbein-Ritter B: The effects of dynamic loading on the intervertebral disc. Eur Spine J. 20:1796–1812. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Ma KG, Shao ZW, Yang SH, et al: Autophagy is activated in compression-induced cell degeneration and is mediated by reactive oxygen species in nucleus pulposus cells exposed to compression. Osteoarthritis Cartilage. 21:2030–2038. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Guan YJ, Zhang Z, Yu C, Ma L, Hu W and Xu L: Phospho-SXXE/D motif mediated TNF receptor 1-TRADD death domain complex formation for T cell activation and migration. J Immunol. 187:1289–1297. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Lee EW, Seo J, Jeong M, Lee S and Song J: The roles of FADD in extrinsic apoptosis and necroptosis. BMB Rep. 45:496–508. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Wang S, Xia P, Shi L and Fan Z: FADD cleavage by NK cell granzyme M enhances its self-association to facilitate procaspase-8 recruitment for auto-processing leading to caspase cascade. Cell Death Differ. 19:605–615. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Sun Z, Wan ZY, Guo YS, Wang HQ and Luo ZJ: FasL on human nucleus pulposus cells prevents angiogenesis in the disc by inducing Fas-mediated apoptosis of vascular endothelial cells. Int J Clin Exp Pathol. 6:2376–2385. 2013.PubMed/NCBI

10 

Wang C, Yuan J, Wu HX, et al: Paeoniflorin inhibits inflammatory responses in mice with allergic contact dermatitis by regulating the balance between inflammatory and anti-inflammatory cytokines. Inflamm Res. 62:1035–1044. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Liu HQ, Zhang WY, Luo XT, Ye Y and Zhu XZ: Paeoniflorin attenuates neuroinflammation and dopaminergic neurodegeneration in the MPTP model of Parkinson's disease by activation of adenosine A1 receptor. Br J Pharmacol. 148:314–325. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Wang D, Wong HK, Feng YB and Zhang ZJ: Paeoniflorin, a natural neuroprotective agent, modulates multiple anti-apoptotic and pro-apoptotic pathways in differentiated PC12 cells. Cell Mol Neurobiol. 33:521–529. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Salunga TL, Tabuchi Y, Takasaki I, et al: Identification of genes responsive to paeoniflorin, a heat shock protein-inducing compound, in human leukemia U937 cells. Int J Hyperthermia. 23:529–537. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Wu D, Chen J, Zhu H, et al: UPLC-PDA determination of paeoniflorin in rat plasma following the oral administration of Radix Paeoniae Alba and its effects on rats with collagen-induced arthritis. Exp Ther Med. 7:209–217. 2014.PubMed/NCBI

15 

Wu YM, Jin R, Yang L, et al: Phosphatidylinositol 3 kinase/protein kinase B is responsible for the protection of paeoniflorin upon H2O2-induced neural progenitor cell injury. Neuroscience. 14:54–62. 2013. View Article : Google Scholar : PubMed/NCBI

16 

The Ministry of Science and Technology of the People's Republic of China: Guidance Suggestions for the Care and Use of Laboratory Animals. 2006.

17 

Liu ZH, Sun Z, Wang HQ, et al: FasL expression on human nucleus pulposus cells contributes to the immune privilege of intervertebral disc by interacting with immunocytes. Int J Med Sci. 10:1053–1060. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Ji Y, Wang T, Wei ZF, Lu GX, Jiang SD, Xia YF and Dai Y: Paeoniflorin, the main active constituent of Paeonia lactiflora roots, attenuates bleomycin-induced pulmonary fibrosis in mice by suppressing the synthesis of type I collagen. J Ethnopharmacol. 149:825–832. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Zhang MH, Feng L, Zhu MM, Gu JF, Wu C and Jia XB: Antioxidative and anti-inflammatory activities of paeoniflorin and oxypaeoniflora on AGEs-induced mesangial cell damage. Planta Med. 79:1319–1323. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Wankun X, Wenzhen Y, Min Z, et al: Protective effect of paeoniflorin against oxidative stress in human retinal pigment epithelium in vitro. Mol Vis. 17:3512–3522. 2011.PubMed/NCBI

21 

Lavrik IN and Krammer PH: Regulation of CD95/Fas signaling at the DISC. Cell Death Differ. 19:36–41. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Ashkenazi A and Dixit VM: Death Receptors: Signaling and modulation. Science. 281(5381): 1305–1308. 1998. View Article : Google Scholar : PubMed/NCBI

23 

Park JB, Chang H and Kim KW: Expression of Fas ligand and apoptosis of disc cells in herniated lumbar disc tissue. Spine. 26:618–621. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Lechardeur D, Drzymala L, Sharma M, et al: Determinants of the nuclear localization of the heterodimeric DNA fragmentation factor (ICAD/CAD). J Cell Biol. 150:321–334. 2000. View Article : Google Scholar : PubMed/NCBI

25 

Mehrkens A, Karim MZ, Kim S, Hilario R, Fehlings MG and Erwin WM: Canine notochordal cell-secreted factors protect murine and human nucleus pulposus cells from apoptosis by inhibition of activated caspase-9 and caspase-3/7. Evid Based Spine Care J. 4:154–156. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen SQ, Lin JP, Zheng QK, Chen SJ, Li M, Lin XZ and Wang SZ: Protective effects of paeoniflorin against FasL‑induced apoptosis of intervertebral disc annulus fibrosus cells via Fas‑FasL signalling pathway. Exp Ther Med 10: 2351-2355, 2015.
APA
Chen, S., Lin, J., Zheng, Q., Chen, S., Li, M., Lin, X., & Wang, S. (2015). Protective effects of paeoniflorin against FasL‑induced apoptosis of intervertebral disc annulus fibrosus cells via Fas‑FasL signalling pathway. Experimental and Therapeutic Medicine, 10, 2351-2355. https://doi.org/10.3892/etm.2015.2776
MLA
Chen, S., Lin, J., Zheng, Q., Chen, S., Li, M., Lin, X., Wang, S."Protective effects of paeoniflorin against FasL‑induced apoptosis of intervertebral disc annulus fibrosus cells via Fas‑FasL signalling pathway". Experimental and Therapeutic Medicine 10.6 (2015): 2351-2355.
Chicago
Chen, S., Lin, J., Zheng, Q., Chen, S., Li, M., Lin, X., Wang, S."Protective effects of paeoniflorin against FasL‑induced apoptosis of intervertebral disc annulus fibrosus cells via Fas‑FasL signalling pathway". Experimental and Therapeutic Medicine 10, no. 6 (2015): 2351-2355. https://doi.org/10.3892/etm.2015.2776
Copy and paste a formatted citation
x
Spandidos Publications style
Chen SQ, Lin JP, Zheng QK, Chen SJ, Li M, Lin XZ and Wang SZ: Protective effects of paeoniflorin against FasL‑induced apoptosis of intervertebral disc annulus fibrosus cells via Fas‑FasL signalling pathway. Exp Ther Med 10: 2351-2355, 2015.
APA
Chen, S., Lin, J., Zheng, Q., Chen, S., Li, M., Lin, X., & Wang, S. (2015). Protective effects of paeoniflorin against FasL‑induced apoptosis of intervertebral disc annulus fibrosus cells via Fas‑FasL signalling pathway. Experimental and Therapeutic Medicine, 10, 2351-2355. https://doi.org/10.3892/etm.2015.2776
MLA
Chen, S., Lin, J., Zheng, Q., Chen, S., Li, M., Lin, X., Wang, S."Protective effects of paeoniflorin against FasL‑induced apoptosis of intervertebral disc annulus fibrosus cells via Fas‑FasL signalling pathway". Experimental and Therapeutic Medicine 10.6 (2015): 2351-2355.
Chicago
Chen, S., Lin, J., Zheng, Q., Chen, S., Li, M., Lin, X., Wang, S."Protective effects of paeoniflorin against FasL‑induced apoptosis of intervertebral disc annulus fibrosus cells via Fas‑FasL signalling pathway". Experimental and Therapeutic Medicine 10, no. 6 (2015): 2351-2355. https://doi.org/10.3892/etm.2015.2776
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