Open Access

Endogenous carbon monoxide downregulates hepatic cystathionine-γ-lyase in rats with liver cirrhosis

  • Authors:
    • Shi‑Bin Guo
    • Zhi‑Jun Duan
    • Qiu‑Ming Wang
    • Qin Zhou
    • Qing Li
    • Xiao‑Yu Sun
  • View Affiliations

  • Published online on: October 22, 2015     https://doi.org/10.3892/etm.2015.2823
  • Pages: 2039-2046
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the effect of endogenous carbon monoxide (CO) on the hydrogen sulfide/cystathionine-γ-lyase (H2S/CSE) pathway in cirrhotic rat livers. The rats were allocated at random into four groups: Sham, cirrhosis, cobalt protoporphyrin (CoPP) and zinc protoporphyrin IX (ZnPP). The expression of hepatic CSE mRNA was evaluated using a quantitative polymerase chain reaction, while CSE protein expression was determined using immunohistochemical analysis. Hematoxylin and eosin staining was performed for the histological evaluation of liver fibrosis. The levels of H2S, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL) and carboxyhemoglobin (COHb) in the arterial blood were determined, in addition to the portal vein pressure. The mRNA and protein expression levels of hepatic CSE and the serum levels of H2S were significantly decreased in the cirrhosis group compared with those in the sham group (P<0.05). Compared with the cirrhosis group, rats in the ZnPP group had significantly lower levels of serum ALT, AST and TBIL, arterial COHb and hepatic fibrosis, while hepatic CSE expression and the production of H2S were significantly increased (P<0.05). The CoPP group exhibited decreased hepatic CSE expression and H2S production, but aggravated hepatic function and fibrosis (P<0.05). In conclusion, the H2S/CSE pathway is involved in the formation of liver cirrhosis and serves a crucial function in protecting liver cells against the progression of liver fibrosis. Endogenous CO downregulates hepatic CSE mRNA and protein expression and the production of H2S in rats with liver cirrhosis.
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December-2015
Volume 10 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Guo SB, Duan ZJ, Wang QM, Zhou Q, Li Q and Sun XY: Endogenous carbon monoxide downregulates hepatic cystathionine-γ-lyase in rats with liver cirrhosis. Exp Ther Med 10: 2039-2046, 2015
APA
Guo, S., Duan, Z., Wang, Q., Zhou, Q., Li, Q., & Sun, X. (2015). Endogenous carbon monoxide downregulates hepatic cystathionine-γ-lyase in rats with liver cirrhosis. Experimental and Therapeutic Medicine, 10, 2039-2046. https://doi.org/10.3892/etm.2015.2823
MLA
Guo, S., Duan, Z., Wang, Q., Zhou, Q., Li, Q., Sun, X."Endogenous carbon monoxide downregulates hepatic cystathionine-γ-lyase in rats with liver cirrhosis". Experimental and Therapeutic Medicine 10.6 (2015): 2039-2046.
Chicago
Guo, S., Duan, Z., Wang, Q., Zhou, Q., Li, Q., Sun, X."Endogenous carbon monoxide downregulates hepatic cystathionine-γ-lyase in rats with liver cirrhosis". Experimental and Therapeutic Medicine 10, no. 6 (2015): 2039-2046. https://doi.org/10.3892/etm.2015.2823