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Isolation and characterization of side population cells from the human ovarian cancer cell line SK-OV-3

  • Authors:
    • Zhengyi Ruan
    • Jianhua Liu
    • Yanping Kuang
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China, Department of Assisted Reproduction, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China
    Copyright: © Ruan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2071-2078
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    Published online on: October 30, 2015
       https://doi.org/10.3892/etm.2015.2836
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Abstract

Ovarian cancer (OC) is the most malignant type of gynecological tumor due to its high recurrence rate following initial treatment. Previous studies have indicated that cancer stem cells (CSCs) may be a potential cause underlying the high proportion of recurrence. Side population (SP) cells isolated from cancer cell lines have been shown to exhibit characteristics associated with CSCs, but studies on SP cells in human ovarian SK‑OV‑3 cell line are limited. In the present study, the SP cell fraction (4.83% of the total cell population) was isolated using flow cytometry, and analyzed by immunocytochemical analysis and reverse transcription‑quantitative polymerase chain reaction. The results showed that SP cells exhibited a high mean fluorescence intensity for CD44, a CSC marker, in addition to elevated expression of the CSCs‑associated genes, ATP‑binding cassette sub‑family G member 2 and Nestin. These findings indicated the stem cell‑like features of the SP cells. Furthermore, a colony formation test showed that the isolated SP cells possessed a marked capacity for self‑regeneration and proliferation. In addition, a cell cycle assay involving cisplatin indicated that the SP cells were strongly resistant to chemotherapy. In conclusion, the present results suggested that SP cells isolated from the SK‑OV‑3 cell line exhibited properties typically associated with CSCs. Therefore, the isolated SP cells may be used to provide novel insight into potential therapies against OC.
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Copy and paste a formatted citation
Spandidos Publications style
Ruan Z, Liu J and Kuang Y: Isolation and characterization of side population cells from the human ovarian cancer cell line SK-OV-3. Exp Ther Med 10: 2071-2078, 2015.
APA
Ruan, Z., Liu, J., & Kuang, Y. (2015). Isolation and characterization of side population cells from the human ovarian cancer cell line SK-OV-3. Experimental and Therapeutic Medicine, 10, 2071-2078. https://doi.org/10.3892/etm.2015.2836
MLA
Ruan, Z., Liu, J., Kuang, Y."Isolation and characterization of side population cells from the human ovarian cancer cell line SK-OV-3". Experimental and Therapeutic Medicine 10.6 (2015): 2071-2078.
Chicago
Ruan, Z., Liu, J., Kuang, Y."Isolation and characterization of side population cells from the human ovarian cancer cell line SK-OV-3". Experimental and Therapeutic Medicine 10, no. 6 (2015): 2071-2078. https://doi.org/10.3892/etm.2015.2836
Copy and paste a formatted citation
x
Spandidos Publications style
Ruan Z, Liu J and Kuang Y: Isolation and characterization of side population cells from the human ovarian cancer cell line SK-OV-3. Exp Ther Med 10: 2071-2078, 2015.
APA
Ruan, Z., Liu, J., & Kuang, Y. (2015). Isolation and characterization of side population cells from the human ovarian cancer cell line SK-OV-3. Experimental and Therapeutic Medicine, 10, 2071-2078. https://doi.org/10.3892/etm.2015.2836
MLA
Ruan, Z., Liu, J., Kuang, Y."Isolation and characterization of side population cells from the human ovarian cancer cell line SK-OV-3". Experimental and Therapeutic Medicine 10.6 (2015): 2071-2078.
Chicago
Ruan, Z., Liu, J., Kuang, Y."Isolation and characterization of side population cells from the human ovarian cancer cell line SK-OV-3". Experimental and Therapeutic Medicine 10, no. 6 (2015): 2071-2078. https://doi.org/10.3892/etm.2015.2836
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