Neurolytic celiac plexus block enhances skeletal muscle insulin signaling and attenuates insulin resistance in GK rats

  • Authors:
    • Jun Li
    • Tao Chen
    • Kun Li
    • Hongtao Yan
    • Xiaowei Li
    • Yun Yang
    • Yulan Zhang
    • Bingyin Su
    • Fuxiang Li
  • View Affiliations

  • Published online on: February 19, 2016     https://doi.org/10.3892/etm.2016.3087
  • Pages: 2033-2041
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Abstract

Non‑insulin‑dependent diabetes mellitus (NIDDM) is associated with chronic inflammatory activity and disrupted insulin signaling, leading to insulin resistance (IR). The present study investigated the benefits of neurolytic celiac plexus block (NCPB) on IR in a rat NIDDM model. Goto‑Kakizaki rats fed a high‑fat, high‑glucose diet to induce signs of NIDDM were randomly divided into NCPB and control groups; these received daily bilateral 0.5% lidocaine or 0.9% saline injections into the celiac plexus, respectively. Following 14 and 28 daily injections, rats were subject to oral glucose tolerance tests (OGTTs) or sacrificed for the analysis of serum free fatty acids (FFAs), serum inflammatory cytokines and skeletal muscle insulin signaling. Compared with controls, rats in the NCPB group demonstrated significantly (P<0.05) lower baseline, 60‑min and 120‑min OGTT values, lower 120‑min serum insulin, lower IR [higher insulin sensitivity index (ISI1) and lower ISI2) and lower serum FFAs, tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑6. Conversely, NCPB rats exhibited higher basal and insulin‑stimulated skeletal muscle glucose uptake and higher skeletal muscle insulin receptor substrate‑1 (IRS‑1) and glucose transporter type 4 expression. There were no differences between the groups in insulin receptor β (Rβ) or Akt expression; however Rβ‑Y1162/Y1163 and Akt‑S473 phosphorylation levels were higher and IRS‑1‑S307 phosphorylation were lower in NCPB rats than in the controls. These results indicate that NCPB improved insulin signaling and reduced IR, possibly by inhibiting inflammatory cytokine release.
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May-2016
Volume 11 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li J, Chen T, Li K, Yan H, Li X, Yang Y, Zhang Y, Su B and Li F: Neurolytic celiac plexus block enhances skeletal muscle insulin signaling and attenuates insulin resistance in GK rats. Exp Ther Med 11: 2033-2041, 2016.
APA
Li, J., Chen, T., Li, K., Yan, H., Li, X., Yang, Y. ... Li, F. (2016). Neurolytic celiac plexus block enhances skeletal muscle insulin signaling and attenuates insulin resistance in GK rats. Experimental and Therapeutic Medicine, 11, 2033-2041. https://doi.org/10.3892/etm.2016.3087
MLA
Li, J., Chen, T., Li, K., Yan, H., Li, X., Yang, Y., Zhang, Y., Su, B., Li, F."Neurolytic celiac plexus block enhances skeletal muscle insulin signaling and attenuates insulin resistance in GK rats". Experimental and Therapeutic Medicine 11.5 (2016): 2033-2041.
Chicago
Li, J., Chen, T., Li, K., Yan, H., Li, X., Yang, Y., Zhang, Y., Su, B., Li, F."Neurolytic celiac plexus block enhances skeletal muscle insulin signaling and attenuates insulin resistance in GK rats". Experimental and Therapeutic Medicine 11, no. 5 (2016): 2033-2041. https://doi.org/10.3892/etm.2016.3087