Transplantation of mesenchymal stem cells promotes the functional recovery of the central nervous system following cerebral ischemia by inhibiting myelin‑associated inhibitor expression and neural apoptosis

Feng,Nianping; Hao,Guang; Yang,Fenggang; Qu,Fujun; Zheng,Haihong; Liang,Songlan; Jin,Yonghua

doi:10.3892/etm.2016.3089

Transplantation of mesenchymal stem cells promotes the functional recovery of the central nervous system following cerebral ischemia by inhibiting myelin‑associated inhibitor expression and neural apoptosis

Authors:
- Nianping Feng
- Guang Hao
- Fenggang Yang
- Fujun Qu
- Haihong Zheng
- Songlan Liang
- Yonghua Jin
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Affiliations: Department of Neurology, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Department of Neurology, First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei 075000, P.R. China, Department of Neurology, Central Hospital of Taian, Taian, Shandong 271000, P.R. China, Department of Pharmacy, Second Clinical Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Animal Experiment Center, Second Clinical Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China
Published online on: February 19, 2016 https://doi.org/10.3892/etm.2016.3089
Pages: 1595-1600
Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cerebral ischemia, which may lead to cerebral hypoxia and damage of the brain tissue, is a leading cause of human mortality and adult disability. Mesenchymal stem cells (MSCs) are a class of adult progenitor cells with the ability to differentiate into multiple cell types. The transplantation of bone marrow‑derived MSCs is a potential therapeutic strategy for cerebral ischemia. However, the underlying mechanism has yet to be elucidated. In the present study, primary MSCs were isolated from healthy rats, labeled and transplanted into the brains of middle cerebral artery occlusion rat models. The location of the labeled MSCs in the rat brains were determined by fluorescent microscopy, and the neurological functions of the rats were scored. Immunohistochemical analyses demonstrated that the protein expression levels of myelin‑associated inhibitors of regeneration, including Nogo‑A, oligodendrocyte myelin glycoprotein and myelin‑associated glycoprotein, were decreased following transplantation of the bone marrow‑derived MSCs. Furthermore, the mRNA expression levels of Capase‑3 and B‑cell lymphoma 2, as determined by reverse transcription‑quantitative polymerase chain reactions, were downregulated and upregulated, respectively, in the MSC‑transplanted rats; thus suggesting that neural apoptosis was inhibited. The results of the present study suggested that the transplantation of bone marrow‑derived MSCs was able to promote the functional recovery of the central nervous system following cerebral ischemia. Accordingly, inhibitors targeting myelin‑associated inhibitors and apoptosis may be of clinical significance for cerebral ischemia in the future.

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