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Selected gene profiles of stressed NSC‑34 cells and rat spinal cord following peripheral nerve reconstruction and minocycline treatment

  • Authors:
    • Gerburg Keilhoff
    • Benjamin Lucas
    • Katja Uhde
    • Hisham Fansa
  • View Affiliations / Copyright

    Affiliations: Institute of Biochemistry and Cell Biology, Otto‑Von‑Guericke University Magdeburg, Magdeburg D‑39120, Germany, Department of Plastic, Reconstructive and Aesthetic Surgery, Hand Surgery, Klinikum Bielefeld, Bielefeld D‑33604, Germany
    Copyright: © Keilhoff et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1685-1699
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    Published online on: March 2, 2016
       https://doi.org/10.3892/etm.2016.3130
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Abstract

The present study was conducted to investigate the effects of minocycline on the expression of selected transcriptional and translational profiles in the rat spinal cord following sciatic nerve (SNR) transection and microsurgical coaptation. The mRNA and protein expression levels of B cell lymphoma‑2 (Bcl‑2), Bcl‑2‑associated X protein (Bax), caspase‑3, major histocompatibility complex I (MHC I), tumor necrosis factor‑α (TNF‑α), activating transcription factor 3 (ATF3), vascular endothelial growth factor (VEGF), matrix metalloproteinase 9 (MMP9), and growth associated protein‑43 (GAP‑43) were monitored in the rat lumbar spinal cord following microsurgical reconstruction of the sciatic nerves and minocycline treatment. The present study used semi‑quantitative reverse transcription‑polymerase chain reaction (RT‑PCR) and immunohistochemistry. As a PCR analysis of spinal cord tissue enabled the examination of the expression patterns of all cell types including glia, the motorneuron‑like NSC‑34 cell line was used to investigate expression level changes in motorneurons. As stressors, oxygen glucose deprivation (OGD) and lipopolysaccharide (LPS) treatment were performed. SNR did not induce significant degeneration of ventral horn motorneurons, whereas microglia activation and synaptic terminal retraction were detectable. All genes were constitutively expressed at the mRNA and protein levels in untreated spinal cord and control cells. SNR significantly increased the mRNA expression levels of all genes, albeit only temporarily. In all genes except MMP9 and GAP‑43, the induction was seen ipsilaterally and contralaterally. The effects of minocycline were moderate. The expression levels of MMP9, TNF‑α, MHC I, VEGF, and GAP‑43 were reduced, whereas those of Bax and Bcl‑2 were unaffected. OGD, but not LPS, was toxic for NSC‑34 cells. No changes in the expression levels of Bax, caspase‑3, MHC I or ATF3 were observed. These results indicated that motorneurons were not preferentially or solely responsible for SNR‑mediated upregulation of these genes. MMP9, TNF‑α, VEGF and Bcl‑2 were stress‑activated. These results suggest that a substantial participation of motorneurons in gene expression levels in vivo. Minocycline was also shown to have inhibitory effects. The nuclear factor‑κB signalling pathway may be a possible target of minocycline.
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1 

Oliveira AL, Risling M, Deckner M, Lindholm T, Langone F and Cullheim S: Neonatal sciatic nerve transection induces TUNEL labeling of neurons in the rat spinal cord and DRG. Neuroreport. 8:2837–2840. 1997. View Article : Google Scholar : PubMed/NCBI

2 

Chen LJ, Ren YH, Liu L, Zhang XQ, Zhao Y, Wu WT and Li F: Upregulated expression of GAP-43 mRNA and protein in anterior horn motoneurons of the spinal cord after brachial plexus injury. Arch Med Res. 41:513–538. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Arocho LC, Figueroa JD, Torrado AI, Santiago JM, Vera AE and Miranda JD: Expression profile and role of EphrinA1 ligand after spinal cord injury. Cell Mol Neurobiol. 31:1057–1069. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Kobbert C and Thanos S: Topographic representation of the sciatic nerve motor neurons in the spinal cord of the adult rat correlates to region-specific activation patterns of microglia. J Neurocytol. 29:271–283. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Zheng LF, Wang R, Xu YZ, Yi XN, Zhang JW and Zeng ZC: Calcitonin gene-related peptide dynamics in rat dorsal root ganglia and spinal cord following different sciatic nerve injuries. Brain Res. 1187:20–32. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Wu F, Miao X, Chen J, Sun Y, Liu Z, Tao Y and Yu W: Down-regulation of GAP-43 by inhibition of caspases-3 in a rat model of neuropathic pain. Int J Clin Exp Pathol. 5:948–955. 2012.PubMed/NCBI

7 

Terenghi G, Hart A and Wiberg M: The nerve injury and the dying neurons: Diagnosis and prevention. J Hand Surg Eur Vol. 36:730–734. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Amantea D and Bagetta G: Drug repurposing for immune modulation in acute ischemic stroke. Curr Opin Pharmacol. 26:124–130. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Smilack JD: The tetracyclines. Mayo Clin Proc. 74:727–729. 1999. View Article : Google Scholar : PubMed/NCBI

10 

Yrjanheikki J, Tikka T, Keinänen R, Goldsteins G, Chan PH and Koistinaho J: A tetracycline derivative, minocycline, reduces inflammation and protects against focal cerebral ischemia with a wide therapeutic window. Proc Natl Acad Sci USA. 96:13496–13500. 1999. View Article : Google Scholar : PubMed/NCBI

11 

Xu L, Fagan SC, Waller JL, Edwards D, Borlongan CV, Zheng J, Hill WD, Feuerstein G and Hess DC: Low dose intravenous minocycline is neuroprotective after middle cerebral artery occlusion-reperfusion in rats. BMC Neurol. 4:72004. View Article : Google Scholar : PubMed/NCBI

12 

Chen SD, Yin JH, Hwang CS, Tang CM and Yang DI: Anti-apoptotic and anti-oxidative mechanisms of minocycline against sphingomyelinase/ceramide neurotoxicity: Implication in Alzheimer's disease and cerebral ischemia. Free Radic Res. 46:340–350. 2012. View Article : Google Scholar

13 

Hodl AK and Bonelli RM: Huntington's disease and minocycline. Mov Disord. 20:510–511. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Zemke D and Majid A: The potential of minocycline for neuroprotection in human neurologic disease. Clin Neuropharmacol. 27:293–298. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Pontieri FE, Ricci A, Pellicano C, Benincasa D and Buttarelli FR: Minocycline in amyotrophic lateral sclerosis: A pilot study. Neurol Sci. 26:285–287. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Giuliani F, Fu SA, Metz LM and Yong VW: Effective combination of minocycline and interferon-beta in a model of multiple sclerosis. J Neuroimmunol. 165:83–91. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Wells JE, Hurlbert RJ, Fehlings MG and Yong VW: Neuroprotection by minocycline facilitates significant recovery from spinal cord injury in mice. Brain. 126:1628–1637. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Monaco EA 3rd, Weiner GM and Friedlander RM: Randomized-controlled trial of minocycline for spinal cord injury shows promise. Neurosurgery. 72:N17–19. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Stirling DP, Koochesfahani KM, Steeves JD and Tetzlaff W: Minocycline as a neuroprotective agent. Neuroscientist. 11:308–322. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Diguet E, Gross CE, Tison F and Bezard E: Rise and fall of minocycline in neuroprotection: Need to promote publication of negative results. Exp Neurol. 189:1–4. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Tsuji M, Wilson MA, Lange MS and Johnston MV: Minocycline worsens hypoxic-ischemic brain injury in a neonatal mouse model. Exp Neurol. 189:58–65. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Pinzon A, Marcillo A, Quintana A, Stamler S, Bunge MB, Bramlett HM and Dietrich WD: A re-assessment of minocycline as a neuroprotective agent in a rat spinal cord contusion model. Brain Res. 1243:146–151. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Lee JH, Tigchelaar S, Liu J, Stammers AM, Streijger F, Tetzlaff W and Kwon BK: Lack of neuroprotective effects of simvastatin and minocycline in a model of cervical spinal cord injury. Exp Neurol. 225:219–230. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Pinkernelle J, Fansa H, Ebmeyer U and Keilhoff G: Prolonged minocycline treatment impairs motor neuronal survival and glial function in organotypic rat spinal cord cultures. PloS One. 8:e734222013. View Article : Google Scholar : PubMed/NCBI

25 

Keilhoff G, Langnaese K, Wolf G and Fansa H: Inhibiting effect of minocycline on the regeneration of peripheral nerves. Dev Neurobiol. 67:1382–1395. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Cashman NR, Durham HD, Blusztajn JK, Oda K, Tabira T, Shaw IT, Dahrouge S and Antel JP: Neuroblastoma × spinal cord (NSC) hybrid cell lines resemble developing motor neurons. Dev Dyn. 194:209–221. 1992. View Article : Google Scholar : PubMed/NCBI

27 

Tiraihi T and Rezaie MJ: Apoptosis onset and bax protein distribution in spinal motoneurons of newborn rats following sciatic nerve axotomy. Int J Neurosci. 113:1163–1175. 2003. View Article : Google Scholar : PubMed/NCBI

28 

Zhao W, Zhao Q, Liu J, Xu XY, Sun WW, Zhou X, Liu S and Wang TH: Electro-acupuncture reduces neuronal apoptosis linked to Bax and Bcl-2 expression in the spinal cords of cats subjected to partial dorsal root ganglionectomy. Neurochem Res. 33:2214–2221. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Martin LJ and Liu Z: Injury-induced spinal motor neuron apoptosis is preceded by DNA single-strand breaks and is p53- and Bax-dependent. J Neurobiol. 50:181–197. 2002. View Article : Google Scholar : PubMed/NCBI

30 

Lu XM, Shu YH, Qiu CH, Chen KT and Wang YT: Protective effects and anti-apoptotic role of nerve growth factor on spinal cord neurons in sciatic nerve-injured rats. Neurol Res. 36:814–823. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Payés AC, Zanon RG, Pierucci A and Oliveira AL: MHC class I upregulation is not sufficient to rescue neonatal alpha motoneurons after peripheral axotomy. Brain Res. 1238:23–30. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Ohtori S, Takahashi K, Moriya H and Myers RR: TNF-alpha and TNF-alpha receptor type 1 upregulation in glia and neurons after peripheral nerve injury: Studies in murine DRG and spinal cord. Spine (Phila Pa 1976). 29:1082–1088. 2004. View Article : Google Scholar : PubMed/NCBI

33 

Lindå H, Sköld MK and Ochsmann T: Activating transcription factor 3, a useful marker for regenerative response after nerve root injury. Front Neurol. 2:302011. View Article : Google Scholar : PubMed/NCBI

34 

Hunt D, Raivich G and Anderson PN: Activating transcription factor 3 and the nervous system. Front Mol Neurosci. 5:72012. View Article : Google Scholar : PubMed/NCBI

35 

Fu CY, Hong GX and Wang FB: Expression of vascular endothelial growth factor and its fetal liver kinase-1 receptor in spinal cord and dorsal root ganglia after neurotomy of sciatic nerve in rats. Chin J Traumatol. 8:17–22. 2005.PubMed/NCBI

36 

Liou JT, Sum DC, Liu FC, Mao CC, Lai YS and Day YJ: Spatial and temporal analysis of nociception-related spinal cord matrix metalloproteinase expression in a murine neuropathic pain model. J Chin Med Assoc. 76:201–110. 2013. View Article : Google Scholar : PubMed/NCBI

37 

Jacobson RD, Virág I and Skene JH: A protein associated with axon growth, GAP-43, is widely distributed and developmentally regulated in rat CNS. J Neurosci. 6:1843–1855. 1986.PubMed/NCBI

38 

Bulsara KR, Iskandar BJ, Villavicencio AT and Skene JH: A new millenium for spinal cord regeneration: Growth-associated genes. Spine (Phila Pa 1976). 27:1946–1949. 2002. View Article : Google Scholar : PubMed/NCBI

39 

Raghavendra V, Tanga F and DeLeo JA: Inhibition of microglial activation attenuates the development but not existing hypersensitivity in a rat model of neuropathy. J Pharmacol Exp Ther. 306:624–630. 2003. View Article : Google Scholar : PubMed/NCBI

40 

Langnaese K, John R, Schweizer H, Ebmeyer U and Keilhoff G: Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model. BMC Mol Biol. 9:532008. View Article : Google Scholar : PubMed/NCBI

41 

Lucas B, Pinkernelle J, Fansa H and Keilhoff G: Effects of cerebrolysin on rat Schwann cells in vitro. Acta Histochem. 116:820–830. 2014. View Article : Google Scholar : PubMed/NCBI

42 

Keilhoff G and Wolf G: Comparison of double fluorescence staining and LDH-test for monitoring cell viability in vitro. Neuroreport. 5:129–132. 1993. View Article : Google Scholar : PubMed/NCBI

43 

Infante SK, Oberhauser AF and Perez-Polo JR: Bax phosphorylation association with nucleus and oligomerization after neonatal hypoxia-ischemia. J Neurosci Res. 91:1152–1164. 2013. View Article : Google Scholar : PubMed/NCBI

44 

Seijffers R, Mills CD and Woolf CJ: ATF3 increases the intrinsic growth state of DRG neurons to enhance peripheral nerve regeneration. J Neurosci. 27:7911–7920. 2007. View Article : Google Scholar : PubMed/NCBI

45 

Wolff JR and Missler M: Synaptic reorganization in developing and adult nervous systems. Ann Anat. 174:393–403. 1992. View Article : Google Scholar : PubMed/NCBI

46 

Vieira AS, de Rezende AC and Rogerio F: Evaluating motor neuron death in neonatal rats subjected to sciatic nerve lesion. Methods Mol Biol. 846:383–391. 2012. View Article : Google Scholar : PubMed/NCBI

47 

Carlson J, Lais AC and Dyck PJ: Axonal atrophy from permanent peripheral axotomy in adult cat. J Neuropathol Exp Neurol. 38:579–585. 1979. View Article : Google Scholar : PubMed/NCBI

48 

Victório SC, Havton LA and Oliveira AL: Absence of IFNγ expression induces neuronal degeneration in the spinal cord of adult mice. J Neuroinflammation. 7:772010. View Article : Google Scholar : PubMed/NCBI

49 

Ohlsson M, Nieto JH, Christe KL and Havton LA: Long-term effects of a lumbosacral ventral root avulsion injury on axotomized motor neurons and avulsed ventral roots in a non-human primate model of cauda equina injury. Neuroscience. 250:129–139. 2013. View Article : Google Scholar : PubMed/NCBI

50 

Chen T, Koga K, Li XY and Zhuo M: Spinal microglial motility is independent of neuronal activity and plasticity in adult mice. Mol Pain. 6:192010. View Article : Google Scholar : PubMed/NCBI

51 

Chew DJ, Carlstedt T and Shortland PJ: A comparative histological analysis of two models of nerve root avulsion injury in the adult rat. Neuropathol Appl Neurobiol. 37:613–632. 2011. View Article : Google Scholar : PubMed/NCBI

52 

Hart AM, Terenghi G and Wiberg M: Neuronal death after peripheral nerve injury and experimental strategies for neuroprotection. Neurol Res. 30:999–1011. 2008. View Article : Google Scholar : PubMed/NCBI

53 

Gillardon F, Klimaschewski L, Wickert H, Krajewski S, Reed JC and Zimmermann M: Expression pattern of candidate cell death effector proteins Bax, Bcl-2, Bcl-X and c-Jun in sensory and motor neurons following sciatic nerve transection in the rat. Brain Res. 739:244–250. 1996. View Article : Google Scholar : PubMed/NCBI

54 

Tsujino H, Kondo E, Fukuoka T, Dai Y, Tokunaga A, Miki K, Yonenobu K, Ochi T and Noguchi K: Activating transcription factor 3 (ATF3) induction by axotomy in sensory and motoneurons: A novel neuronal marker of nerve injury. Mol Cell Neurosci. 15:170–182. 2000. View Article : Google Scholar : PubMed/NCBI

55 

Sabha M Jr, Emirandetti A, Cullheim S and De Oliveira AL: MHC I expression and synaptic plasticity in different mice strains after axotomy. Synapse. 62:137–148. 2008. View Article : Google Scholar : PubMed/NCBI

56 

Schwartz M, Butovsky O, Brück W and Hanisch UK: Microglial phenotype: Is the commitment reversible? Trends Neurosci. 29:68–74. 2006. View Article : Google Scholar : PubMed/NCBI

57 

Vega-Avelaira D, Moss A and Fitzgerald M: Age-related changes in the spinal cord microglial and astrocytic response profile to nerve injury. Brain Behav Immun. 21:617–623. 2007. View Article : Google Scholar : PubMed/NCBI

58 

Tang Y, Ling ZM, Fu R, Li YQ, Cheng X, Song FH, Luo HX and Zhou LH: Time-specific microRNA changes during spinal motoneuron degeneration in adult rats following unilateral brachial plexus root avulsion: Ipsilateral vs. contralateral changes. BMC Neurosci. 15:922014. View Article : Google Scholar : PubMed/NCBI

59 

Rotshenker S and Tal M: The transneuronal induction of sprouting and synapse formation in intact mouse muscles. J Physiol. 360:387–396. 1985. View Article : Google Scholar : PubMed/NCBI

60 

Rotto-Percelay DM, Wheeler JG, Osorio FA, Platt KB and Loewy AD: Transneuronal labeling of spinal interneurons and sympathetic preganglionic neurons after pseudorabies virus injections in the rat medial gastrocnemius muscle. Brain Res. 574:291–306. 1992. View Article : Google Scholar : PubMed/NCBI

61 

Villar MJ, Cortés R, Theodorsson E, Wiesenfeld-Hallin Z, Schalling M, Fahrenkrug J, Emson PC and Hökfelt T: Neuropeptide expression in rat dorsal root ganglion cells and spinal cord after peripheral nerve injury with special reference to galanin. Neuroscience. 33:587–604. 1989. View Article : Google Scholar : PubMed/NCBI

62 

Piehl F, Arvidsson U, Johnson H, Cullheim S, Villar M, Dagerlind A, Terenius L, Hökfelt T and Ulfhake B: Calcitonin Gene-related Peptide (CGRP)-like Immunoreactivity and CGRP mRNA in rat spinal cord motoneurons after different types of lesions. Eur J Neurosci. 3:737–757. 1991. View Article : Google Scholar : PubMed/NCBI

63 

Benemei S, Nicoletti P, Capone JG and Geppetti P: CGRP receptors in the control of pain and inflammation. Curr Opin Pharmacol. 9:9–14. 2009. View Article : Google Scholar : PubMed/NCBI

64 

Sueur S, Pesant M, Rochette L and Connat JL: Antiapoptotic effect of calcitonin gene-related peptide on oxidative stress-induced injury in H9c2 cardiomyocytes via the RAMP1/CRLR complex. J Mol Cell Cardiol. 39:955–963. 2005. View Article : Google Scholar : PubMed/NCBI

65 

Spejo AB and Oliveira AL: Synaptic rearrangement following axonal injury: Old and new players. Neuropharmacology. 96:113–123. 2015. View Article : Google Scholar : PubMed/NCBI

66 

Guntinas-Lichius O, Neiss WF, Gunkel A and Stennert E: Differences in glial, synaptic and motoneuron responses in the facial nucleus of the rat brainstem following facial nerve resection and nerve suture reanastomosis. Eur Arch Otorhinolaryngol. 251:410–417. 1994. View Article : Google Scholar : PubMed/NCBI

67 

Hardingham GE: Coupling of the NMDA receptor to neuroprotective and neurodestructive events. Biochem Soc Trans. 37:1147–1160. 2009. View Article : Google Scholar : PubMed/NCBI

68 

Tyzack GE, Sitnikov S, Barson D, Adams-Carr KL, Lau NK, Kwok JC, Zhao C, Franklin RJ, Karadottir RT, Fawcett JW and Lakatos A: Astrocyte response to motor neuron injury promotes structural synaptic plasticity via STAT3-regulated TSP-1 expression. Nat Commun. 5:42942014. View Article : Google Scholar : PubMed/NCBI

69 

Garrido-Mesa N, Zarzuelo A and Gálvez J: Minocycline: Far beyond an antibiotic. Br J Pharmacol. 169:337–352. 2013. View Article : Google Scholar : PubMed/NCBI

70 

Liao TV, Forehand CC, Hess DC and Fagan SC: Minocycline repurposing in critical illness: Focus on stroke. Curr Top Med Chem. 13:2283–2290. 2013. View Article : Google Scholar : PubMed/NCBI

71 

Li C, Yuan K and Schluesener H: Impact of minocycline on neurodegenerative diseases in rodents: A meta-analysis. Rev Neurosci. 24:553–562. 2013. View Article : Google Scholar : PubMed/NCBI

72 

Teng YD, Choi H, Onario RC, Zhu S, Desilets FC, Lan S, Woodard EJ, Snyder EY, Eichler ME and Friedlander RM: Minocycline inhibits contusion-triggered mitochondrial cytochrome c release and mitigates functional deficits after spinal cord injury. Proc Natl Acad Sci USA. 101:3071–3076. 2004. View Article : Google Scholar : PubMed/NCBI

73 

Chaturvedi M and Kaczmarek L: Mmp-9 inhibition: A therapeutic strategy in ischemic stroke. Mol Neurobiol. 49:563–673. 2014. View Article : Google Scholar : PubMed/NCBI

74 

Enose-Akahata Y, Matsuura E, Tanaka Y, Oh U and Jacobson S: Minocycline modulates antigen-specific CTL activity through inactivation of mononuclear phagocytes in patients with HTLV-I associated neurologic disease. Retrovirology. 9:162012. View Article : Google Scholar : PubMed/NCBI

75 

Jung HJ, Seo I, Jha BK, Suh SI, Suh MH and Baek WK: Minocycline inhibits angiogenesis in vitro through the translational suppression of HIF-1α. Arch Biochem Biophys. 545:74–82. 2014. View Article : Google Scholar : PubMed/NCBI

76 

Li CH, Liao PL, Yang YT, Huang SH, Lin CH, Cheng YW and Kang JJ: Minocycline accelerates hypoxia-inducible factor-1 alpha degradation and inhibits hypoxia-induced neovasculogenesis through prolyl hydroxylase, von Hippel-Lindau-dependent pathway. Arch Toxicol. 88:659–671. 2014.PubMed/NCBI

77 

Sun C, Li XX, He XJ, Zhang Q and Tao Y: Neuroprotective effect of minocycline in a rat model of branch retinal vein occlusion. Exp Eye Res. 113:105–116. 2013. View Article : Google Scholar : PubMed/NCBI

78 

Levkovitz Y, Fenchel D, Kaplan Z, Zohar J and Cohen H: Early post-stressor intervention with minocycline, a second-generation tetracycline, attenuates post-traumatic stress response in an animal model of PTSD. Eur Neuropsychopharmacol. 25:124–132. 2015. View Article : Google Scholar : PubMed/NCBI

79 

Kwon MJ, Kim J, Shin H, Jeong SR, Kang YM, Choi JY, Hwang DH and Kim BG: Contribution of macrophages to enhanced regenerative capacity of dorsal root ganglia sensory neurons by conditioning injury. J Neurosci. 33:15095–15108. 2013. View Article : Google Scholar : PubMed/NCBI

80 

Ataie-Kachoie P, Badar S, Morris DL and Pourgholami MH: Minocycline targets the NF-kB Nexus through suppression of TGF-β1-TAK1-IkB signaling in ovarian cancer. Mol Cancer Res. 11:1279–1291. 2013. View Article : Google Scholar : PubMed/NCBI

81 

Pang T, Wang J, Benicky J and Saavedra JM: Minocycline ameliorates LPS-induced inflammation in human monocytes by novel mechanisms including LOX-1, Nur77 and LITAF inhibition. Biochim Biophys Acta. 1820:503–510. 2012. View Article : Google Scholar : PubMed/NCBI

82 

Li H, Xu H and Sun B: Lipopolysaccharide regulates MMP-9 expression through TLR4/NF-kB signaling in human arterial smooth muscle cells. Mol Med Rep. 6:774–778. 2012.PubMed/NCBI

83 

Pick M, Ronen D, Yanuka O and Benvenisty N: Reprogramming of the MHC-I and its regulation by NFkB in human-induced pluripotent stem cells. Stem Cells. 30:2700–2708. 2012. View Article : Google Scholar : PubMed/NCBI

84 

Xie TX, Xia Z, Zhang N, Gong W and Huang S: Constitutive NF-kB activity regulates the expression of VEGF and IL-8 and tumor angiogenesis of human glioblastoma. Oncol Rep. 23:725–732. 2010.PubMed/NCBI

85 

Zhao M, Zhou A, Xu L and Zhang X: The role of TLR4-mediated PTEN/PI3K/AKT/NF-kB signaling pathway in neuroinflammation in hippocampal neurons. Neuroscience. 269:93–101. 2014. View Article : Google Scholar : PubMed/NCBI

86 

Pozniak PD, White MK and Khalili K: TNF-α/NF-κB signaling in the CNS: Possible connection to EPHB2. J Neuroimmune Pharmacol. 9:133–141. 2014. View Article : Google Scholar : PubMed/NCBI

87 

Matsuura Y, Ochi M, Uchio Y, Suzuki G and Iwata A: The time-dependent difference of GAP-43 expression between sensory neurons and motoneurons after peripheral nerve transection. Scand J Plast Reconstr Surg Hand Surg. 33:267–272. 1999. View Article : Google Scholar : PubMed/NCBI

88 

Gordon T, You S, Cassar SL and Tetzlaff W: Reduced expression of regeneration associated genes in chronically axotomized facial motoneurons. Exp Neurol. 264:26–32. 2015. View Article : Google Scholar : PubMed/NCBI

89 

Tedeschi A, Nguyen T, Puttagunta R, Gaub P and Di Giovanni S: A p53-CBP/p300 transcription module is required for GAP-43 expression, axon outgrowth and regeneration. Cell Death Differ. 16:543–554. 2009. View Article : Google Scholar : PubMed/NCBI

90 

Jiang BP, Le L, Xu LJ and Xiao PG: Minocycline inhibits ICAD degradation and the NF-kB activation induced by 6-OHDA in PC12 cells. Brain Res. 1586:1–11. 2014. View Article : Google Scholar : PubMed/NCBI

91 

Kadiyala CS, Zheng L, Du Y, Yohannes E, Kao HY, Miyagi M and Kern TS: Acetylation of retinal histones in diabetes increases inflammatory proteins: Effects of minocycline and manipulation of histone acetyltransferase (HAT) and histone deacetylase (HDAC). J Biol Chem. 287:25869–25880. 2012. View Article : Google Scholar : PubMed/NCBI

92 

Levkovitch-Verbin H, Waserzoog Y, Vander S, Makarovsky D and Piven I: Minocycline upregulates pro-survival genes and downregulates pro-apoptotic genes in experimental glaucoma. Graefes Arch Clin Exp Ophthalmol. 252:761–772. 2014. View Article : Google Scholar : PubMed/NCBI

93 

Hassanzadeh K, Habibi-asl B, Farajnia S and Roshangar L: Minocycline prevents morphine-induced apoptosis in rat cerebral cortex and lumbar spinal cord: A possible mechanism for attenuating morphine tolerance. Neurotox Res. 19:649–659. 2011. View Article : Google Scholar : PubMed/NCBI

94 

Filipovic R and Zecevic N: Neuroprotective role of minocycline in co-cultures of human fetal neurons and microglia. Exp Neurol. 211:41–51. 2008. View Article : Google Scholar : PubMed/NCBI

95 

Matsukawa N, Yasuhara T, Hara K, Xu L, Maki M, Yu G, Kaneko Y, Ojika K, Hess DC and Borlongan CV: Therapeutic targets and limits of minocycline neuroprotection in experimental ischemic stroke. BMC Neurosci. 10:1262009. View Article : Google Scholar : PubMed/NCBI

96 

Kerr DA, Larsen T, Cook SH, Fannjiang YR, Choi E, Griffin DE, Hardwick JM and Irani DN: BCL-2 and BAX protect adult mice from lethal Sindbis virus infection but do not protect spinal cord motor neurons or prevent paralysis. J Virol. 76:10393–10400. 2002. View Article : Google Scholar : PubMed/NCBI

97 

Hemendinger RA, Armstrong EJ 3rd, Radio N and Brooks BR: Neurotoxic injury pathways in differentiated mouse motor neuron-neuroblastoma hybrid (NSC-34D) cells in vitro - limited effect of riluzole on thapsigargin, but not staurosporine, hydrogen peroxide and homocysteine neurotoxicity. Toxicol Appl Pharmacol. 258:208–215. 2012. View Article : Google Scholar : PubMed/NCBI

98 

Soo KY, Atkin JD, Horne MK and Nagley P: Recruitment of mitochondria into apoptotic signaling correlates with the presence of inclusions formed by amyotrophic lateral sclerosis-associated SOD1 mutations. J Neurochem. 108:578–590. 2009. View Article : Google Scholar : PubMed/NCBI

99 

Lee SH, Choi NY, Yu HJ, Park J, Choi H, Lee KY, Huh YM, Lee YJ and Koh SH: Atorvastatin protects NSC-34 motor neurons against oxidative stress by activating PI3K, ERK and free radical scavenging. Mol Neurobiol. Jan 11–2015.(Epub ahead of print).

100 

Ezzi SA, Urushitani M and Julien JP: Wild-type superoxide dismutase acquires binding and toxic properties of ALS-linked mutant forms through oxidation. J Neurochem. 102:170–178. 2007. View Article : Google Scholar : PubMed/NCBI

101 

Liu YX, Tai JL, Li GQ, Zhang ZW, Xue JH, Liu HS, Zhu H, Cheng JD, Liu YL, Li AM and Zhang Y: Exposure to 1950-MHz TD-SCDMA electromagnetic fields affects the apoptosis of astrocytes via caspase-3-dependent pathway. PLoS One. 7:e423322012. View Article : Google Scholar : PubMed/NCBI

102 

Ransohoff RM and Estes ML: Astrocyte expression of major histocompatibility complex gene products in multiple sclerosis brain tissue obtained by stereotactic biopsy. Arch Neurol. 48:1244–1246. 1991. View Article : Google Scholar : PubMed/NCBI

103 

Kim KH, Jeong JY, Surh YJ and Kim KW: Expression of stress-response ATF3 is mediated by Nrf2 in astrocytes. Nucleic Acids Res. 38:48–59. 2010. View Article : Google Scholar : PubMed/NCBI

104 

Sunkaria A, Wani WY, Sharma DR and Gill KD: Dichlorvos exposure results in activation induced apoptotic cell death in primary rat microglia. Chem Res Toxicol. 25:1762–1770. 2012. View Article : Google Scholar : PubMed/NCBI

105 

Tambuyzer BR, Ponsaerts P and Nouwen EJ: Microglia: Gatekeepers of central nervous system immunology. J Leukoc Biol. 85:352–370. 2009. View Article : Google Scholar : PubMed/NCBI

106 

Simonishvili S, Jain MR, Li H, Levison SW and Wood TL: Identification of Bax-interacting proteins in oligodendrocyte progenitors during glutamate excitotoxicity and perinatal hypoxia-ischemia. ASN Neuro. 5:e001312013. View Article : Google Scholar : PubMed/NCBI

107 

Ji Q, Castelli L and Goverman JM: MHC class I-restricted myelin epitopes are cross-presented by Tip-DCs that promote determinant spreading to CD8+ T cells. Nat Immunol. 14:254–261. 2013. View Article : Google Scholar : PubMed/NCBI

108 

Goldberg J, Daniel M, van Heuvel Y, Victor M, Beyer C, Clarner T and Kipp M: Short-term cuprizone feeding induces selective amino acid deprivation with concomitant activation of an integrated stress response in oligodendrocytes. Cell Mol Neurobiol. 33:1087–1098. 2013. View Article : Google Scholar : PubMed/NCBI

109 

Johanson SO, Crouch MF and Hendry IA: Retrograde axonal transport of signal transduction proteins in rat sciatic nerve. Brain Res. 690:55–63. 1995. View Article : Google Scholar : PubMed/NCBI

110 

Wang HH, Hsieh HL, Wu CY and Yang CM: Oxidized low-density lipoprotein-induced matrix metalloproteinase-9 expression via PKC-delta/p42/p44 MAPK/Elk-1 cascade in brain astrocytes. Neurotox Res. 17:50–65. 2010. View Article : Google Scholar : PubMed/NCBI

111 

Lively S and Schlichter LC: The microglial activation state regulates migration and roles of matrix-dissolving enzymes for invasion. J Neuroinflammation. 10:752013. View Article : Google Scholar : PubMed/NCBI

112 

Monnet-Tschudi F, Defaux A, Braissant O, Cagnon L and Zurich MG: Methods to assess neuroinflammation. Curr Protoc Toxicol Unit. 12:192011.

113 

Krum JM and Khaibullina A: Inhibition of endogenous VEGF impedes revascularization and astroglial proliferation: Roles for VEGF in brain repair. Exp Neurol. 181:241–257. 2003. View Article : Google Scholar : PubMed/NCBI

114 

Shin YJ, Riew TR, Park JH, Pak HJ and Lee MY: Expression of vascular endothelial growth factor-C (VEGF-C) and its receptor (VEGFR-3) in the glial reaction elicited by human mesenchymal stem cell engraftment in the normal rat brain. J Histochem Cytochem. 63:170–180. 2015. View Article : Google Scholar : PubMed/NCBI

115 

Keshavarz M, Emamghoreishi M, Nekooeian AA, Warsh JJ and Zare HR: Increased bcl-2 protein levels in rat primary astrocyte culture following chronic lithium treatment. Iran J Med Sci. 38:255–262. 2013.PubMed/NCBI

116 

Barber SC, Higginbottom A, Mead RJ, Barber S and Shaw PJ: An in vitro screening cascade to identify neuroprotective antioxidants in ALS. Free Radic Biol Med. 46:1127–1138. 2009. View Article : Google Scholar : PubMed/NCBI

117 

Prell T, Lautenschläger J, Weidemann L, Ruhmer J, Witte OW and Grosskreutz J: Endoplasmic reticulum stress is accompanied by activation of NF-kB in amyotrophic lateral sclerosis. J Neuroimmunol. 270:29–36. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Keilhoff G, Lucas B, Uhde K and Fansa H: Selected gene profiles of stressed NSC‑34 cells and rat spinal cord following peripheral nerve reconstruction and minocycline treatment. Exp Ther Med 11: 1685-1699, 2016.
APA
Keilhoff, G., Lucas, B., Uhde, K., & Fansa, H. (2016). Selected gene profiles of stressed NSC‑34 cells and rat spinal cord following peripheral nerve reconstruction and minocycline treatment. Experimental and Therapeutic Medicine, 11, 1685-1699. https://doi.org/10.3892/etm.2016.3130
MLA
Keilhoff, G., Lucas, B., Uhde, K., Fansa, H."Selected gene profiles of stressed NSC‑34 cells and rat spinal cord following peripheral nerve reconstruction and minocycline treatment". Experimental and Therapeutic Medicine 11.5 (2016): 1685-1699.
Chicago
Keilhoff, G., Lucas, B., Uhde, K., Fansa, H."Selected gene profiles of stressed NSC‑34 cells and rat spinal cord following peripheral nerve reconstruction and minocycline treatment". Experimental and Therapeutic Medicine 11, no. 5 (2016): 1685-1699. https://doi.org/10.3892/etm.2016.3130
Copy and paste a formatted citation
x
Spandidos Publications style
Keilhoff G, Lucas B, Uhde K and Fansa H: Selected gene profiles of stressed NSC‑34 cells and rat spinal cord following peripheral nerve reconstruction and minocycline treatment. Exp Ther Med 11: 1685-1699, 2016.
APA
Keilhoff, G., Lucas, B., Uhde, K., & Fansa, H. (2016). Selected gene profiles of stressed NSC‑34 cells and rat spinal cord following peripheral nerve reconstruction and minocycline treatment. Experimental and Therapeutic Medicine, 11, 1685-1699. https://doi.org/10.3892/etm.2016.3130
MLA
Keilhoff, G., Lucas, B., Uhde, K., Fansa, H."Selected gene profiles of stressed NSC‑34 cells and rat spinal cord following peripheral nerve reconstruction and minocycline treatment". Experimental and Therapeutic Medicine 11.5 (2016): 1685-1699.
Chicago
Keilhoff, G., Lucas, B., Uhde, K., Fansa, H."Selected gene profiles of stressed NSC‑34 cells and rat spinal cord following peripheral nerve reconstruction and minocycline treatment". Experimental and Therapeutic Medicine 11, no. 5 (2016): 1685-1699. https://doi.org/10.3892/etm.2016.3130
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