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Article

Effect of lysophosphatidic acid on the immune inflammatory response and the connexin 43 protein in myocardial infarction

  • Authors:
    • Duoduo Zhang
    • Yan Zhang
    • Chunyan Zhao
    • Wenjie Zhang
    • Guoguang Shao
    • Hong Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Anesthesiology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Department of Physiology, Norman Bethune College of Medicine, Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 1617-1624
    |
    Published online on: March 9, 2016
       https://doi.org/10.3892/etm.2016.3132
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Abstract

Lysophosphatidic acid (LPA) is an intermediate product of membrane phospholipid metabolism. Recently, LPA has gained attention for its involvement in the pathological processes of certain cardiovascular diseases. The aim of the present study was to clarify the association between the effect of LPA and the immune inflammatory response, and to investigate the effects of LPA on the protein expression levels of connexin 43 during myocardial infarction. Surface electrocardiograms of myocardial infarction rats and isolated rat heart tissue samples were obtained in order to determine the effect of LPA on the incidence of arrhythmia in rats that exhibited changes in immune status. The results demonstrated that the incidence of arrhythmia decreased when the rat immune systems were suppressed, and the incidence of arrhythmia increased when the rat immune systems were enhanced. The concentration levels of tumor necrosis factor (TNF)‑α were determined by ELISA, and the results demonstrated that LPA induced T lymphocyte synthesis and TNF‑α release. Using a patch‑clamp technique, LPA was shown to increase the current amplitude of the voltage‑dependent potassium channels (Kv) and calcium‑activated potassium channels (KCa) in Jurkat T cells. The protein expression of connexin 43 (Cx43) was determined by immunohistochemical staining. The results indicated that LPA caused the degradation of Cx43 and decreased the expression of Cx43. This effect was associated with the immune status of the rats. There was a further decrease in Cx43 expression in the rats of the immune‑enhanced group. To the best of our knowledge, these results provide the first evidence that LPA causes arrhythmia through the regulation of immune inflammatory cells and the decrease of Cx43 protein expression. The present study provided an experimental basis for the treatment of arrhythmia and may guide clinical care.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang D, Zhang Y, Zhao C, Zhang W, Shao G and Zhang H: Effect of lysophosphatidic acid on the immune inflammatory response and the connexin 43 protein in myocardial infarction. Exp Ther Med 11: 1617-1624, 2016.
APA
Zhang, D., Zhang, Y., Zhao, C., Zhang, W., Shao, G., & Zhang, H. (2016). Effect of lysophosphatidic acid on the immune inflammatory response and the connexin 43 protein in myocardial infarction. Experimental and Therapeutic Medicine, 11, 1617-1624. https://doi.org/10.3892/etm.2016.3132
MLA
Zhang, D., Zhang, Y., Zhao, C., Zhang, W., Shao, G., Zhang, H."Effect of lysophosphatidic acid on the immune inflammatory response and the connexin 43 protein in myocardial infarction". Experimental and Therapeutic Medicine 11.5 (2016): 1617-1624.
Chicago
Zhang, D., Zhang, Y., Zhao, C., Zhang, W., Shao, G., Zhang, H."Effect of lysophosphatidic acid on the immune inflammatory response and the connexin 43 protein in myocardial infarction". Experimental and Therapeutic Medicine 11, no. 5 (2016): 1617-1624. https://doi.org/10.3892/etm.2016.3132
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang D, Zhang Y, Zhao C, Zhang W, Shao G and Zhang H: Effect of lysophosphatidic acid on the immune inflammatory response and the connexin 43 protein in myocardial infarction. Exp Ther Med 11: 1617-1624, 2016.
APA
Zhang, D., Zhang, Y., Zhao, C., Zhang, W., Shao, G., & Zhang, H. (2016). Effect of lysophosphatidic acid on the immune inflammatory response and the connexin 43 protein in myocardial infarction. Experimental and Therapeutic Medicine, 11, 1617-1624. https://doi.org/10.3892/etm.2016.3132
MLA
Zhang, D., Zhang, Y., Zhao, C., Zhang, W., Shao, G., Zhang, H."Effect of lysophosphatidic acid on the immune inflammatory response and the connexin 43 protein in myocardial infarction". Experimental and Therapeutic Medicine 11.5 (2016): 1617-1624.
Chicago
Zhang, D., Zhang, Y., Zhao, C., Zhang, W., Shao, G., Zhang, H."Effect of lysophosphatidic acid on the immune inflammatory response and the connexin 43 protein in myocardial infarction". Experimental and Therapeutic Medicine 11, no. 5 (2016): 1617-1624. https://doi.org/10.3892/etm.2016.3132
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