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Notch signaling is active in normal mouse middle ear epithelial cells

  • Authors:
    • Xiang Liu
    • Hai‑Bin Sheng
    • Rui Ma
    • Juan‑Mei Yang
    • Wen‑Wei Luo
    • Xiao‑Yu Yang
    • Dong‑Dong Ren
    • Fang‑Lu Chi
  • View Affiliations / Copyright

    Affiliations: Department of Otology and Skull Base Surgery, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai 200031, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1661-1667
    |
    Published online on: March 11, 2016
       https://doi.org/10.3892/etm.2016.3146
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Abstract

Mucous cell metaplasia/hyperplasia in the middle ear epithelium is associated with the occurrence of otitis media with effusion during infections. However, the mechanism by which Notch signaling regulates cell fate in the middle ear epithelium is unclear. The aim of the present study was to elucidate this mechanism by investigating the localization of Notch receptors, such as Notch1 and Notch2, and Notch ligands, such as Jagged1, in the normal mouse middle ear epithelium (NMMEE) using immunofluorescence. Furthermore, the mRNA expression levels of Notch receptors and ligands were evaluated using reverse transcription polymerase chain reaction (PCR). The effects of the γ-secretase inhibitor N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine tert-butyl ester (DAPT) on epithelial cell proliferation were determined using 5-ethynyl-2'-deoxyuridine (EdU) staining and immunofluorescence staining of the apoptosis marker caspase-3 and the epithelial proliferation marker pan-cytokeratine. In addition, the differentiation of the NMMEE cells was characterized by evaluating the mRNA expression levels of the mucous cell‑associated genes Arg2, Muc2, Spdef, Spink4 and Tff1 using quantitative PCR. Notch1, Notch2 and Jagged1 were observed to be co‑localized throughout the mouse middle ear epithelium. Furthermore, Notch1‑4, Jagged1, Jagged2, Dll1 and Dll4 mRNAs were expressed in the NMMEE cells. The inhibition of Notch by DAPT resulted in fewer EdU‑positive cells and the upregulation of the expression levels of various mucous cell‑associated genes. The results indicate that DAPT suppresses the proliferation of NMMEE cells while promoting their differentiation into mucous cells. Therefore, DAPT may provide a specific therapeutic strategy for the reversal of multiple pathological processes that are associated with epithelium thickening in the middle ear.
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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Sheng HB, Ma R, Yang JM, Luo WW, Yang XY, Ren DD and Chi FL: Notch signaling is active in normal mouse middle ear epithelial cells. Exp Ther Med 11: 1661-1667, 2016.
APA
Liu, X., Sheng, H., Ma, R., Yang, J., Luo, W., Yang, X. ... Chi, F. (2016). Notch signaling is active in normal mouse middle ear epithelial cells. Experimental and Therapeutic Medicine, 11, 1661-1667. https://doi.org/10.3892/etm.2016.3146
MLA
Liu, X., Sheng, H., Ma, R., Yang, J., Luo, W., Yang, X., Ren, D., Chi, F."Notch signaling is active in normal mouse middle ear epithelial cells". Experimental and Therapeutic Medicine 11.5 (2016): 1661-1667.
Chicago
Liu, X., Sheng, H., Ma, R., Yang, J., Luo, W., Yang, X., Ren, D., Chi, F."Notch signaling is active in normal mouse middle ear epithelial cells". Experimental and Therapeutic Medicine 11, no. 5 (2016): 1661-1667. https://doi.org/10.3892/etm.2016.3146
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Sheng HB, Ma R, Yang JM, Luo WW, Yang XY, Ren DD and Chi FL: Notch signaling is active in normal mouse middle ear epithelial cells. Exp Ther Med 11: 1661-1667, 2016.
APA
Liu, X., Sheng, H., Ma, R., Yang, J., Luo, W., Yang, X. ... Chi, F. (2016). Notch signaling is active in normal mouse middle ear epithelial cells. Experimental and Therapeutic Medicine, 11, 1661-1667. https://doi.org/10.3892/etm.2016.3146
MLA
Liu, X., Sheng, H., Ma, R., Yang, J., Luo, W., Yang, X., Ren, D., Chi, F."Notch signaling is active in normal mouse middle ear epithelial cells". Experimental and Therapeutic Medicine 11.5 (2016): 1661-1667.
Chicago
Liu, X., Sheng, H., Ma, R., Yang, J., Luo, W., Yang, X., Ren, D., Chi, F."Notch signaling is active in normal mouse middle ear epithelial cells". Experimental and Therapeutic Medicine 11, no. 5 (2016): 1661-1667. https://doi.org/10.3892/etm.2016.3146
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