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Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro

  • Authors:
    • Guang Shan
    • Xiang‑Jun Zhou
    • Yue Xia
    • Hui‑Jun Qian
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
    Copyright: © Shan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1611-1616
    |
    Published online on: March 11, 2016
       https://doi.org/10.3892/etm.2016.3152
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Abstract

Epithelial-mesenchymal transition (EMT) induces the progression of renal tubulointerstitial fibrosis. Astragalus membranaceus (AM) is a traditional Chinese herbal medicine that has been demonstrated to exert anti-inflammatory and anti‑cancer effects, in addition to protecting and supporting the immune system. The present study investigated the effects of AM on renal fibrosis. A mouse model of unilateral ureteral obstruction (UUO) was established and treated with various concentrations of AM (100, 200 or 400 mg/kg/day). Interstitial fibrosis markedly increased in the UUO mice. AM significantly reduced the obstruction‑induced upregulation of α‑smooth muscle actin (α-SMA) and downregulation of E-cadherin in the kidneys of the UUO mice (P<0.05). Furthermore, AM treatment significantly inhibited the induction of EMT and the deposition of extracellular matrix. In addition, a transforming growth factor (TGF)‑β1‑stimulated murine renal proximal tubule cell line (NRK‑52E) was treated with various concentrations of AM (10, 20, and 40 µg/ml). E‑cadherin expression levels significantly decreased and those of α‑SMA significantly increased in NRK‑52E cells stimulated with TGF‑β1 in vitro (P<0.05). Co-treatment with AM reversed these effects (P<0.05), and AM treatment reduced TGF‑β1‑induced expression and Smad2/3 phosphorylation (P<0.05). These results suggested that AM antagonizes tubular EMT by inhibiting the Smad signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Shan G, Zhou XJ, Xia Y and Qian HJ: Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro. Exp Ther Med 11: 1611-1616, 2016.
APA
Shan, G., Zhou, X., Xia, Y., & Qian, H. (2016). Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro. Experimental and Therapeutic Medicine, 11, 1611-1616. https://doi.org/10.3892/etm.2016.3152
MLA
Shan, G., Zhou, X., Xia, Y., Qian, H."Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro". Experimental and Therapeutic Medicine 11.5 (2016): 1611-1616.
Chicago
Shan, G., Zhou, X., Xia, Y., Qian, H."Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro". Experimental and Therapeutic Medicine 11, no. 5 (2016): 1611-1616. https://doi.org/10.3892/etm.2016.3152
Copy and paste a formatted citation
x
Spandidos Publications style
Shan G, Zhou XJ, Xia Y and Qian HJ: Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro. Exp Ther Med 11: 1611-1616, 2016.
APA
Shan, G., Zhou, X., Xia, Y., & Qian, H. (2016). Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro. Experimental and Therapeutic Medicine, 11, 1611-1616. https://doi.org/10.3892/etm.2016.3152
MLA
Shan, G., Zhou, X., Xia, Y., Qian, H."Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro". Experimental and Therapeutic Medicine 11.5 (2016): 1611-1616.
Chicago
Shan, G., Zhou, X., Xia, Y., Qian, H."Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro". Experimental and Therapeutic Medicine 11, no. 5 (2016): 1611-1616. https://doi.org/10.3892/etm.2016.3152
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