Open Access

Expression and function of hematopoiesis-stimulating factor receptors on the GPI‑ and GPI+ hematopoietic stem cells of patients with paroxysmal nocturnal hemoglobinuria/aplastic anemia syndrome

  • Authors:
    • Rong Fu
    • Shao‑Xue Ding
    • Yi Liu
    • Li‑Juan Li
    • Hui Liu
    • Hong‑Lei Wang
    • Tian Zhang
    • Zong‑Hong Shao
  • View Affiliations

  • Published online on: March 15, 2016     https://doi.org/10.3892/etm.2016.3163
  • Pages: 1668-1672
  • Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Paroxysmal nocturnal hemoglobinuria/aplastic anemia (PNH/AA) syndrome presents a markedly increased population of cells deficient in glycophosphatidylinositol (GPI‑ cells) and signs of bone marrow failure, which requires treatment with hematopoiesis‑stimulating factors, such as granulocyte colony‑stimulating factor (G‑CSF) and stem cell factor (SCF). However, little is known about the effects of these stimulating factors on GPI‑ cells. In order to explore the effects of stimulating factors in PNH/AA, G‑CSF receptor (CD114) and SCF receptor (CD117) expression levels on GPI+ and GPI‑ hematopoietic stem cells (HSCs) were measured by flow cytometry (FCM). The mean fluorescence intensity (MFI) values of signal transducer and activator of transcription 5 (STAT5) and phosphorylated (P)‑STAT5 were measured in GPI+ and GPI‑ HSCs by FCM following stimulation with G‑CSF or SCF in vitro. The expression levels of CD114 and CD117 on GPI‑ HSCs were significantly lower (P<0.01) than those on GPI+ HSCs in PNH/AA patients and normal controls. The MFI values of STAT5 in the GPI‑ and GPI+ HSCs of PNH/AA patients and normal controls were not significantly different. However, the MFI values of P‑STAT5 in the GPI‑ HSCs of PNH/AA patients were significantly lower than those in the GPI+ HSCs of PNH/AA patients and normal controls prior to and following stimulation with G‑CSF or SCF (P<0.01). The GPI‑ HSCs of PNH/AA patients responded poorly to stimulation by hematopoiesis-stimulating factors, which indicates that these factors can be used safely in patients with PNH/AA.
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May-2016
Volume 11 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Fu R, Ding SX, Liu Y, Li LJ, Liu H, Wang HL, Zhang T and Shao ZH: Expression and function of hematopoiesis-stimulating factor receptors on the GPI‑ and GPI+ hematopoietic stem cells of patients with paroxysmal nocturnal hemoglobinuria/aplastic anemia syndrome. Exp Ther Med 11: 1668-1672, 2016
APA
Fu, R., Ding, S., Liu, Y., Li, L., Liu, H., Wang, H. ... Shao, Z. (2016). Expression and function of hematopoiesis-stimulating factor receptors on the GPI‑ and GPI+ hematopoietic stem cells of patients with paroxysmal nocturnal hemoglobinuria/aplastic anemia syndrome. Experimental and Therapeutic Medicine, 11, 1668-1672. https://doi.org/10.3892/etm.2016.3163
MLA
Fu, R., Ding, S., Liu, Y., Li, L., Liu, H., Wang, H., Zhang, T., Shao, Z."Expression and function of hematopoiesis-stimulating factor receptors on the GPI‑ and GPI+ hematopoietic stem cells of patients with paroxysmal nocturnal hemoglobinuria/aplastic anemia syndrome". Experimental and Therapeutic Medicine 11.5 (2016): 1668-1672.
Chicago
Fu, R., Ding, S., Liu, Y., Li, L., Liu, H., Wang, H., Zhang, T., Shao, Z."Expression and function of hematopoiesis-stimulating factor receptors on the GPI‑ and GPI+ hematopoietic stem cells of patients with paroxysmal nocturnal hemoglobinuria/aplastic anemia syndrome". Experimental and Therapeutic Medicine 11, no. 5 (2016): 1668-1672. https://doi.org/10.3892/etm.2016.3163