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Article

Biological significance of PinX1 telomerase inhibitor in esophageal carcinoma treatment

  • Authors:
    • Xiang‑Kui Fan
    • Rui‑Hua Yan
    • Xiang‑Qun Geng
    • Jing‑Shan Li
    • Xiang‑Ming Chen
    • Jian‑Zhe Li
  • View Affiliations / Copyright

    Affiliations: Department of Tumor Surgery, Tai'an Central Hospital, Tai'an, Shandong 271000, P.R. China, Coal Workers' Sanatorium of Taishan, Tai'an, Shandong 271000, P.R. China
  • Pages: 2196-2200
    |
    Published online on: August 1, 2016
       https://doi.org/10.3892/etm.2016.3561
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Abstract

In the present study, to investigate the expression of PinX1 gene and its functional effects in human esophageal carcinoma (Eca)-109 cell line, expression vectors of human PinX1 (pEGFP-C3-PinX1) and its small interfering RNA (PinX1‑FAM‑siRNA) were constructed and transfected into Eca‑109 cells using Lipofectamine 2000. Firstly, the mRNA expression level of PinX1 was examined using reverse transcription‑polymerase chain reaction (RT‑PCR). Once successful transfection was achieved, the effects on the mRNA level of human telomerase reverse transcriptase (hTERT), telomerase activity, cell proliferation and apoptosis were examined by semi‑quantitative RT‑PCR, stretch PCR, MTT assay and flow cytometry, respectively. Analysis of restriction and sequencing demonstrated that the recombining plasmids were successfully constructed. The results also indicated that transfection with pEGFP‑C3‑PinX1 and PinX1‑FAM‑siRNA into Eca‑109 cells significantly increased PinX1 mRNA, decreased hTERT mRNA by 29.9% (P<0.05), and significantly reduced telomerase activity (P<0.05), inhibited cell growth, and increased the cell apoptotic index from 19.27±0.76 to 49.73±2%. The transfected PinX1‑FAM‑SiRNA exhibited PinX1 mRNA expression levels that were significantly decreased by 70% (P<0.05), whereas the remaining characteristics of Eca‑109 cells, including cell growth, mRNA level of hTERT, telomerase activity and cell apoptotic index were not altered. Exogenous PinX1 has been demonstrated to be highly expressed in human Eca. PinX1 can inhibit human telomerase activity and the expression of hTERT mRNA, reduce tumor cell growth and induce apoptosis. Notably, these inhibitory functions were inhibited by silencing PinX1 in Eca with PinX1‑FAM‑siRNA. PinX1 was successfully increased and decreased in the present study, demonstrating that it may be a potential telomerase activity inhibitor. As PinX1 is an endogenous telomerase inhibitor, it may be used as a novel tumor-targeted gene therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Fan XK, Yan RH, Geng XQ, Li JS, Chen XM and Li JZ: Biological significance of PinX1 telomerase inhibitor in esophageal carcinoma treatment. Exp Ther Med 12: 2196-2200, 2016.
APA
Fan, X., Yan, R., Geng, X., Li, J., Chen, X., & Li, J. (2016). Biological significance of PinX1 telomerase inhibitor in esophageal carcinoma treatment. Experimental and Therapeutic Medicine, 12, 2196-2200. https://doi.org/10.3892/etm.2016.3561
MLA
Fan, X., Yan, R., Geng, X., Li, J., Chen, X., Li, J."Biological significance of PinX1 telomerase inhibitor in esophageal carcinoma treatment". Experimental and Therapeutic Medicine 12.4 (2016): 2196-2200.
Chicago
Fan, X., Yan, R., Geng, X., Li, J., Chen, X., Li, J."Biological significance of PinX1 telomerase inhibitor in esophageal carcinoma treatment". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2196-2200. https://doi.org/10.3892/etm.2016.3561
Copy and paste a formatted citation
x
Spandidos Publications style
Fan XK, Yan RH, Geng XQ, Li JS, Chen XM and Li JZ: Biological significance of PinX1 telomerase inhibitor in esophageal carcinoma treatment. Exp Ther Med 12: 2196-2200, 2016.
APA
Fan, X., Yan, R., Geng, X., Li, J., Chen, X., & Li, J. (2016). Biological significance of PinX1 telomerase inhibitor in esophageal carcinoma treatment. Experimental and Therapeutic Medicine, 12, 2196-2200. https://doi.org/10.3892/etm.2016.3561
MLA
Fan, X., Yan, R., Geng, X., Li, J., Chen, X., Li, J."Biological significance of PinX1 telomerase inhibitor in esophageal carcinoma treatment". Experimental and Therapeutic Medicine 12.4 (2016): 2196-2200.
Chicago
Fan, X., Yan, R., Geng, X., Li, J., Chen, X., Li, J."Biological significance of PinX1 telomerase inhibitor in esophageal carcinoma treatment". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2196-2200. https://doi.org/10.3892/etm.2016.3561
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