Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
October-2016 Volume 12 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2016 Volume 12 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

HBD‑3 regulation of the immune response and the LPS/TLR4‑mediated signaling pathway

  • Authors:
    • Chen Zhu
    • Ni‑Rong Bao
    • Shuo Chen
    • Jian‑Ning Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2150-2154
    |
    Published online on: August 9, 2016
       https://doi.org/10.3892/etm.2016.3579
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the study was to investigate the mechanisms of human β‑defensin 3 (HBD‑3) regulation of the immune response and the lipopolysaccharide/Toll‑like receptor‑4 (LPS/TLR4)‑mediated signaling pathway. A TLR4 extracellular gene fragment was cloned into the pET32a plasmid to determine its expression in Escherichia coli (E. coli) and purification. A dialysis labeling method was used to stain HBD‑3 with fluorescein isothiocyanate (FITC). FITC‑HBD‑3 was used to induce the differentiation of human peripheral blood mononuclear cells (MNC) into immature dendritic cells (imDC) in vitro. Binding reactions were established using FITC‑HBD‑3 and sTLR4 into cell suspensions. Flow cyto­metry (FCM) was used to analyze the results. Western blot analysis confirmed the identity of nuclear factor‑κB (NF‑κB) and was used to quantify its nuclear translocation. The results showed that, HBD‑3 bound to imDC in a Ca2+‑dependent manner, and sTLR4 and LPS competitively inhibited the binding. HBD‑3 competitively blocked the binding of LPS and imDC by binding to imDC. HBD‑3 significantly decreased the translocation of LPS‑induced NF‑κB into the nucleus. In conclusion, HBD‑3 can competitively inhibit the binding of LPS and imDC through its binding to TLR4 molecules, which are expressed in imDC, thereby preventing LPS from inducing the maturity of the imDCs.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Montanaro L, Speziale P, Campoccia D, Ravaioli S, Cangini I, Pietrocola G, Giannini S and Arciola CR: Scenery of Staphylococcus implant infections in orthopedics. Future Microbiol. 6:1329–1349. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Zhu C, Tan H, Cheng T, Shen H, Shao J, Guo Y, Shi S and Zhang X: Human β-defensin 3 inhibits antibiotic-resistant Staphylococcus biofilm formation. J Surg Res. 183:204–213. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Ning R, Zhang X, Guo X and Li Q: Staphylococcus aureus regulates secretion of interleukin-6 and monocyte chemoattractant protein-1 through activation of nuclear factor kappaB signaling pathway in human osteoblasts. Braz J Infect Dis. 15:189–194. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Jarczak J, Kościuczuk EM, Lisowski P, Strzałkowska N, Jóźwik A, Horbańczuk J, Krzyżewski J, Zwierzchowski L and Bagnicka E: Defensins: natural component of human innate immunity. Hum Immunol. 74:1069–1079. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Zhu C, He N, Cheng T, Tan H, Guo Y, Chen D, Cheng M, Yang Z and Zhang X: Ultrasound-targeted microbubble destruction enhances human β-defensin 3 activity against antibiotic-resistant Staphylococcus biofilms. Inflammation. 36:983–996. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Hinrichsen K, Podschun R, Schubert S, Schröder JM, Harder J and Proksch E: Mouse beta-defensin-14, an antimicrobial ortholog of human beta-defensin-3. Antimicrob Agents Chemother. 52:1876–1879. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Röhrl J, Yang D, Oppenheim JJ and Hehlgans T: Identification and biological characterization of mouse beta-defensin 14, the orthologue of human beta-defensin 3. J Biol Chem. 283:5414–5419. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Hancock RE and Sahl HG: Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. Nat Biotechnol. 24:1551–1557. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Shi Y, Song W, Feng ZH, Zhao YT, Li F, Tian Y and Zhao YM: Disinfection of maxillofacial silicone elastomer using a novel antimicrobial agent: recombinant human beta-defensin-3. Eur J Clin Microbiol Infect Dis. 28:415–420. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Varoga D, Wruck CJ, Tohidnezhad M, Brandenburg L, Paulsen F, Mentlein R, Seekamp A, Besch L and Pufe T: Osteoblasts participate in the innate immunity of the bone by producing human beta defensin-3. Histochem Cell Biol. 131:207–218. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Semple F, MacPherson H, Webb S, Cox SL, Mallin LJ, Tyrrell C, Grimes GR, Semple CA, Nix MA, Millhauser GL, et al: Human β-defensin 3 affects the activity of pro-inflammatory pathways associated with MyD88 and TRIF. Eur J Immunol. 41:3291–3300. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Röhrl J, Yang D, Oppenheim JJ and Hehlgans T: Human beta-defensin 2 and 3 and their mouse orthologs induce chemotaxis through interaction with CCR2. J Immunol. 184:6688–6694. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Zhu C, Qin H, Cheng T, Tan HL, Guo YY, Shi SF, Chen DS and Zhang XL: Staphylococcus aureus supernatant induces the release of mouse β-defensin-14 from osteoblasts via the p38 MAPK and NF-κB pathways. Int J Mol Med. 31:1484–1494. 2013.PubMed/NCBI

14 

Zhu C, Wang J, Cheng T, Li Q, Shen H, Qin H, Cheng M and Zhang X: The potential role of increasing the release of mouse β-defensin-14 in the treatment of osteomyelitis in mice: a primary study. PLoS One. 9:e868742014. View Article : Google Scholar : PubMed/NCBI

15 

Wang M, Chen Y, Zhang Y, Zhang L, Lu X and Chen Z: Mannan-binding lectin directly interacts with Toll-like receptor 4 and suppresses lipopolysaccharide-induced inflammatory cytokine secretion from THP-1 cells. Cell Mol Immunol. 8:265–275. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Spadaro M, Montone M, Arigoni M, Cantarella D, Forni G, Pericle F, Pascolo S, Calogero RA and Cavallo F: Recombinant human lactoferrin induces human and mouse dendritic cell maturation via Toll-like receptors 2 and 4. FASEB J. 28:416–429. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhu C, Bao NR, Chen S and Zhao JN: HBD‑3 regulation of the immune response and the LPS/TLR4‑mediated signaling pathway. Exp Ther Med 12: 2150-2154, 2016.
APA
Zhu, C., Bao, N., Chen, S., & Zhao, J. (2016). HBD‑3 regulation of the immune response and the LPS/TLR4‑mediated signaling pathway. Experimental and Therapeutic Medicine, 12, 2150-2154. https://doi.org/10.3892/etm.2016.3579
MLA
Zhu, C., Bao, N., Chen, S., Zhao, J."HBD‑3 regulation of the immune response and the LPS/TLR4‑mediated signaling pathway". Experimental and Therapeutic Medicine 12.4 (2016): 2150-2154.
Chicago
Zhu, C., Bao, N., Chen, S., Zhao, J."HBD‑3 regulation of the immune response and the LPS/TLR4‑mediated signaling pathway". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2150-2154. https://doi.org/10.3892/etm.2016.3579
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu C, Bao NR, Chen S and Zhao JN: HBD‑3 regulation of the immune response and the LPS/TLR4‑mediated signaling pathway. Exp Ther Med 12: 2150-2154, 2016.
APA
Zhu, C., Bao, N., Chen, S., & Zhao, J. (2016). HBD‑3 regulation of the immune response and the LPS/TLR4‑mediated signaling pathway. Experimental and Therapeutic Medicine, 12, 2150-2154. https://doi.org/10.3892/etm.2016.3579
MLA
Zhu, C., Bao, N., Chen, S., Zhao, J."HBD‑3 regulation of the immune response and the LPS/TLR4‑mediated signaling pathway". Experimental and Therapeutic Medicine 12.4 (2016): 2150-2154.
Chicago
Zhu, C., Bao, N., Chen, S., Zhao, J."HBD‑3 regulation of the immune response and the LPS/TLR4‑mediated signaling pathway". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2150-2154. https://doi.org/10.3892/etm.2016.3579
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team