MicroRNA-129-5p inhibits vascular smooth muscle cell proliferation by targeting Wnt5a

Zhang,Yiming; Liu,Zhao; Zhou,Min; Liu,Changjian

doi:10.3892/etm.2016.3672

MicroRNA-129-5p inhibits vascular smooth muscle cell proliferation by targeting Wnt5a

Authors:
- Yiming Zhang
- Zhao Liu
- Min Zhou
- Changjian Liu
View Affiliations

Affiliations: Department of Vascular Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China
Published online on: September 6, 2016 https://doi.org/10.3892/etm.2016.3672
Pages: 2651-2656

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Aberrant smooth muscle cells (SMCs) play important roles in the formation of abdominal aortic aneurysm (AAA). Although the molecular mechanism of AAA formation has been investigated, there is a lack of understanding concerning the role of microRNAs (miRNAs) in AAA, which the current study aimed to address. Firstly, miRNA array analysis was performed in order to compare the miRNA profiles in a mouse model of AAA with those in normal control mice, and differentially expressed miRNAs were identified. miR‑129‑5p was selected for further analysis, and was used to transfect human SMCs. The results of an MTT assay revealed that miR‑129‑5p inhibited the proliferation of SMCs, and flow cytometry indicated that apoptosis was induced. Bioinformatic analysis predicted that Wnt5a was the potential target gene of miR‑129‑5p, and this was verified by luciferase assay. In summary, miR‑129‑5p inhibits cellular proliferation, induces apoptosis and modulates the Wnt5a signaling pathway in SMCs.

View Figures

View References

1	Kim CW, Kumar S, Son DJ, Jang IH, Griendling KK and Jo H: Prevention of abdominal aortic aneurysm by anti-microRNA-712 or anti-microRNA-205 in angiotensin II-infused mice. Arterioscler Thromb Vasc Biol. 34:1412–1421. 2014. View Article : Google Scholar : PubMed/NCBI
2	Biros E, Moran CS, Wang Y, Walker PJ, Cardinal J and Golledge J: MicroRNA profiling in patients with abdominal aortic aneurysms: The significance of miR-155. Clin Sci (Lond). 126:795–803. 2014. View Article : Google Scholar : PubMed/NCBI
3	Maegdefessel L, Dalman RL and Tsao PS: Pathogenesis of abdominal aortic aneurysms: MicroRNAs, proteases, genetic associations. Annu Rev Med. 65:49–62. 2014. View Article : Google Scholar : PubMed/NCBI
4	Maegdefessel L, Azuma J and Tsao PS: MicroRNA-29b regulation of abdominal aortic aneurysm development. Trends Cardiovasc Med. 24:1–6. 2014. View Article : Google Scholar : PubMed/NCBI
5	Maegdefessel L, Spin JM, Adam M, Raaz U, Toh R, Nakagami F and Tsao PS: Micromanaging abdominal aortic aneurysms. Int J Mol Sci. 14:14374–14394. 2013. View Article : Google Scholar : PubMed/NCBI
6	Cheuk BLY and Cheng SWK: Identification and characterization of microRNAs in vascular smooth muscle cells from patients with abdominal aortic aneurysms. J Vasc Surg. 59:20–209. 2014. View Article : Google Scholar
7	Adam M, Raaz U, Spin JM and Tsao PS: MicroRNAs in abdominal aortic aneurysm. Curr Vasc Pharmacol. 13:280–290. 2015. View Article : Google Scholar : PubMed/NCBI
8	Lee HJ, Yi JS, Lee HJ, Lee IW, Park KC and Yang JH: Dysregulated expression profiles of MicroRNAs of experimentally induced cerebral aneurysms in rats. J sKorean Neurosurg Soc. 53:72–76. 2013. View Article : Google Scholar
9	Golledge J and Kuivaniemi H: Genetics of abdominal aortic aneurysm. Curr Opin Cardiol. 28:290–296. 2013. View Article : Google Scholar : PubMed/NCBI
10	Kin K, Miyagawa S, Fukushima S, Shirakawa Y, Torikai K, Shimamura K, Daimon T, Kawahara Y, Kuratani T and Sawa Y: Tissue- and plasma-specific MicroRNA signatures for atherosclerotic abdominal aortic aneurysm. J Am Heart Assoc. 1:e0007452012. View Article : Google Scholar : PubMed/NCBI
11	Pahl MC, Derr K, Gäbel G, Hinterseher I, Elmore JR, Schworer CM, Peeler TC, Franklin DP, Gray JL, Carey DJ, et al: MicroRNA expression signature in human abdominal aortic aneurysms. BMC Med Genomics. 5:252012. View Article : Google Scholar : PubMed/NCBI
12	Maegdefessel L, Azuma J, Toh R, Deng A, Merk DR, Raiesdana A, Leeper NJ, Raaz U, Schoelmerich AM, McConnell MV, et al: MicroRNA-21 blocks abdominal aortic aneurysm development and nicotine-augmented expansion. Sci Transl Med. 4:122ra222012. View Article : Google Scholar : PubMed/NCBI
13	Maegdefessel L, Azuma J, Toh R, Merk DR, Deng A, Chin JT, Raaz U, Schoelmerich AM, Raiesdana A, Leeper NJ, et al: Inhibition of microRNA-29b reduces murine abdominal aortic aneurysm development. J Clin Invest. 122:497–506. 2012. View Article : Google Scholar : PubMed/NCBI
14	Milewicz DM: MicroRNAs, fibrotic remodeling and aortic aneurysms. J Clin Invest. 122:490–493. 2012. View Article : Google Scholar : PubMed/NCBI
15	Liu G, Huang Y, Lu X, Lu M, Huang X, Li W and Jiang M: Identification and characteristics of microRNAs with altered expression patterns in a rat model of abdominal aortic aneurysms. Tohoku J Exp Med. 222:187–193. 2010. View Article : Google Scholar : PubMed/NCBI
16	Mill C, Monk BA, Williams H, Simmonds SJ, Jeremy JY, Johnson JL and George SJ: Wnt5a-induced Wnt1-inducible secreted protein-1 suppresses vascular smooth muscle cell apoptosis induced by oxidative stress. Arterioscler Thromb Vasc Biol. 34:2449–2456. 2014. View Article : Google Scholar : PubMed/NCBI
17	Jin Y, Wang W, Chai S, Liu J, Yang T and Wang J: Wnt5a attenuates hypoxia-induced pulmonary arteriolar remodeling and right ventricular hypertrophy in mice. Exp Biol Med (Maywood). 240:1742–1751. 2015. View Article : Google Scholar : PubMed/NCBI
18	Guan S, Wang Z, Xin F and Xin H: Wnt5a is associated with the differentiation of bone marrow mesenchymal stem cells in vascular calcification by connecting with different receptors. Mol Med Rep. 10:1985–1991. 2014.PubMed/NCBI
19	Zhu H, He J, Ye L, Lin F, Hou J, Zhong Y and Jiang W: Mechanisms of angiogenesis in a Curculigoside A-treated rat model of cerebral ischemia and reperfusion injury. Toxicol Appl Pharmacol. 288:313–321. 2015. View Article : Google Scholar : PubMed/NCBI
20	Guan S, Wang Z, Xin F and Xin H: Wnt5a is associated with the differentiation of bone marrow mesenchymal stem cells in vascular calcification by connecting with different receptors. Mol Med Rep. 10:1985–1991. 2014.PubMed/NCBI