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Article

MicroRNA-129-5p inhibits vascular smooth muscle cell proliferation by targeting Wnt5a

  • Authors:
    • Yiming Zhang
    • Zhao Liu
    • Min Zhou
    • Changjian Liu
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China
  • Pages: 2651-2656
    |
    Published online on: September 6, 2016
       https://doi.org/10.3892/etm.2016.3672
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Abstract

Aberrant smooth muscle cells (SMCs) play important roles in the formation of abdominal aortic aneurysm (AAA). Although the molecular mechanism of AAA formation has been investigated, there is a lack of understanding concerning the role of microRNAs (miRNAs) in AAA, which the current study aimed to address. Firstly, miRNA array analysis was performed in order to compare the miRNA profiles in a mouse model of AAA with those in normal control mice, and differentially expressed miRNAs were identified. miR‑129‑5p was selected for further analysis, and was used to transfect human SMCs. The results of an MTT assay revealed that miR‑129‑5p inhibited the proliferation of SMCs, and flow cytometry indicated that apoptosis was induced. Bioinformatic analysis predicted that Wnt5a was the potential target gene of miR‑129‑5p, and this was verified by luciferase assay. In summary, miR‑129‑5p inhibits cellular proliferation, induces apoptosis and modulates the Wnt5a signaling pathway in SMCs.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Liu Z, Zhou M and Liu C: MicroRNA-129-5p inhibits vascular smooth muscle cell proliferation by targeting Wnt5a. Exp Ther Med 12: 2651-2656, 2016.
APA
Zhang, Y., Liu, Z., Zhou, M., & Liu, C. (2016). MicroRNA-129-5p inhibits vascular smooth muscle cell proliferation by targeting Wnt5a. Experimental and Therapeutic Medicine, 12, 2651-2656. https://doi.org/10.3892/etm.2016.3672
MLA
Zhang, Y., Liu, Z., Zhou, M., Liu, C."MicroRNA-129-5p inhibits vascular smooth muscle cell proliferation by targeting Wnt5a". Experimental and Therapeutic Medicine 12.4 (2016): 2651-2656.
Chicago
Zhang, Y., Liu, Z., Zhou, M., Liu, C."MicroRNA-129-5p inhibits vascular smooth muscle cell proliferation by targeting Wnt5a". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2651-2656. https://doi.org/10.3892/etm.2016.3672
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Liu Z, Zhou M and Liu C: MicroRNA-129-5p inhibits vascular smooth muscle cell proliferation by targeting Wnt5a. Exp Ther Med 12: 2651-2656, 2016.
APA
Zhang, Y., Liu, Z., Zhou, M., & Liu, C. (2016). MicroRNA-129-5p inhibits vascular smooth muscle cell proliferation by targeting Wnt5a. Experimental and Therapeutic Medicine, 12, 2651-2656. https://doi.org/10.3892/etm.2016.3672
MLA
Zhang, Y., Liu, Z., Zhou, M., Liu, C."MicroRNA-129-5p inhibits vascular smooth muscle cell proliferation by targeting Wnt5a". Experimental and Therapeutic Medicine 12.4 (2016): 2651-2656.
Chicago
Zhang, Y., Liu, Z., Zhou, M., Liu, C."MicroRNA-129-5p inhibits vascular smooth muscle cell proliferation by targeting Wnt5a". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2651-2656. https://doi.org/10.3892/etm.2016.3672
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