Preliminary results of a non-invasive method to measure tumor size and distribution in vivo

Fu,Shu‑Ling; Chen,Chien‑An; Hung,Ling‑Chien; Lee,Moon‑Sing; Chiou,Wen‑Yen; Lin,Hon‑Yi; Su,Yu‑Chieh; Lee,Ching‑Chih; Hung,Shih‑Kai

doi:10.3892/etm.2016.3819

Preliminary results of a non-invasive method to measure tumor size and distribution in vivo

Authors:
- Shu‑Ling Fu
- Chien‑An Chen
- Ling‑Chien Hung
- Moon‑Sing Lee
- Wen‑Yen Chiou
- Hon‑Yi Lin
- Yu‑Chieh Su
- Ching‑Chih Lee
- Shih‑Kai Hung
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Affiliations: Institute of Traditional Medicine, National Yang‑Ming University, Taipei 11221, Taiwan, R.O.C., Department of Radiation Oncology, Zhongxing Branch, Taipei City Hospital, Taipei 10341, Taiwan, R.O.C., Department of Radiation Oncology, Buddhist Dalin Tzu Chi Hospital, Chiayi 62247, Taiwan, R.O.C., School of Medicine, Tzu Chi University, Hualian 97061, Taiwan, R.O.C.
Published online on: October 19, 2016 https://doi.org/10.3892/etm.2016.3819
Pages: 3614-3620
Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Molecular imaging of reporter gene expression in cancer cells can provide rapid, sensitive and non-invasive monitoring of tumor behavior. The aim of the present study was to establish a non‑invasive method to measure tumor size and distribution in vivo. Briefly, H-Ras-transformed cells were stably transfected with a plasmid containing the luciferase gene (Luc), designated as Ras/Luc. Ras/Luc cells were injected into the back or tail vein of nude BALB/cAnN-Foxn1nu/CrlNarl mice (age, 6‑8 weeks). Mice were subsequently administered D‑luciferin via intra‑peritoneal injection, prior to image acquisition. Photons emitted from the mice were detected via an imaging system. Tumor size and distribution in vivo were quantified as photons/second. Andrographolide has demonstrated radiosensitization in previous in vitro and in vivo studies. In the present study, the potential effects of andrographolide cancer metastasis were investigated further, using an imaging system. Preliminary results of andrographolide combined with radiation indicated the inhibition of cancer metastasis. The present mechanistic study of andrographolide‑mediated effects demonstrated that activated extracellular signal regulated kinase protein and H2O2 production levels were significantly increased by andrographolide. In summary, the present study established a non‑invasive method to measure tumor size and distribution in vivo and indicated that andrographolide may be a potential therapeutic strategy in cancer therapy.

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