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Article

MicroRNA-212 targets FOXA1 and suppresses the proliferation and invasion of intrahepatic cholangiocarcinoma cells

Retraction in: /10.3892/etm.2017.4226
  • Authors:
    • Lei Zhu
    • Feizhou Huang
    • Gang Deng
    • Wanpin Nie
    • Wei Huang
    • Hongbo Xu
    • Shaopeng Zheng
    • Zhongjie Yi
    • Tao Wan
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary and Pancreatic Surgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China
  • Pages: 3790-3796
    |
    Published online on: October 19, 2016
       https://doi.org/10.3892/etm.2016.3824
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Abstract

MicroRNAs (miRNAs), which are a class of small RNAs, have been shown to negatively regulate the expression of their target genes by directly binding to the 3'‑untranslated region (3'‑UTR) of mRNA. miRNA dysregulation has been associated with the pathogenesis of numerous types of human cancer. However, the role of miRNAs in intrahepatic cholangiocarcinoma (ICC) has yet to be fully elucidated. The present study aimed to investigate the role of miR‑212 in the growth and metastasis of ICC in vitro, as well as the underlying mechanism. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blotting were used to examine mRNA and protein expression. An MTT assay and transwell assay were conducted to determine cell proliferation and invasion rates. The results of the RT‑qPCR demonstrated that miR‑212 was downregulated in the majority of investigated ICC tissues, as compared with their matched adjacent non‑tumor tissues. In addition, miR‑212 expression was shown to be markedly downregulated in three ICC cell lines, as compared with human intrahepatic biliary epithelial cells. Furthermore, restoration of miR‑212 expression significantly suppressed the proliferation and invasion of ICC QBC939 cells. Forkhead box protein A1 (FOXA1) was predicted to be a putative target of miR‑212 by bioinformatics analysis with TargetScan. Therefore, a luciferase reporter assay was conducted to confirm that miR‑212 was able to directly bind to the 3'‑UTR of FOXA1 mRNA. In addition, using western blot analysis, the protein expression of FOXA1 was shown to be negatively regulated by miR‑212 in ICC QBC939 cells. In conclusion, it was demonstrated that FOXA1 was frequently upregulated in various ICC tissues and cell lines. The results of the present study suggested that miR‑212 inhibits the proliferation and invasion of ICC cells by directly targeting FOXA1, and thus may be considered a potential candidate for the treatment of ICC.
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1 

Andersen JB: Molecular pathogenesis of intrahepatic cholangiocarcinoma. J Hepatobiliary Pancreat Sci. 22:101–113. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Sempoux C, Jibara G, Ward SC, Fan C, Qin L, Roayaie S, Fiel MI, Schwartz M and Thung SN: Intrahepatic cholangiocarcinoma: New insights in pathology. Semin Liver Dis. 31:49–60. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Sanada Y, Kawashita Y, Okada S, Azuma T and Matsuo S: Review to better understand the macroscopic subtypes and histogenesis of intrahepatic cholangiocarcinoma. World J Gastrointest Pathophysiol. 5:188–199. 2014.PubMed/NCBI

4 

Long XR, He Y, Huang C and Li J: MicroRNA-148a is silenced by hypermethylation and interacts with DNA methyltransferase 1 in hepatocellular carcinogenesis. Int J Oncol. 44:1915–1922. 2014.PubMed/NCBI

5 

Tamagawa S, Beder LB, Hotomi M, Gunduz M, Yata K, Grenman R and Yamanaka N: Role of miR-200c/miR-141 in the regulation of epithelial-mesenchymal transition and migration in head and neck squamous cell carcinoma. Int J Mol Med. 33:879–886. 2014.PubMed/NCBI

6 

Wan X, Cheng Q, Peng R, Ma Z, Chen Z, Cao Y and Jiang B: ROCK1, a novel target of miR-145, promotes glioma cell invasion. Mol Med Rep. 9:1877–1882. 2014.PubMed/NCBI

7 

Liu X, Yan S, Pei C and Cui Y: Decreased microRNA-132 and its function in human non-small cell lung cancer. Mol Med Rep. 11:3601–3608. 2015.PubMed/NCBI

8 

Zheng K, Liu W, Liu Y, Jiang C and Qian Q: MicroRNA-133a suppresses colorectal cancer cell invasion by targeting Fascin1. Oncol Lett. 9:869–874. 2015.PubMed/NCBI

9 

Chen P, Zeng M, Zhao Y and Fang X: Upregulation of Limk1 caused by microRNA-138 loss aggravates the metastasis of ovarian cancer by activation of Limk1/cofilin signaling. Oncol Rep. 32:2070–2076. 2014.PubMed/NCBI

10 

Tavolaro S, Colombo T, Chiaretti S, Peragine N, Fulci V, Ricciardi MR, Messina M, Bonina S, Brugnoletti F, Marinelli M, et al: Increased chronic lymphocytic leukemia proliferation upon IgM stimulation is sustained by the upregulation of miR-132 and miR-212. Genes Chromosomes Cancer. 54:222–234. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Qi B, Liu SG, Qin XG, Yao WJ, Lu JG, Guo L, Wang TY, Li HC and Zhao BS: Overregulation of microRNA-212 in the poor prognosis of esophageal cancer patients. Genet Mol Res. 13:7800–7807. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Jiang X, Chen X, Chen L, Ma Y, Zhou L, Qi Q, Liu Y, Zhang S, Luo J and Zhou X: Upregulation of the miR-212/132 cluster suppresses proliferation of human lung cancer cells. Oncol Rep. 33:705–712. 2015.PubMed/NCBI

13 

Liang X, Zeng J, Wang L, Fang M, Wang Q, Zhao M, Xu X, Liu Z, Li W, Liu S, et al: Histone demethylase retinoblastoma binding protein 2 is overexpressed in hepatocellular carcinoma and negatively regulated by hsa-miR-212. PLoS One. 8:e697842013. View Article : Google Scholar : PubMed/NCBI

14 

Ma C, Nong K, Wu B, Dong B, Bai Y, Zhu H, Wang W, Huang X, Yuan Z and Ai K: miR-212 promotes pancreatic cancer cell growth and invasion by targeting the hedgehog signaling pathway receptor patched-1. J Exp Clin Cancer Res. 33:542014. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Fabbri M, Calore F, Paone A, Galli R and Calin GA: Epigenetic regulation of miRNAs in cancer. Adv Exp Med Biol. 754:137–148. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Bouyssou JM, Manier S, Huynh D, Issa S, Roccaro AM and Ghobrial IM: Regulation of microRNAs in cancer metastasis. Biochim Biophys Acta. 1845:255–265. 2014.PubMed/NCBI

18 

Wen KC, Sung PL, Yen MS, Chuang CM, Liou WS and Wang PH: MicroRNAs regulate several functions of normal tissues and malignancies. Taiwan J Obstet Gynecol. 52:465–469. 2013. View Article : Google Scholar : PubMed/NCBI

19 

John K, Wu J, Lee BW and Farah CS: MicroRNAs in head and neck cancer. Int J Dent. 2013:6502182013. View Article : Google Scholar : PubMed/NCBI

20 

Yamasaki S: Intrahepatic cholangiocarcinoma: Macroscopic type and stage classification. J Hepatobiliary Pancreat Surg. 10:288–291. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Zhao XQ, Ma HX, Su MS and He L: Osteopontin promoter polymorphisms at locus −443 are associated with metastasis and poor prognosis of human intrahepatic cholangiocarcinoma in Chinese population. Int J Clin Exp Pathol. 7:6914–6921. 2014.PubMed/NCBI

22 

Park JK, Henry JC, Jiang J, Esau C, Gusev Y, Lerner MR, Postier RG, Brackett DJ and Schmittgen TD: miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor. Biochem Biophys Res Commun. 406:518–523. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Schultz NA, Andersen KK, Roslind A, Willenbrock H, Wøjdemann M and Johansen JS: Prognostic microRNAs in cancer tissue from patients operated for pancreatic cancer-five microRNAs in a prognostic index. World J Surg. 36:2699–2707. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Scapoli L, Palmieri A, Lo Muzio L, Pezzetti F, Rubini C, Girardi A, Farinella F, Mazzotta M and Carinci F: MicroRNA expression profiling of oral carcinoma identifies new markers of tumor progression. Int J Immunopathol Pharmacol. 23:1229–1234. 2010.PubMed/NCBI

25 

Wada R, Akiyama Y, Hashimoto Y, Fukamachi H and Yuasa Y: miR-212 is downregulated and suppresses methyl-CpG-binding protein MeCP2 in human gastric cancer. Int J Cancer. 127:1106–1114. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Incoronato M, Urso L, Portela A, Laukkanen MO, Soini Y, Quintavalle C, Keller S, Esteller M and Condorelli G: Epigenetic regulation of miR-212 expression in lung cancer. PLoS One. 6:e277222011. View Article : Google Scholar : PubMed/NCBI

27 

Qian X, Samadani U, Porcella A and Costa RH: Decreased expression of hepatocyte nuclear factor 3 alpha during the acute-phase response influences transthyretin gene transcription. Mol Cell Biol. 15:1364–1376. 1995. View Article : Google Scholar : PubMed/NCBI

28 

Hsiang CH, Marten NW and Straus DS: Upstream region of rat serum albumin gene promoter contributes to promoter activity: Presence of functional binding site for hepatocyte nuclear factor-3. Biochem J. 338:241–249. 1999. View Article : Google Scholar : PubMed/NCBI

29 

Guzmán C, Benet M, Pisonero-Vaquero S, Moya M, García-Mediavilla MV, Martínez-Chantar ML, González-Gallego J, Castell JV, Sánchez-Campos S and Jover R: The human liver fatty acid binding protein (FABP1) gene is activated by FOXA1 and PPARalpha; and repressed by C/EBPalpha: Implications in FABP1 down-regulation in nonalcoholic fatty liver disease. Biochim Biophys Acta. 1831:803–818. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Horimoto Y, Arakawa A, Harada-Shoji N, Sonoue H, Yoshida Y, Himuro T, Igari F, Tokuda E, Mamat O, Tanabe M, et al: Low FOXA1 expression predicts good response to neo-adjuvant chemotherapy resulting in good outcomes for luminal HER2-negative breast cancer cases. Br J Cancer. 112:345–351. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Zhang Y and Tong T: FOXA1 antagonizes EZH2-mediated CDKN2A repression in carcinogenesis. Biochem Biophys Res Commun. 453:172–178. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Yu W, Qiao Y, Tang X, Ma L, Wang Y, Zhang X, Weng W, Pan Q, Yu Y, Sun F and Wang J: Tumor suppressor long non-coding RNA, MT1DP is negatively regulated by YAP and Runx2 to inhibit FoxA1 in liver cancer cells. Cell Signal. 26:2961–2968. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Zhao Y and Li Z: Interplay of estrogen receptors and FOXA factors in the liver cancer. Mol Cell Endocrinol. S0303–S7207. 2015.

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Copy and paste a formatted citation
Spandidos Publications style
Zhu L, Huang F, Deng G, Nie W, Huang W, Xu H, Zheng S, Yi Z and Wan T: MicroRNA-212 targets FOXA1 and suppresses the proliferation and invasion of intrahepatic cholangiocarcinoma cells Retraction in /10.3892/etm.2017.4226. Exp Ther Med 12: 3790-3796, 2016.
APA
Zhu, L., Huang, F., Deng, G., Nie, W., Huang, W., Xu, H. ... Wan, T. (2016). MicroRNA-212 targets FOXA1 and suppresses the proliferation and invasion of intrahepatic cholangiocarcinoma cells Retraction in /10.3892/etm.2017.4226. Experimental and Therapeutic Medicine, 12, 3790-3796. https://doi.org/10.3892/etm.2016.3824
MLA
Zhu, L., Huang, F., Deng, G., Nie, W., Huang, W., Xu, H., Zheng, S., Yi, Z., Wan, T."MicroRNA-212 targets FOXA1 and suppresses the proliferation and invasion of intrahepatic cholangiocarcinoma cells Retraction in /10.3892/etm.2017.4226". Experimental and Therapeutic Medicine 12.6 (2016): 3790-3796.
Chicago
Zhu, L., Huang, F., Deng, G., Nie, W., Huang, W., Xu, H., Zheng, S., Yi, Z., Wan, T."MicroRNA-212 targets FOXA1 and suppresses the proliferation and invasion of intrahepatic cholangiocarcinoma cells Retraction in /10.3892/etm.2017.4226". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3790-3796. https://doi.org/10.3892/etm.2016.3824
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu L, Huang F, Deng G, Nie W, Huang W, Xu H, Zheng S, Yi Z and Wan T: MicroRNA-212 targets FOXA1 and suppresses the proliferation and invasion of intrahepatic cholangiocarcinoma cells Retraction in /10.3892/etm.2017.4226. Exp Ther Med 12: 3790-3796, 2016.
APA
Zhu, L., Huang, F., Deng, G., Nie, W., Huang, W., Xu, H. ... Wan, T. (2016). MicroRNA-212 targets FOXA1 and suppresses the proliferation and invasion of intrahepatic cholangiocarcinoma cells Retraction in /10.3892/etm.2017.4226. Experimental and Therapeutic Medicine, 12, 3790-3796. https://doi.org/10.3892/etm.2016.3824
MLA
Zhu, L., Huang, F., Deng, G., Nie, W., Huang, W., Xu, H., Zheng, S., Yi, Z., Wan, T."MicroRNA-212 targets FOXA1 and suppresses the proliferation and invasion of intrahepatic cholangiocarcinoma cells Retraction in /10.3892/etm.2017.4226". Experimental and Therapeutic Medicine 12.6 (2016): 3790-3796.
Chicago
Zhu, L., Huang, F., Deng, G., Nie, W., Huang, W., Xu, H., Zheng, S., Yi, Z., Wan, T."MicroRNA-212 targets FOXA1 and suppresses the proliferation and invasion of intrahepatic cholangiocarcinoma cells Retraction in /10.3892/etm.2017.4226". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3790-3796. https://doi.org/10.3892/etm.2016.3824
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